99 documents found, page 1 of 10
Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 Akita
Carvalho, Filipe R.; Calado, Sofia M.; Silva, Gabriela A.; Diogo, Gabriela S.; Moreira-Silva, Joana; Reis, R. L.; Cancela, M. Leonor; Gavaia, Paulo J.Type 1 diabetes mellitus (T1DM) has been associated to several cartilage and bone alterations including growth retardation, increased fracture risk, and bone loss. To determine the effect of long term diabetes on bone we used adult and aging Ins2(Akita) mice that developed T1DM around 3-4 weeks after birth. Both Ins2(Akita) and wild-type (WT) mice were analyzed at 4, 6, and 12 months to assess bone parameters s...
Red algal extracts from Plocamium lyngbyanum and Ceramium secundatum stimulate ...
Carson, Matthew A.; Nelson, John; Cancela, M. Leonor; Laizé, Vincent; Gavaia, Paulo J.; Rae, Margaret; Heesch, Svenja; Verzin, Eugene; Maggs, ChristineThrough the current trend for bioprospecting, marine organisms-particularly algae-are becoming increasingly known for their osteogenic potential. Such organisms may provide novel treatment options for osteoporosis and other musculoskeletal conditions, helping to address their large healthcare burden and the limitations of current therapies. In this study, extracts from two red algae-Plocamium lyngbyanum and Cer...
Solea senegalensis sperm cryopreservation: new insights on sperm quality
F RIESCO, MARTA; Oliveira, Catarina; Soares, Florbela; Gavaia, Paulo J.; Dinis, Maria Teresa; Cabrita, ElsaCryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to iden...
Quantitative assessment of the regenerative and mineralogenic performances of t...
Cardeira, João; Gavaia, Paulo J.; Fernández, Ignacio; Cengiz, I. F.; Silva, Joana M.; Oliveira, J. M.; Reis, R. L.; Cancela, M. Leonor; Laizé, VincentThe ability of zebrafish to fully regenerate its caudal fin has been explored to better understand the mechanisms underlying de novo bone formation and to develop screening methods towards the discovery of compounds with therapeutic potential. Quantifying caudal fin regeneration largely depends on successfully measuring new tissue formation through methods that require optimization and standardization. Here, we...
Quantitative assessment of the regenerative and mineralogenic performances of t...
Cardeira Da Silva, João; Gavaia, Paulo J.; Fernandez, Ignacio; Cengiz, Ibrahim Fatih; Moreira-Silva, Joana; Oliveira, Joaquim Miguel; Reis, Rui L.The ability of zebrafish to fully regenerate its caudal fin has been explored to better understand the mechanisms underlying de novo bone formation and to develop screening methods towards the discovery of compounds with therapeutic potential. Quantifying caudal fin regeneration largely depends on successfully measuring new tissue formation through methods that require optimization and standardization. Here, we...
Glucose overload in yolk has little effect on the long-term modulation of carbo...
Rocha, Filipa; Dias, J.; Engrola, S.; Gavaia, Paulo J.; Geurden, Inge; Dinis, Maria Teresa; Panserat, S.The use of early nutritional stimuli to program metabolic pathways in fish is ill defined. Therefore, studies were undertaken with zebrafish to assess the effect of high glucose levels during the embryonic stage as a lifelong modulator of genes involved in carbohydrate metabolism. Genes related to carbohydrate metabolism were expressed at low levels at 0.2 and 1 day post-fertilization (dpf). However, from 4 dpf...
Factors enhancing fish sperm quality and emerging tools for sperm analysis
Cabrita, Elsa; Martínez-Páramo, S.; Gavaia, Paulo J.; Riesco, M. F.; Valcarce, D. G.; Sarasquete, C.; Herráez, M. P.; Robles, V.With this review we try to give a comprehensive overview of the current methods used in research to assess sperm quality. In addition, we identify some of the most important factors for enhancing sperm production and quality, including, broodstock nutrition, epigenetics and sperm management (cryopreservation). Sperm quality can be assessed by analyzing different parameters fromsimplemethods to very sophisticate...
Programming the metabolic gene expression in zebrafish juveniles (Danio rerio) ...
Rocha, F.; Dias, J.; Engrola, S.; Gavaia, Paulo J.; Geurden, Inge; Dinis, Maria Teresa; Panserat, S.Using zebrafish as a model organism, assess the effect of high glucose levels during early embryonic stages as a lifelong moderator of metabolic pathways.
Skeletal anomalies in reared European fish larvae and juveniles. Part 2: main t...
Boglione, C.; Gisbert, Enric; Gavaia, Paulo J.; Witten, Paul; Moren, M.; Fontagné, Stéphanie; Koumoundouros, GiorgosThe presence of skeletal anomalies in farmed teleost fish is currently a major problem in aquaculture, entailing economical, biological and ethical issues. The common occurrence of skeletal deformities in farmed fish and the absence of effective solutions for avoiding their onset or definitely culling out the affected individuals as early as possible from the productive cycle, highlight the need to improve our ...
Solea senegalensis vasa transcripts: molecular characterisation, tissue distrib...
Pacchiarini, T.; Cross, I.; Leite, Ricardo; Gavaia, Paulo J.; Ortiz-Delgado, J. B.; Pousão-Ferreira, P.; Rebordinos, L.; Sarasquete, C.; Cabrita, ElsaThe Vasa protein is an RNA helicase belonging the DEAD (Asp-Glu-Ala-Asp)-box family. The crucial role played by the vasa gene in the germ-cell lineage of both vertebrates and invertebrates has made this gene a useful molecular marker for germinal cells and a useful tool in surrogate broodstock production using primordial germ cell transplantation. With the aim of establishing a novel approach to improving Solea...