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Study of associations between COVID-19 and bacterial infections: a retrospectiv...

Diaz, Raquel; Afreixo, Vera; Rocha, Filipa; Nogueira, Gabriela; Ramalheira, Elmano; Gago, Bruno

The COVID-19 pandemic disease, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (Sars-CoV-2), has affected millions of people worldwide. Clinical practice highlights that the number of patients with COVID-19 diagnosed with bacterial infections during periods of hospitalization is increasing. Rapid characterization of co-infection is essential in the treatment of most patients with COVID-19, as it c...




P43 - Meta-analysis to evaluate the vancomycin MIC Creep in Staphylococcus aure...

Diaz, Raquel; Afreixo, Vera; Rodrigues, Carmen; Ramalheira, Elmano; Gago, Bruno


Rapid screening of methicillin-resistant Staphylococcus aureus carrying the mec...

Diaz, Raquel; Afreixo, Vera; Ramalheira, Elmano; Gago, Bruno

Background: The discovery of mecC gene in MRSA isolates and its report in neighboring Spain and France, advices for monitoring its presence in Portuguese hospitals since changes in local epidemiology can occur quickly. The implementation of rapid procedures based on the automated system VITEK2 allow for simple screening of mecC MRSA isolates with the profile of susceptibility to oxacillin and resistance to cefo...


Characterization of the medical department of pharmaceutical companies operatin...

Gala, Acílio; Lima, Ana Rita; Laranjeira, Luís; Ramos, Miguel; Guimarães, João Paulo; Sousa, Conceição; Leitão, Andreia; de Jesus, Paula Martins

In a context of strong regulatory demands and high economic pressures and constraints, medical departments within pharmaceutical companies, have demonstrated its strategic relevance even though the functions to embrace are not globally consensual.In this context, a study that intended to analyse the management of medical activities, in pharmaceutical companies operating in Portugal, including allocated human re...


The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and eth...

Gago, Bruno; Nyström, Thomas; Cavaleiro, Carlos; Rocha, Bárbara S.; Barbosa, Rui M.; Laranjinha, João; Lundberg, Jon O.

By acting as a bioreactor, affording chemical and mechanical conditions for the reaction between dietary components, the stomach may be a source of new bioactive molecules. Using gas chromatography-mass spectrometry we here demonstrate that, under acidic gastric conditions, ethyl nitrite is formed in µM concentrations from the reaction of red wine or distilled alcoholic drinks with physiological amounts of nitr...


LDL Isolated from Plasma-Loaded Red Wine Procyanidins Resist Lipid Oxidation an...

Lourenço, Cátia F.; Gago, Bruno; Barbosa, Rui M.; Freitas, Víctor de; Laranjinha, João

Dietary phenolic compounds may act as antioxidants in vitro, but because of structural modifications during absorption, its role based on concentrations high enough to afford an antioxidant protection needs to be re-evaluated. We have explored the hypothesis that red wine procyanidins interact with low density lipoproteins (LDL) and that, at this location, the phenolic compounds efficiently protect LDL from oxi...


Red wine-dependent reduction of nitrite to nitric oxide in the stomach

Gago, Bruno; Lundberg, Jon O.; Barbosa, Rui M.; Laranjinha, João

Nitrite may be a source for nitric oxide (NO), particularly in highly acidic environments, such as the stomach. Diet products contribute also with reductants that dramatically increase the production of NO from nitrite. Red wine has been attributed health promoting properties largely on basis of the reductive antioxidant properties of its polyphenolic fraction. We show in vitro that wine, wine anthocyanin fract...


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