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Nano versus bulk: Evaluating the toxicity of lanthanum, yttrium, and cerium oxi...

Santos, Joana; Neca, Inês; Capitão, Ana; Nogueira, João; Santos, José A. S.; Pinto, Edgar; Barreto, Angela; Silva, Ana L. Daniel da; Maria, Vera L.

Considering the increase in demand for rare earth elements (REEs) and their accumulation in soil ecosystems, it is crucial to understand their toxicity. However, the impact of lanthanum, yttrium and cerium oxides (La2O3, Y2O3 and CeO2, respectively) on soil organisms remains insufficiently studied. This study aims to unravel the effects of La2O3, Y2O3 and CeO2 nanoparticles (NPs) and their corresponding bulk fo...


First approach to assess the effects of nanoplastics on the soil species Folsom...

Barreto, Ângela; Santos, Joana; Almeida, Lara; Tavares, Vítor; Pinto, Edgar; Celeiro, Maria; Garcia-Jares, Carmen; Maria, Vera L.

The terrestrial environment is one of the main recipients of plastic waste. However, limited research has been performed on soil contamination by plastics and even less assessing the effects of nanoplastics (NPls). Behind the potential toxicity caused per se, NPls are recognized vectors of other environmental harmful contaminants. Therefore, the main aim of the present study is to understand whether the toxicit...


A comprehensive ecotoxicity study of molybdenum disulfide nanosheets versus bul...

Santos, Joana; Barreto, Ângela; Fernandes, Cristiana; Silva, Ana Rita R.; Cardoso, Diogo N.; Pinto, Edgar; Daniel-da-Silva, Ana L.; Maria, Vera L.

The increasing use of molybdenum disulfide (MoS2) nanoparticles (NPs) raises concerns regarding their accumulation in soil ecosystems, with limited studies on their impact on soil organisms. Study aim: To unravel the effects of MoS2 nanosheets (two-dimensional (2D) MoS2 NPs) and bulk MoS2 (156, 313, 625, 1250, 2500 mg/kg) on Enchytraeus crypticus and Folsomia candida. The organisms’ survival and avoidance behav...


Toxicity of boron and vanadium nanoparticles on Danio rerio embryos - phenotypi...

Santos, Joana; Barreto, Ângela; Almeida, Célia; Azevedo, Cátia; Domingues, Inês; Amorim, Mónica J. B.; Maria, Vera L.

Engineered nanoparticles (NPs) are emerging contaminants of concern and it is important to understand their environmental behavior and ecological risks to exposed organisms. Despite their ubiquitous presence in the environment, there is little information about the hazards of certain NPs, such as boron (BNPs) and vanadium (VNPs). The aim of the present research was to investigate the effects of commercial BNPs ...


Transcriptomic effects of the non-steroidal anti-inflammatory drug Ibuprofen in...

Maria, Vera L.; Amorim, Monica J. B.; Bebianno, Maria João; Dondero, Francesco

The transcriptomic effects of Ibuprofen (IBU) in the digestive gland tissue of Mytilus galloprovincialis Lam. specimens exposed at low environmental concentrations (250 ng L-1) are presented. Using a 1.7 K feature cDNA microarray along with linear models and empirical Bayes statistical methods 225 differentially expressed genes were identified in mussels treated with IBU across a 15-day period. Transcriptional ...


Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at populat...

Mendes, Luís André; Maria, Vera L.; Scott-Fordsmand, Janeck J.; Amorim, Mónica J. B.

The effects of nanomaterials have been primarily assessed based on standard ecotoxicity guidelines. However, by adapting alternative measures the information gained could be enhanced considerably, e.g., studies should focus on more mechanistic approaches. Here, the environmental risk posed by the presence of silver nanoparticles (Ag NM300K) in soil was investigated, anchoring population and cellular level effec...


Oxidative stress mechanisms caused by ag nanoparticles (NM300K) are different f...

Ribeiro, Maria J.; Maria, Vera L.; Scott-Fordsmand, Janeck J.; Amorim, Mónica J. B.

The mechanisms of toxicity of Ag nanoparticles (NPs) are unclear, in particular in the terrestrial environment. In this study the effects of AgNP (AgNM300K) were assessed in terms of oxidative stress in the soil worm Enchytraeus crypticus, using a range of biochemical markers [catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR), total glutathione (TG), metal...


Oxidative stress biomarkers and metallothionein in Folsomia candida - responses...

Maria, Vera L.; Ribeiro, Maria João; Amorim, Mónica J. B.

Folsomia candida (Collembola) is a standard soil ecotoxicological species; effect assessment includes survival and reproduction as endpoints. In the present study, and for the first time, a range of oxidative stress biomarkers measurement was optimized and validated. The antioxidant capacity was measured by the activities of catalase (CAT), glutathione reductase (GR), glutathione-s-transferase (GST) and content...


Detection of emerging contaminants (UV filters, UV stabilizers and musks) in ma...

Picot Groz, M.; Martinez Bueno, M.J.; Rosain, D.; Fenet, H.; Casellas, C.; Pereira, Catarina; Maria, Vera L.; Bebianno, Maria João; Gomez, E.

The UV filters and musk fragrances have come into focus because these compounds are contained and increasingly used not only in sunscreen products but also in many products of daily use, such as cosmetics, skin creams, plastics or varnish. In view of this, the main objective of the present work was to develop and validate a method for the determination of three UV filters, two UV stabilizers and four musks in m...


Antioxidant and lipid peroxidation responses in Mytilus galloprovincialis expos...

Maria, Vera L.; Bebianno, Maria João

This study aimed to assess the antioxidant system potential and lipid peroxidative effects, in the gill and digestive gland of Mytilus galloprovincialis exposed to individual and binary mixtures of benzo(a)pyrene (BaP) and Cu for 7 days. Data demonstrated that in mussels exposed to BaP antioxidant enzymes (catalase--CAT, total glutathione peroxidase--tGPx, glutathione S-transferase--GST and glutathione reductas...


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