5 documents found, page 1 of 1
Adaptive evolution of PB1 from influenza A(H1N1)pdm09 virus towards an enhanced...
Santos, Luís A.; Almeida, Filipe; Gíria, Marta; Trigueiro-Louro, João; Rebelo-de-Andrade, HelenaPB1 influenza virus retain traces of interspecies transmission and adaptation. Previous phylogenetic analyses highlighted mutations L298I, R386K and I517V in PB1 to have putatively ameliorated the A(H1N1)pdm09 adaptation to the human host. This study aimed to evaluate the reversal of these mutations and infer the role of these residues in the virus overall fitness and adaptation. We generate PB1-mutated viruses...
Unlocking COVID therapeutic targets: A structure-based rationale against SARS-C...
Trigueiro-Louro, João; Correia, Vanessa; Figueiredo-Nunes, Inês; Gíria, Marta; Rebelo-de-Andrade, HelenaThere are no approved target therapeutics against SARS-CoV-2 or other beta-CoVs. The beta-CoV Spike protein is a promising target considering the critical role in viral infection and pathogenesis and its surface exposed features. We performed a structure-based strategy targeting highly conserved druggable regions resulting from a comprehensive large-scale sequence analysis and structural characterization of Spi...
Reverse genetics vaccine seeds for influenza: Proof of concept in the source of...
Gíria, Marta; Santos, Luís; Louro, João; Rebelo de Andrade, HelenaGrowth deficits of reverse genetics vaccine seeds have compromised effective immunization. The impairment has been attributed to sub-optimal protein interactions. Some level of dependence may exist between PB1 and antigenic glycoproteins, however, further research is necessary to clarify the extent to which it can be used in favor of seed production. Our objective was to establish proof of concept on the phenot...
Functional compatibility of PB1 and antigenic proteins as a determinant of vira...
Gíria, Marta; Louro, João; Rebelo de Andrade, HelenaObjectives: To determine if the functional compatibility between PB1 and antigenic proteins is a molecular determinant of virus growth and antigen yield in PR8:A(H1N1)pdm09 reassortants; To evaluate the phenotypic expression of reverting mutations R386K, I517V and L298I, previously identified as putatively associated with an enhanced functional compatibility between PB1 and HA in the A(H1N1)pdm09
Outbreak of acute respiratory infection among infants in Lisbon, Portugal, caus...
Andrade, Helena Rebelo de; Pereira, Cristiana; Gíria, Marta; Prudêncio, Ema; Brito, Maria João; Calé, Etelvina; Taveira, NunoHuman adenoviruses (AdVs) typically cause mild illnesses in otherwise healthy hosts. We investigated a pediatric outbreak of acute respiratory infection with fatal outcomes that occurred in Lisbon, Portugal, in 2004. Biological specimens were collected from 83 children attending two nurseries, a kinesiotherapy clinic, and the household of a nanny. Adenovirus infection was confirmed in 48 children by PCR and vir...