9 documents found, page 1 of 1
Evaluation of the Suitability of Analytical Methods in Trichothecene A and B De...
Feltrin,Ana Carla P.; Sibaja,Karen V. M.; Tusnski,Cíntia; Caldas,Sergiane S.; Primel,Ednei G.; Garda-Buffon,JaquelineAn investigation was conducted to standardize conditions for laccase and lipase activity required to maximize trichothecene degradation. The analytical methods were suitable and validated in model solutions for detection of five analytes (T-2 toxin, deoxynivalenol, nivalenol, 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol), the methods were then applied in the degradation tests of these trichothecenes. The ...
PEROXIDASE FROM SOYBEAN MEAL: OBTENTION, PURIFICATION AND APPLICATION IN REDUCT...
Feltrin,Ana Carla Penteado; Fontes,Milena Ramos Vaz; Gracia,Henrique Delgado Kikumoto; Badiale-Furlong,Eliana; Garda-Buffon,JaquelineThis study established the conditions for the extraction of the enzyme peroxidase (PO) from soybean meal (SBM). An experimental design methodology was carried out in order to evaluate the effects of stirring rate time, pH and extracting solvent volume on the enzyme extraction. By using 5 g SBM and 50 mL phosphate buffer 10 mmol L-1 pH 4.7, 60 min stirring rate at 100 rpm, an enzyme with specific activity of 100...
Fusarium graminearum growth inhibition mechanism using phenolic compounds from ...
Pagnussatt,Fernanda Arnhold; Kupski,Larine; Darley,Francine Toralles; Filoda,Paula Freitas; Ponte,Émerson Medeiros Del; Garda-Buffon,JaquelineThe application of natural antifungal substances is motivated by the need for alternatives to existing methods that are not always applicable, efficient, or that do not pose risk to consumers or the environment. Furthermore, studies on the behaviour of toxigenic species in the presence of natural fungicides have enabled their safe application in the food chain In this study, Spirulina LEB-18 phenolic extract wa...
Simultaneous extraction and detection of ochratoxin A and citrinin in rice
Hackbart,Helen C. S.; Prietto,Luciana; Primel,Ednei G.; Garda-Buffon,Jaqueline; Badiale-Furlong,ElianaThis study compared four extraction procedures for the simultaneous determination of mycotoxins ochratoxin A and citrinin in samples of rice. Soares and Rodriguez-Amaya and Tanaka procedures and the extraction methods QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) and by ultrasound were compared in terms of residue generation, limit of detection, limit of quantification and simultaneous recovery of m...
Método QuEChERS para determinação de ocratoxina a e citrinina em arroz e farelo...
Hackbart,Helen C. dos S.; Souza,Michele Moraes de; Scaglioni,Priscila Tessmer; Primel,Ednei G.; Garda-Buffon,Jaqueline; Badiale-Furlong,ElianaThe present study was carried out to establish the optimal conditions for performing ochratoxin A (OTA) and citrinin (CIT) extraction using the QuEChERS method in rice. Employing the factorial experimental design, variables that significantly influenced the extraction stages were determined. The following variables were analyzed: addition of water, acidification of acetonitrile with glacial acetic acid, as well...
Análises quali e quantitativas de micotoxinas em águas da cadeia produtiva do a...
Leite,Clarice Caldeira; Garda-Buffon,Jaqueline; Fagundes,Carlos Alberto; Badiale-Furlong,ElianaThis study validated a simple and applied method for determining mycotoxins aflatoxin B1, aflatoxin B2, ochratoxin A, zearalenone and deoxynivalenol, in water from the rice production chain. Five solvent combinations for extraction were tested, with quantification performed by TLC/HPTLC and confirmation by LC-MS/MS. Mycotoxins in water from field and rice industries were evaluated. Mycotoxin recovery levels wer...
Deoxynivalenol (DON) degradation and peroxidase enzyme activity in submerged fe...
Garda-Buffon,Jaqueline; Kupski,Larine; Badiale-Furlong,ElianaThis work aims to evaluate deoxynivalenol degradation by Aspergillus oryzae and Rhizopus oryzae in a submerged fermentation system and to correlate it to the activity of oxydo-reductase enzymes. The submerged medium consisted of sterile distilled water contaminated with 50 μg of DON and 4 × 10(6) spore.mL-1 inoculum of Aspergillus oryzae and Rhizopus oryzae species, respectively in each experiment. Sampling was...
Effect of deoxynivalenol and T-2 toxin in malt amylase activity
Garda-Buffon,Jaqueline; Baraj,Edlira; Badiale-Furlong,ElianaThe objective of this work was to evaluate the effect of DON and T-2 toxin levels on the malt amylase activity. The malt was contaminated in accordance with central composite design experiment with DON and T-2 toxin levels until 1000 ng/g. The activities of the enzymes were evaluated by Bernfeld method. The increase in T-2 toxin concentration inhibited the α-amylase activity. However, the increase of both toxin...
Kinetics of deoxynivalenol degradation by Aspergillus oryzae and Rhizopus oryza...
Garda-Buffon,Jaqueline; Badiale-Furlong,ElianaThe objective was to evaluate the capacity of Aspergillus oryzae and Rhizopus oryzae to degrade deoxynivalenol (DON) during submerged fermentation. The submerged medium utilized was sterile distilled water with 1μg mL-1 DON, added and inoculated with 4 × 10(6) spores mL-1 of the fungal species. Sampling was performed every 48 h to 240 h. DON analyses included residual mass, percentage and velocity of degradatio...