45 documents found, page 1 of 5
Gold nanoparticles: a promising next-generation tool for breast cancer photothe...
Ferreira-Gonçalves, Tânia; Costa, Eduardo; Coelho, João; Gaspar, Maria Manuela; Ascensão, Lia; Ferreira-Santos, Pedro; Faísca, Pedro; Figueiredo, IsabelAuNPs show suitable mean sizes and maximum absorbance peaks to be used for PTT when locally administered. AuNPs show no toxicity either in breast cancer cell lines or red blood cells. Laser irradiation proved to be safe when applied alone. Combining EGF-conjugated AuNPs with laser irradiation resulted in a reduction of 33% in MCF-7 cells viability. The animal model was successfully developed (all animals develo...
Locust bean gum (LBG) – a potential excipient for inhalation purposes: excipien...
Pontes, Jorge Filipe; Guerreiro, Filipa; da Silva, Joana Pinto; Almeida, Maria; Rosso, Annalisa; Rosa da Costa, Ana M; Agusti, GéraldineDry powders proposed for lung drug delivery typically involve excipients not approved for inhalation. The physicochemical characterisation of excipients is informative regarding their interaction with different body structures. Locust Bean Gum (LBG) has been proposed for diverse applications, including inhalation, benefiting from targeting ability towards phagocytic cells owing to the presence of mannose moieti...
Metabolism-Targeted Therapy in NSCLC - A New Theranostics Inhalation Approach U...
Mendes, Cindy; Martins, Filipa; Granja, Sara; Gonçalves, Joana; Barros, Hélio; Casimiro, Teresa; Aguiar-Ricardo, Ana; Silva, Fernanda; Abreu, BrunaLung cancer is one of the most lethal cancers globally, primarily due to delayed diagnosis and lack of specific and effective therapy. Increased lactate production and consumption, along with cysteine metabolic reliance, are features identified in NSCLC in our recent studies. Cancer metabolic remodeling leads to excessive ROS production, triggering oxidative stress, promoting angiogenesis, causing cellular and ...
In Vivo Measurements of Murine Melanoma's Complex Permittivity Between 500 MHz ...
Pelicano, Ana Catarina; Gaspar, Maria Manuela; Reis, Catarina P.; Araújo, Nuno A. M.; Conceição, Raquel C.; Godinho, Daniela M.Complex permittivity measurements of murine melanoma tumours were performed via open-ended coaxial probe technique for frequencies ranging from 500 MHz to 8.5 GHz. The measurements were conducted in five mice containing a melanoma tumour each, while the animals were under anaesthesia. Two different measurement methodologies were employed. Eighty-six in vivo measurements of melanoma’s permittivity were collected...
Antimicrobial activity of prophage endolysins against critical Enterobacteriace...
Gonçalves, Tiago; Marques, Andreia T.; Manageiro, Vera; Tanoeiro, Luis; Vital, Joana S.; Duarte, Aida; Vítor, Jorge M. B.; Caniça, ManuelaEnterobacteriaceae species are part of the 2017 World Health Organization antibiotic-resistant priority pathogens list for development of novel medicines. Multidrug-resistant Klebsiella pneumoniae is an increasing threat to public health and has become a relevant human pathogen involved in life-threatening infections. Phage therapy involves the use of phages or their lytic endolysins as bioagents for the treatm...
Antimicrobial activity of prophage endolysins against critical Enterobacteriace...
Gonçalves, Tiago; Marques, Andreia T.; Manageiro, Vera; Tanoeiro, Luis; Vital, Joana S.; Duarte, Aida; Vítor, Jorge M.B.; Caniça, ManuelaEnterobacteriaceae species are part of the 2017 World Health Organization antibiotic-resistant priority pathogens list for development of novel medicines. Multidrug-resistant Klebsiella pneumoniae is an increasing threat to public health and has become a relevant human pathogen involved in life-threatening infections. Phage therapy involves the use of phages or their lytic endolysins as bioagents for the treatm...
Liposomal delivery of repurposed antiviral drug saquinavir to macrophages as a ...
Mandal, Manoj; Pires, David; Pinho, Jacinta; Catalão, Maria João; Almeida, António José; Azevedo-Pereira, José Miguel; Gaspar, Maria Manuela; Anes, ElsaMycobacterium tuberculosis (Mtb) latently infects approximately a quarter of the world’s population and 10 % of these will develop the disease tuberculosis. Mtb infects macrophages, manipulating the proteolytic mechanisms, particularly, by decreasing the expression and activity of lysosomal cathepsins. Consequently, Mtb survives and even replicates inside macrophages concomitant with poor priming of the adaptiv...
Liposomal delivery of saquinavir to macrophages overcomes cathepsin blockade by...
Pires, David; Mandal, Manoj; Pinho, Jacinta; Catalão, Maria João; Almeida, António José; Azevedo-Pereira, José Miguel; Gaspar, Maria Manuela; Anes, ElsaMycobacterium tuberculosis is able to establish a chronic colonization of lung macrophages in a controlled replication manner, giving rise to a so-called latent infection. Conversely, when intracellular bacteria undergo actively uncontrolled replication rates, they provide the switch for the active infection called tuberculosis to occur. Our group found that the pathogen is able to manipulate the activity of en...
Liposomal Delivery of Saquinavir to Macrophages Overcomes Cathepsin Blockade by...
Pires, David; Mandal, Manoj; Pinho, Jacinta O.; Catalão, Maria João; Almeida, António J.; Azevedo-Pereira, J. M.; Gaspar, Maria Manuela; Anes, ElsaMycobacterium tuberculosis is able to establish a chronic colonization of lung macrophages in a controlled replication manner, giving rise to a so-called latent infection. Conversely, when intracellular bacteria undergo actively uncontrolled replication rates, they provide the switch for the active infection called tuberculosis to occur. Our group found that the pathogen is able to manipulate the activity of en...
Pluronic® F127 Hydrogel Containing Silver Nanoparticles in Skin Burn Regenerati...
Francisco, Pedro; Neves Amaral, Mariana; Neves, Afonso; Ferreira-Gonçalves, Tânia; Viana, Ana S.; Catarino, José; Faísca, Pedro; Simões, SandraPresently, skin burns are considered one of the main public health problems and lack therapeutic options. In recent years, silver nanoparticles (AgNPs) have been widely studied, playing an increasingly important role in wound healing due to their antibacterial activity. This work is focused on the production and characterization of AgNPs loaded in a Pluronic® F127 hydrogel, as well as assessing its antimicrobia...