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Probe-based metagenomic pathogen detection: advancing laboratory capacity for c...

Ferreira, Rita; Coelho, Luís; Santos, João Dourado; Sobral, Daniel; Isidro, Joana; Mixão, Verónica; Pinto, Miguel; Nunes, Alexandra; Borrego, Maria José

Probe-based pathogen enrichment, followed by NGS, is a promising tool for complex diagnosis, overcoming traditional challenges of shotgun metagenomics, namely small microbial/human genetic material ratio and demanding computational resources. Here, we assessed the combined detection performance of two Illumina probe-based panels, the Respiratory and the Urinary Pathogen ID panels (RPIP and UPIP), using 99 clini...


Rede de Vigilância de Vetores - Flebótomos: a importância da vigilância de um v...

Amaro, Fátima; Osório, Hugo; Silva, Manuel; Freitas, Inês Campos; Soares, Patrícia; Vilares, Anabela; Martins, Susana; Reis, Tânia; Gargaté, Maria João

O programa REVIVE (Rede de Vigilância de Vetores) resulta de colaboração entre a Direção-Geral da Saúde, as Administrações Regionais de Saúde do Algarve, Alentejo, Centro, Lisboa e Vale do Tejo e Norte, a Direção Regional da Saúde da Madeira, a Direção Regional da Saúde dos Açores e o Instituto Nacional de Saúde Doutor Ricardo Jorge. No âmbito do REVIVE é realizada a vigilância entomológica, a nível nacional, d...


Toxocaríase: confirmação laboratorial de casos clínicos suspeitos de infeção po...

Reis, Tânia; Vilares, Anabela; Martins, Susana; Ferreira, Idalina; Gargaté, Maria João

A toxocaríase é uma infeção parasitária zoonótica com distribuição mundial, considerada pelo Centre for Disease Control and Prevention (CDC) uma das doenças parasitárias mais negligenciadas e que apresenta uma taxa de prevalência subestimada, sendo raros os estudos sobre a seroprevalência para Toxocara spp. em Portugal. A infeção no Homem pode ocorrer de várias formas, sendo predominante a infeção através da in...


Co-Circulation of Leishmania Parasites and Phleboviruses in a Population of San...

Amaro, Fátima; Vilares, Anabela; Martins, Susana; Reis, Tânia; Osório, Hugo Costa; Alves, Maria João; Gargaté, Maria João

In the Old World, phlebotomine sand flies from the genus Phlebotomus are implicated in the transmission of Leishmania spp. parasites (Kinetoplastida: Trypanosomatidae) and viruses belonging to the genus Phlebovirus (Bunyavirales: Phenuiviridae). Two of the five sand fly species known to occur in Portugal, Phlebotomus perniciosus and Ph. ariasi, the former being the most ubiquitous, are recognized vectors of Lei...


Unveiling the incidences and trends of the neglected zoonosis cystic echinococc...

Casulli, Adriano; Abela-Ridder, Bernadette; Petrone, Daniele; Fabiani, Massimo; Bobić, Branko; Carmena, David; Šoba, Barbara; Zerem, Enver

The neglected zoonosis cystic echinococcosis affects mainly pastoral and rural communities in both low-income and upper-middle-income countries. In Europe, it should be regarded as an orphan and rare disease. Although human cystic echinococcosis is a notifiable parasitic infectious disease in most European countries, in practice it is largely under-reported by national health systems. To fill this gap, we extra...


Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Mo...

Gargaté, Maria João; Vilares, Anabela; Ferreira, Idalina; Reis, Tânia; Martins, Susana; Mendonça, Joana; Borges, Vítor; Gomes, João Paulo

Toxoplasma gondii is an obligate intracellular protozoan. In pregnant women, it can lead to severe birth defects or intrauterine death of the fetus. Most of what is currently know on cell biology of T. gondii comes from studies relying on the RH strain propagated in mice. According to the recommendations concerning the animal welfare, we assayed in vitro/in vivo procedures to replace, or at least reduce, the de...


First report of Echinococcus ortleppi in free-living wild boar (Sus scrofa) fro...

Mateus, Teresa Letra; Gargaté, Maria João; Vilares, Anabela; Ferreira, Idalina; Rodrigues, Manuela; Coelho, Catarina; Vieira-Pinto, Madalena

Cystic echinococcosis (CE) is a zoonosis that is prevalent worldwide. It is considered endemic in Portugal but few studies have been performed on Echinococcus granulosus sensu lato and their hosts. In this study, CE cysts are reported for the first time in a free-living wild boar (Sus scrofa) in Portugal. The presence of the metacestodes in the liver of the wild boar was identified by morphological features, mi...

Date: 2021   |   Origin: Repositório Científico IPVC

First Report of Echinococcus ortleppi in Free-Living Wild Boar (Sus scrofa) fro...

Mateus, Teresa Letra; Gargaté, Maria João; Vilares, Anabela; Ferreira, Idalina; Rodrigues, Manuela; Coelho, Catarina; Vieira-Pinto, Madalena

Cystic echinococcosis (CE) is a zoonosis that is prevalent worldwide. It is considered endemic in Portugal but few studies have been performed on Echinococcus granulosus sensu lato and their hosts. In this study, CE cysts are reported for the first time in a free-living wild boar (Sus scrofa) in Portugal. The presence of the metacestodes in the liver of the wild boar was identified by morphological features, mi...


First Report of Echinococcus ortleppi in Free-Living Wild Boar (Sus scrofa) fro...

Mateus, Teresa Letra; Gargaté, Maria João; Vilares, Anabela; Ferreira, Idalina; Rodrigues, Manuela; Coelho, Catarina; Vieira-Pinto, Madalena

Cystic echinococcosis (CE) is a zoonosis that is prevalent worldwide. It is considered endemic in Portugal but few studies have been performed on Echinococcus granulosus sensu lato and their hosts. In this study, CE cysts are reported for the first time in a free-living wild boar (Sus scrofa) in Portugal. The presence of the metacestodes in the liver of the wild boar was identified by morphological features, mi...


First Report of Echinococcus ortleppi in Free-Living Wild Boar (Sus scrofa) fro...

Mateus, Teresa Letra; Gargaté, Maria João; Vilares, Anabela; Ferreira, Idalina; Rodrigues, Manuela; Coelho, Catarina Manuela Almeida

Date: 2021   |   Origin: Repositório da UTAD

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