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Polysaccharide-mediated stabilization of bacterial nanocellulose dispersions: E...

Silva-Carvalho, Ricardo; Martins, Daniela Sofia Rodrigues; Castro, Tarsila Gabriel; Rodrigues, Ana Cristina; Costa, Bruna; Parreira, Paula

Colloidal dispersions of pristine cellulose (nano- or microsized; fibrillar or particulate), are typically unstable in water due to interfiber/interparticle interactions. Carboxymethyl cellulose (CMC) is known to effectively stabilize these dispersions, allowing for full recovery of the properties upon redispersion after drying. However, the underlying mechanism has been unclear. This study investigated why CMC...


Nanobubble-enhanced oxygen transfer in bacterial nanocellulose production: comp...

Rodrigues, Ana Cristina Costa; Martins, Daniela Sofia Rodrigues; Carvalho, Ricardo Emanuel Silva; Marques, Susana Cristina Gomes; Belo, Isabel

Despite the unique properties of bacterial nanocellulose (BNC), oxygen limitation during large-scale production impairs microbial metabolism and cellulose synthesis, leading to high production costs and limited commercial success. Static fermentation can achieve high titers, but industrially it is operationally challenging. Agitated systems like airlift (AL) bioreactors, allow faster production but typically yi...


Development of antimicrobial peptide-loaded hydrogels as potential scaffolds fo...

Noohi, Parisa; Abdekhodaie, Mohammad J.; Nekoofar, Mohammad H.; Gama, F. M.; Saadatmand, Maryam; Dummer, Paul M. H.

Introduction: As a recently developed treatment modality, regeneration of the pulp-dentin-like complex via tissue engineering approaches is the focus of considerable attention. The persistence of bacteria within the root canal spaces is well known as the major challenge of dental pulp regeneration. Antimicrobial peptides (AMPs) are short peptides with inhibitory activities against a broad spectrum of microorgan...


Optimizing bacterial nanocellulose production from eucalyptus bark: a circular ...

Rodrigues, Ana Cristina Costa; Martins, Daniela Sofia Rodrigues; Duarte, Maria Salomé Lira; Carvalho, Ricardo Emanuel Silva; Marques, Susana

The production cost of bacterial nanocellulose (BNC) is a major limitation to its widespread use. However, this limitation can be addressed by using alternative low-cost substrates and high-yield strains. Agro-industrial waste-derived substrates offer a cost-effective and sustainable solution, but their high organic load often requires additional downstream wastewater treatments. Here, we optimized static BNC p...


Life cycle assessment of bacterial cellulose and comparison to other cellulosic...

Soares da Silva, F. A. G.; Branco, Sara; Dourado, Fernando; Neto, Belmira; Gama, F. M.

Bacterial cellulose (BC) is a 3D exopolysaccharide synthesized by certain acetic acid bacteria, possessing unique properties such as nanofibrillar morphology and high purity. BC has gained increasing attention for several potential market applications, including in textiles. However, along with techno-economic challenges, the industrialization of BC pulp must align with sustainable practices and minimize the en...


On the microstructural anisotropy and mechanical properties of bacterial nanoce...

Costa, Lígia; Carvalho, Alexandre F.; Fernandes, António J. S.; Campos, Teresa; Dourado, Nuno; Rodrigues, Ana Cristina; Sampaio, Paula

The microstructural organization of bacterial nanocellulose is still not completely understood. Although its morphological and mechanical anisotropy is commonly recognized, it remains unclear whether the forces stabilizing the cellulose fiber network are comparable in different planes. In this study, we characterized the microstructure of statically produced BNC according to its horizontal and vertical planes. ...


Upcycling of cellulosic textile waste with bacterial cellulose via Ioncell® tec...

Schlapp-Hackl, Inge; Nygren, Nicole; Heimala, Senni; Leinonen, Anna; Dourado, Fernando; Gama, F. M.; Hummel, Michael; Silva, Francisco A.G.S.

Currently the textile industry relies strongly on synthetic fibres and cotton, which contribute to many environmental problems. Man-made cellulosic fibres (MMCF) can offer sustainable alternatives. Herein, the development of Lyocell-type MMCF using bacterial cellulose (BC) as alternative raw material in the Ioncell® spinning process was investigated. BC, known for its high degree of polymerization (DP), crystal...


Enhanced bacterial cellulose production in komagataeibacter sucrofermentans: im...

Silva, Pedro Moreira Montenegro Baptista; Ellis, Tom; Dourado, Fernando; Gama, F. M.; Domingues, Lucília

Background Bacterial cellulose (BC) is a biocompatible material with unique mechanical properties, thus holding a significant industrial potential. Despite many acetic acid bacteria (AAB) being BC overproducers, cost-effective production remains a challenge. The role of pyrroloquinoline quinone (PQQ)-dependent membrane dehydrogenases (mDH) is crucial in the metabolism of AAB since it links substrate incomplete ...


Laser‑patterning bacterial nanocellulose for cell‑controlled interaction

Costa, Lígia; Carvalho, Alexandre F.; Carvalho, Ricardo Emanuel Silva; Rodrigues, Ana Cristina; Dourado, Fernando; Deuermeier, Jonas; Neto, Miguel A.

The interfacial topography of biomaterials has been identified as a major biophysical regulator of cell behavior and function, a role played through the interplay with biochemical cues. In this work, we demonstrate the potential of laser as a versatile technology for the direct fine-tuning of the topography of Bacterial nanocellulose (BNC) with bioinspired topographies and micropatterns on a cell size scale. Tw...


Bacterial cellulose in vitro uptake by macrophages, epithelial cells, and a tri...

Carvalho, Ricardo Emanuel Silva; Rodrigues, Patrícia M.; Martins, Daniela Sofia Rodrigues; Rodrigues, Ana Cristina Costa; Sampaio, Paula

Bacterial cellulose (BC) has a long-standing human consumption history in different geographies without any report of adverse effects. Despite its unique textural and functional properties, the use of BC in food products in Europe is still restricted due to concerns over its nanosize. Here, we evaluated the potential uptake of celluloses (from plant and microbial sources, processed using different blenders) by ...


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