4 documents found, page 1 of 1
Usefulness of microchip electrophoresis for the analysis of mitochondrial DNA i...
Alonso, A; Albarrán, C; Martín, P; Garcia, P; Capilla, J; Garcia, O; Rua, C; Izaguirre, N; Pereira, F; Pereira, L; Amorim, A; Sancho, MWe evaluate the usefulness of a commercially available microchip CE (MCE) device in different genetic identification studies performed with mitochondrial DNA (mtDNA) targets, including the haplotype analysis of HVR1 and HVR2 and the study of interspecies diversity of cytochrome b (Cyt b) and 16S ribosomal RNA (16S rRNA) mitochondrial genes in forensic and ancient DNA samples. The MCE commercial system tested in...
Evaluating the forensic informativeness of mtDNA haplogroup H sub-typing on a E...
Pereira, L; Richards, M; Goios, A; Alonso, A; Albarrán, C; Garcia, O; Behar, DM; Gölge, M; Hatina, J; Al-Gazali, L; Bradley, DG; Macaulay, V; Amorim, AThe impact of phylogeographic information on mtDNA forensics has been limited to the quality control of published sequences and databases. In this work we use the information already available on Eurasian mtDNA phylogeography to guide the choice of coding-region SNPs for haplogroup H. This sub-typing is particularly important in forensics since, even when sequencing both HVRI and HVRII, the discriminating power...
Mitochondrial DNA haplotyping revealed the presence of mixed up benign and neop...
Alonso, A; Alves, C; Suárez-Mier, MP; Albarrán, C; Pereira, L; Fernandez de Simón, LF; Martín, P; Garcia, O; Gusmão, L; Sancho, M; Amorim, ADNA typing was requested to investigate a presumptive cancer diagnosis error by confirming whether benign and cancerous prostatic tissue in the same presurgical haematoxylin and eosin stained slide belonged to the same person. After independent histological re-examination of the slide by a pathologist, manual slide dissection was used to guarantee independent and high recovery DNA isolation from each tissue sec...
High-resolution mtDNA evidence for the late-glacial resettlement of Europe from...
Pereira, L; Richards, M; Goios, A; Alonso, A; Albarrán, C; Garcia, O; Behar, DM; Gölge, M; Hatina, J; Al-Gazali, L; Bradley, DG; Macaulay, V; Amorim, AThe advent of complete mitochondrial DNA (mtDNA) sequence data has ushered in a new phase of human evolutionary studies. Even quite limited volumes of complete mtDNA sequence data can now be used to identify the critical polymorphisms that define sub-clades within an mtDNA haplogroup, providing a springboard for large-scale high-resolution screening of human mtDNAs. This strategy has in the past been applied to...