5 documents found, page 1 of 1
Combining the Pharmacophore Features of Coumarins and 1,4-Substituted 1,2,3-Tri...
Torres,Fernando C.; Gonçalves,Guilherme A.; Vanzolini,Kenia L.; Merlo,Aloir A.; Gauer,Bruna; Holzschuh,Maribete; Andrade,Saulo; Piedade,MaristelaCoumarins are a large class of compounds that display a range of interesting biological properties, being considered privileged structures because of the ability of their 2H-chromen-2-one nuclei to bind to multiple pharmacological targets. We hypothesized that the linkage of a second pharmacophore nucleus to the 2H-chromen-2-one core, the 1,2,3-triazole moiety, would entail more selective and pharmacologically ...
Simultaneous quantification of lycopene, β-carotene, retinol and α -tocopherol ...
Charão,Mariele F.; Moro,Angela M.; Brucker,Natália; Bulcão,Rachel P.; Baierle,Marília; Freitas,Fernando; Durgante,Juliano; Nascimento,SabrinaA method for the simultaneous quantification of lycopene, β-carotene, retinol and α-tocopherol by high-performance liquid chromatography (HPLC) with Vis/fluorescence detection with isocratic elution was optimized and validated. The method consists of a rapid and simple liquid-liquid extraction procedure and a posterior quantification of extracted supernatants by HPLC. Aliquots of plasma were stored at -20°C for...
Determination of metallic contaminants in human recombinant erythropoietin (EPO...
Carvalho,Leandro M. de; Garmatz,Júlia C.; Spengler,Cristiane; Nascimento,Paulo C. do; Bohrer,Denise; Canabarro,Cibele M.; Garcia,Solange C.The contamination of renal failure patients by metals can be associated to the presence of toxic metals in the medication, since they can be absorbed by the organism. Among the possible contaminants, there are nephrotoxic metals such as aluminum, chromium and nickel. In the present work, the presence of Al, Cr and Ni as contaminants in erythropoietin formulations (EPO) was investigated by optimizing the adsorpt...
Determinação voltamétrica de metais em águas e fluidos biológicos empregando mi...
Carvalho,Leandro M. de; Spengler,Cristiane; Garmatz,Júlia C.; Nascimento,Paulo C. do; Bohrer,Denise; Del-Fabro,Luciana; Radis,GuilhermeThis work describes the optimization of pretreatment steps for the destruction of organic matter in samples of waters and biological fluids, by using an UV irradiation system with a high power UV radiation source (400 W). The efficiency of the system constructed for the photo-decomposition of samples of model waters, natural waters and biological fluids was investigated by performing recovery experiments of the...
Influência de desproteinizantes ácidos na quantificação da glutationa reduzida ...
Schott,Karen L.; Charão,Mariele F.; Valentin,Juliana; Cassol,Juniara; Garcia,Solange C.; Pomblum,Valdeci J.; Bohrer,DeniseLarge differences in reduced glutathione (GSH) levels have been found in different investigations, also in healthy people. GSH oxidation in vitro has been associated with sample acidification in the presence of oxihemoglobin. In this work, the influence of different acids on GSH determination utilizing HPLC with UV detection was evaluated. The results showed that metaphosphoric acid and sulfosalicylic acid were...