34 documents found, page 1 of 4
The impact of genetic groups (Alentejano and F1 Landrace x Large White pigs) an...
Amaral, Andreia; Cordeiro, Daniela; Bettencourt, Carlos; Moreira, Olga; Bressan, Maria Cristina; Belo, Ana Teresa; Boari, Cleube AndradeThis research work was carried out with the goal of studying the impact of genetic groups-GG (Alentejano-AL, n = 30, and F1 Landrace*Large White-F1 pigs, n = 30) and body weight-BW (90, 120 and 160 kg) on plasma metabolites. Blood parameters were correlated with animal production traits, carcass measurements and meat quality. Individual records for feed conversion index (CI) and daily feed intake were recorded ...
Human and companion animal proteus mirabilis sharing
Marques, Cátia Filipa Saraiva; Belas, Adriana; Menezes, Juliana; da Silva, Joana Moreira; Cavaco-Silva, Patrícia; Trigueiro, Graça; Gama, LuisProteus mirabilis is an important pathogen that is associated with urinary tract infections. This study aims to determine the colonization and sharing of P. mirabilis between healthy companion animals and humans that are living together and to evaluate the clonal relatedness of the fecal and clinical stains. Eighteen households (24 humans, 18 dogs, 8 cats) with at least one human–animal pair were studied. Fecal...
Generation of a library of carbohydrate-active enzymes for plant biomass decons...
Cardoso, Vânia; Bras, Joana L. A.; Costa, Ines F.; Ferreira, Luis M. A.; Gama, Luis; Vincentelli, Renaud; Henrissat, Bernard; Fontes, Carlos M.G.A.In nature, the deconstruction of plant carbohydrates is carried out by carbohydrate-active enzymes (CAZymes). A high-throughput (HTP) strategy was used to isolate and clone 1476 genes obtained from a diverse library of recombinant CAZymes covering a variety of sequence-based families, enzyme classes, and source organisms. All genes were successfully isolated by either PCR (61%) or gene synthesis (GS) (39%) and ...
Assessment of biofilm formation by Campylobacter spp. isolates mimicking poultr...
Araújo, P. M.; Batista, E.; Fernandes, M. H.; Fernandes, M. J.; Gama, Luis; Fraqueza, M. J.This research aimed to assess the biofilm formation ability of Campylobacter strains under temperature and oxygen stress conditions, similar to those found in the industrial environment, to explain the persistence of this pathogen on the poultry slaughter line. A collection of C. jejuni and C. coli isolates (n = 143) obtained from poultry samples (cecal content and neck skin), collected at slaughterhouse level,...
Myeloperoxidase inhibition decreases the expression of collagen and metallopept...
Amaral, Ana; Fernandes, Carina; Rebordão, Maria Rosa; Szostek-Mioduchowska, Anna; Lukasik, Karolina; Pinto-Bravo, Pedro; Gama, LuisABSTRACT - Neutrophils can originate neutrophil extracellular traps (NETs). Myeloperoxidase (MPO) is a peroxidase found in NETs associated to equine endometrosis and can be inhibited by 4-aminobenzoic acid hydrazide (ABAH). Metallopeptidases (MMPs) participate in extracellular matrix stability and fibrosis development. The objectives of this in vitro work were to investigate, in explants of mare’s endometrium, ...
Conditioning factors of linearized wood's function lactation curve shape parame...
Delgado Bermejo, Juan Vicente; Limón Pérez, Francisco Antonio; Navas González, Francisco Javier; León Jurado, Jose Manuel; Fernández Álvarez, JavierA total of 137,927 controls of 22,932 Murciano-Granadina first lactation goats (measured between 1996–2016) were evaluated to determine the influence of the number of kids, season, year and farm on total milk yield, daily milk yield, lactation length, total production of fat and protein and percentages of fat and protein. All factors analyzed had a significant effect on the variables studied, except for the inf...
Effect of high hydrostatic pressure challenge on biogenic amines, microbiota, a...
Borges, Ana F.; Cozar, Almudena; Patarata, Luis; Gama, Luis; Alfaia, Cristina; Fernandes, Maria J.; Fernandes, Maria H.; Perez Vergara, HerminiaThe processing of traditional poultry- and pork-based semidried fermented smoked sausages needs to bemodernized to improve product quality and further extend its shelf life. The aim of the present study was to applydifferent combinations of high pressure (300 to 600 MPa) and time (154 to 1,800 s) on the sausages using an experimentaldesign based on response surface methodology. The chemical, microbial, and sens...
Genomic tools for the conservation and genetic improvement of a highly fragment...
Amaral, A J; Pavao, Ana L.; Gama, LuisRamo Grande is a local cattle breed raised in the archipelago of Azores, with a small and dispersed census, where inbreeding control is of utmost importance. A single nucleotide polymorphism (SNP) Beadchip array was used to assess inbreeding, by analysis of genomic regions harboring contiguous homozygous genotypes named runs of homozygosity (ROH), and to estimate past e ective population size by analysis of lin...
The In vitro inhibitory effect of sivelestat on elastase induced collagen and m...
Amaral, Ana; Fernandes, Carina; Rebordão, Maria Rosa; Szóstek-Mioduchowska, Anna; Lukasik, Karolina; Gawronska-Kozak, Barbara; Gama, LuisAbstract: Neutrophil extracellular traps (NETs) fight endometritis, and elastase (ELA), a protease found in NETs, might induce collagen type I (COL1) accumulation in equine endometrium. Metallopeptidases (MMPs) are involved in extracellular matrix balance. The aim was to evaluate the e ects of ELA and sivelestat (selective elastase inhibitor) on MMP-2 and MMP-9 expression and gelatinolytic activity, as well as ...
Discrimination of meat produced by Bos taurus and Bos indicus finished under an...
Bressan, Maria C.; Rodrigues, Erika C.; Rossato, Lizandra V.; Neto-Fonseca, M. Isabel; Alves, Susana P.; Bessa, R.J.B.; Gama, LuisMeat obtained under commercial conditions shows considerable variability, mostly due to genetic background and production system. In this study, meat physicochemical properties and fatty acid profiles were analysed to investigate the feasibility of using them as tools to discriminate between meats produced by different genetic groups and finishing systems. Samples of the Longissimus thoracis were collected from...