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Melatonin production improves Senegalese sole sperm motility at night, but fail...

Félix de Azeredo Pinto e Melo, Francisca; Ferrão, Leonor; Gallego, Victor; Oliveira, Catarina; Cabrita, Elsa

Melatonin is a powerful antioxidant present in fish seminal plasma. This study aimed to understand melatonin's endogenous and exogenous effects on first-generation Senegalese sole sperm quality for sperm management applications. In the first experiment, samples were collected at mid-light (ML) and mid-dark (MD) daytimes, to evaluate the effects on sperm motility. In a second experiment, using confocal microscop...


Cutting-Edge methods in Teleost and Chondrichthyan reproductive biology

Mechaly, Alejandro S.; Awruch, Cynthia; Cabrita, Elsa; Costas, Benjamín; Fernandes, Jorge M.O.; Gallego, Victor; Hirt-Chabbert, Jorge

Reproduction is a fundamental biological process for producing offspring and ensuring the continuation of the species. With nearly 35,000 fish species worldwide exhibiting a very diverse range of reproductive systems and strategies, the study of fish reproduction is a significant challenge. Research into various fish reproductive models is driven by their use in human biomedical applications, improving the dive...


Assessment of male reproductive traits in endangered leuciscids from the Iberia...

Hernández-Rodríguez, Ana; Sousa-Santos, Carla; Gil, Fátima; Cabrita, Elsa; Guerreiro, Pedro M; Gallego, Victor

During the spring of 2022, several endangered leuciscid species (Anaecypris hispanica, Squalius aradensis, Anachondrostoma Occidentale, and Iberochondrostoma lusitanicum) were sampled both at the Vasco da Gama aquarium facilities and in some rivers of the Algarve region, Portugal. Sperm samples were extracted by gentle abdominal pressure and sperm motion parameters were assessed for the frst time in four specie...


Assessment of male reproductive traits in endangered leuciscids from the Iberia...

Hernandez, Ana; Sousa-Santos, Carla; Gil, Fátima; Cabrita, Elsa; Guerreiro, Pedro; Gallego, Victor

During the spring of 2022, several endangered leuciscid species (Anaecypris hispanica, Squalius aradensis, Anachondrostoma Occidentale, and Iberochondrostoma lusitanicum) were sampled both at the Vasco da Gama aquarium facilities and in some rivers of the Algarve region, Portugal. Sperm samples were extracted by gentle abdominal pressure and sperm motion parameters were assessed for the first time in four speci...


Exposure to silver and titanium dioxide nanoparticles at supra-environmental co...

Oliveira, Catarina; Ferrão, Leonor; Gallego, Victor; Mieiro, Cláudia; Oliveira, Isabel B.; Carvalhais, Ana; Pachedo, Mário; Cabrita, Elsa

Marine pollution by nanoparticles (NPs) can be reprotoxic for fish and disturb successful reproduction of wild populations. In gilthead seabream (Sparus aurata), a mild effect on sperm motility was observed after exposure to high concentrations of silver NPs. Considering the great heterogeneity traits within a sperm sample, it is possible that NPs affect spermatozoa accordingly, modulating subpopulation profile...


Novel approaches on melatonin role: presence of clock-hormone in fish seminal p...

Félix, Francisca; Gallego, Victor; Mendes, Ana; Soares, Florbela; Vera, Luisa M.; Cabrita, Elsa; Oliveira, Catarina

The study of melatonin is of great importance for the fundamental knowledge of any living system since it displays many different physiological roles, including being a potent natural antioxidant. To the best of our knowledge, there is no information regarding melatonin in fish seminal plasma. This study aimed to determine this clock-hormone levels in the seminal plasma of three aquaculture fish species: Europe...


The importance of developing and Standardizing Gamete, Embryo and Larvae Handli...

Paredes, Estefanía; Gallego, Victor

Artificial reproduction in aquatic animals usually involves the collection and handling of gametes both from males and females in a way that secures their quality and optimizes the fertilization event. There are innumerable specie- and family-specific protocols among the various aquatic species that inhabit bodies of water, from freshwater lagoons to seawater oceans. In that sense, this Special Issue aims to ga...


Sperm handling in aquatic animals for artificial reproduction

Beirão, José; Boulais, Myrina; Gallego, Victor; O'Brien, Justine K.; Peixoto, Silvio; Robeck, Todd R.; Cabrita, Elsa

Artificial reproduction involves collection and handling of gametes in a way that secures their quality and maximizes the fertilization outcome. In addition to initial sperm quality, numerous steps can affect the final result of fertilization, from the sperm collection process until gamete mixing (or co-incubation) when the spermatozoon enters or fuses with the oocyte. In this review, we summarize the whole pro...


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