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Measuring DNA modifications with the comet assay: a compendium of protocols

Collins, Andrew; Moller, Peter; Gajski, Goran; Vodenkova, Sona; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, Stefano

The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there ...

Date: 2023   |   Origin: RepositóriUM - Universidade do Minho
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