Made available in DSpace on 2018-11-26T15:28:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-12-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation...
Since the first work about embryo collection, a great amount of research and development has founded on enhancing genetic contribution of cows. The aim of this publication is to give an account of the current knowledge, new findings and techniques on recovery of oocytes from cattle donors.; Desde o primeiro relato de colheita de embriões, um grande número de trabalhos tem apresentado os esforços para melhorar a...
The conditions of in vitro maturation of bovine oocytes (Exp. I) and bull effect (Exp. II) on cleavage (CR) and the in vitro morulae and blastocysts (MO/BL) rates were evaluated. Imatured oocytes were matured in vitro under different conditions: B-199 medium with 5% estrous cow serum (ECS), 5% fetal calf serum (FCS) and granulosa cells (T1/Exp. I) or B-199 medium with 10% FCS, 14 IU/ml PMSG and 7 IU/ml hCG (T2/...
Foram estudados 14 animais da espécie canina, sem raça definida, machos, adultos, com o objetivo de analisar, laboratorialmente, o sêmen e os aspectos histológicos dos testículos antes e após a administração do sulfato de vincristina. Para tanto, os animais foram divididos em 2 grupos. No grupo I (8 animais), submeteu-se os animais à orquiectomia unilateral e um fragmento do testículo e epidídimo foram analisad...