37 documents found, page 1 of 4
In-bath 3D printing of anisotropic shape-memory cryogels functionalized with bo...
Castanheira, Edgar J.; Monteiro, Luís P. G.; Gaspar, Vítor M.; Correia, Tiago R.; Rodrigues, João M. M.; Mano, João F.Cryogels exhibit unique shape memory with full recovery and structural stability features after multiple injections. These constructs also possess enhanced cell permeability and nutrient diffusion when compared to typical bulk hydrogels. Volumetric processing of cryogels functionalized with nanosized units has potential to widen their biomedical applications, however this has remained challenging and relatively...
Advances in cell-rich inks for biofabricating living architectures
Almeida-Pinto, José; Moura, Beatriz S.; Gaspar, Vítor M.; Mano, João F.Advancing biofabrication toward manufacturing living constructs with well-defined architectures and increasingly biologically relevant cell densities is highly desired to mimic the biofunctionality of native human tissues. The formulation of tissue-like, cell-dense inks for biofabrication remains, however, challenging at various levels of the bioprinting process. Promising advances have been made toward this go...
Engineered nascent living human tissues with unit programmability
Lavrador, Pedro; Moura, Beatriz S.; Almeida-Pinto, José; Gaspar, Vítor M.; Mano, João F.Leveraging human cells as materials precursors is a promising approach for fabricating living materials with tissue-like functionalities and cellular programmability. Here we describe a set of cellular units with metabolically engineered glycoproteins that allow cells to tether together to function as macrotissue building blocks and bioeffectors. The generated human living materials, termed as Cellgels, can be ...
Emerging lipid-polymer hybrid nanoparticles for genome editing
Gameiro, Mariana; Mano, João F.; Gaspar, Vítor M.Genome editing technologies have been key to unlocking new bioengineering strategies as they enable the modification of mammalian cells’ genes in a fully user-programmed mode. Despite major advancements, the development of proficient systems for a safer and more efficient delivery of gene editing machineries into all classes of mammalian cells is still challenging. In this context, new generations of lipid–poly...
Double‐interlinked colloidal gels for programable fabrication of supraparticle ...
Gonçalves, Leandro; Lavrador, Pedro; Amaral, Adérito J. R.; Ferreira, Luís P.; Gaspar, Vítor M.; Mano, João F.Engineering colloidal gel inks with suitable features for fabricating robust supraparticle architectures through 3D printing may overcome the challenges of precisely controlling nanoparticles spatial distribution across multiple scales. Herein, oppositely charged proteinaceous-polymeric nanoparticles are combined to generate multi-component colloidal gel (COGEL) inks for fabricating supraparticle volumetric arc...
Programmable Granular Hydrogel Inks for 3D Bioprinting Applications
Ribeiro, Lucas S.; Gaspar, Vítor M.; Sobreiro Almeida, Rita; Camargo, Emerson R.; Mano, João F.Granular inks comprising jammed hydrogel unit building blocks are emerging as multiprogramable precursors for 3D/4D printing nonbulk hydrogel constructs. In addition to their injectability, they also exhibit high porosity when compared to bulk hydrogels, allowing more efficient nutrient transport and cell migration through the scaffold structure. Herein, the key steps in the production of these inks, from the f...
Photo‐compartmentalized decellularized matrix‐hyaluronan hybrid units for pancr...
Monteiro, Maria V.; Henriques-Pereira, Margarida; Neves, Bruno M.; Duarte, Iola D.; Gaspar, Vítor M.; Mano, João F.Tissue-derived decellularized extracellular matrix (dECM) provides unique biomolecular cues for bioengineering 3D tumor models with increasingly physiomimetic features. This biomimicry potential can be expanded by combining dECM with key components of native tissues, such as glycosaminoglycans (GAGs). Yet, this combination remains highly challenging, often requiring complex non-natural chemical modifications of...
Brewer's yeast polysaccharides: a review of their exquisite structural features...
Bastos, Rita; Oliveira, Patrícia G.; Gaspar, Vítor M.; Mano, João F.; Coimbra, Manuel A.; Coelho, ElisabeteRecent advances on brewer’s yeast cell wall polysaccharides have unraveled exquisite structural features and diverse composition with (β1→3), (β1→6), (α1→4), (β1→4)- mix-linked glucans that are recognized to interact with different cell receptors and trigger specific biological responses. Herein, a comprehensive showcase of structurebiofunctional relationships between yeast polysaccharides and their biological ...
Programmable living units for emulating pancreatic tumor-stroma interplay
Monteiro, Maria V.; Rocha, Marta; Gaspar, Vítor M.; Mano, João F.Bioengineering close-to-native in vitro models that emulate tumors bioarchitecture and microenvironment is highly appreciable for improving disease modeling toolboxes. Herein, pancreatic cancer living units-so termed cancer-on-a-bead models-are generated. Such user-programmable in vitro platforms exhibit biomimetic multicompartmentalization and tunable integration of cancer associated stromal elements. These st...
Advances in bioengineering pancreatic tumor-stroma physiomimetic Biomodels
Monteiro, Maria V.; Ferreira, Luís P.; Rocha, Marta; Gaspar, Vítor M.; Mano, João F.Pancreatic cancer exhibits a unique bioarchitecture and desmoplastic cancer-stoma interplay that governs disease progression, multi-resistance, and metastasis. Emulating the biological features and microenvironment heterogeneity of pancreatic cancer stroma in vitro is remarkably complex, yet highly desirable for advancing the discovery of innovative therapeutics. Diverse bioengineering approaches exploiting pat...