7 documents found, page 1 of 1
Stimuli-responsive Nanocomposite Hydrogels Incorporating Soft Nanoparticles for...
Lavrador, P.; Borges, J.; Gaspar, V. M.; Mano, J. F.Soft nanoparticles represent a unique class of nanomaterials that can be engineered to react and adapt distinctly in different biological milieus while modulating the presentation of biochemical and biophysical cues to neighbouring receptive cells. This flexibility has fuelled the development of soft nanoparticle-laden nanocomposite hydrogels that are increasingly sophisticated in stimuli-responsiveness and pro...
Chapter 25. Decellularized Matrix Hydrogels for In Vitro Disease Modeling
Ferreira, L. P.; Monteiro, M. V.; Gaspar, V. M.; Mano, J. F.The extracellular matrix (ECM) operates as a complex network of cell-supporting macromolecules in tissue homeostasis and disease scenarios. Given ECM structural and bio-signaling roles, understanding and modeling matrix components and their dysfunction in disease is crucial for the development of novel therapeutic approaches for numerous pathologies including pulmonary, renal and intestinal fibrosis, osteoarthr...
Bioinstructive microparticles for self-assembly of mesenchymal stem Cell-3D tum...
Ferreira, L. P.; Gaspar, V. M.; Mano, J. F.3D multicellular tumor spheroids (3D-MCTS) that closely mimic in vitro the complex lung tumor microenvironment (TME) are highly desirable for screening innovative anti-cancer therapeutics. Despite significant improvements in mimicking lung TME, few models have combined tumor-infiltrating mesenchymal stem cells from bone marrow (hBM-MSCs) with heterotypic 3D tumor spheroid models containing ECM mimetic component...
The biological performance of purified supercoiled p53 plasmid DNA in different...
Valente, J. F. A.; Sousa, A.; Gaspar, V. M.; Queiroz, João; Fani, SousaTumor suppressor p53 remains one of the most interesting therapeutic targets in cancer gene therapy due to itsconsistent mutation in numerous cancers. Thus, the reinstatement of the p53 expression and function can be seenas an effective alternative for cancer treatment, motivating research in thisfield. In this study,L-methioninematrix was used to purify the supercoiled topoisoform of a plasmid DNA encoding the...
3D tumor spheroids: an overview on the tools and techniques used for their anal...
Costa, Elisabete C.; Moreira, André; Diogo, Duarte Miguel de Melo; Gaspar, V. M.; Carvalho, Marco António Paulo de; Correia, I.J.In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some features of solid tumors, such as their spatial architecture, physiological responses, secretion of soluble mediators, gene expression patterns and drug resistance mechanisms. These unique characteristics highlight the potential of 3D cellular aggregates to be used as in vitro models for screening new anticancer therapeuti...
Improved Minicircle DNA Biosynthesis for Gene Therapy Applications
Gaspar, V. M.; Maia, C J; Queiroz, João; Pichon, Chantal; Correia, Ilídio; Sousa, FaniMinicircular DNA (mcDNA) biopharmaceuticals have recently risen as a valuable alternative for the development of a next generation of bioactive therapeutics because they are more efficient and safer than standard plasmid DNA (pDNA). To date, the relatively insufficient knowledge regarding mcDNA biosynthesis is currently hindering its manufacture in suitable amounts for clinical trial evaluations. Addressing thi...
Bioactive polymeric–ceramic hybrid 3D scaffold for application in bone tissue r...
Torres, Ana; Gaspar, V. M.; Serra, Inês Raquel Tavares; Carlos, Gabriela Soares Diogo; Fradique, Ricardo Gil; Silva, Abílio P.; Correia, I.J.The regeneration of large bone defects remains a challenging scenario from a therapeutic point of view. In fact, the currently available bone substitutes are often limited by poor tissue integration and severe host inflammatory responses, which eventually lead to surgical removal. In an attempt to address these issues, herein we evaluated the importance of alginate incorporation in the production of improved an...