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Ionic liquid-based nanoemulsion as a promising strategy to enhance the bioacces...

Sousa, Vítor; Lüdtke, Fernanda Luisa; Hijo, Ariel A. C. Toledo; Loureiro, Luís; Dias, Oscar; Vicente, A. A.; Pinheiro, A. C.; Geada, Pedro

Dunaliella salina is a unicellular halophile green microalga that presents the capacity to accumulate high amounts of β-carotene, a bioactive compound widely recognized by its health benefits. To fulfil its biological functions, however, β-carotene needs to be bioaccessible, which is challenging as its bioaccessibility is typically low. A promising strategy to overcome β-carotene's low bioaccessibility is its e...


Comparison of different pretreatment processes envisaging the potential use of ...

Marques, Fabiana; Pereira, Francisco; Machado, Luís; Martins, Joana T.; Pereira, Ricardo N.; Costa, Monya; Genisheva, Zlatina; Pereira, Hugo

A significant fraction of the food produced worldwide is currently lost or wasted throughout the supply chain, squandering natural and economic resources. Food waste valorization will be an important necessity in the coming years. This work investigates the ability of food waste to serve as a viable nutritional substrate for the heterotrophic growth of Chlorella vulgaris. The impact of different pretreatments o...


Optimization of key growth parameters involved in biomass production of Pavlova...

Maciel, Filipe; Couto, D.; Geada, Pedro; Pereira, H.; Teixeira, J. A.; Domingues, M.; Silva, J.; Vicente, A. A.

Microalgae have been presented as an interesting source of bioactive compounds for human consumption in several industries, such as nutraceutical, pharmaceutical or cosmeceutical. Their rich composition in anti-inflammatory and antioxidant compounds (e.g., polyunsaturated fatty acids (PUFAs), proteins) have shown protective effects against several human diseases (e.g., cardiovascular, cancer). Despite their inc...


Microalgae biomass as an alternative source of biocompounds: New insights and f...

Sousa, Vítor; Pereira, Ricardo Nuno Correia; Vicente, A. A.; Dias, Oscar; Geada, Pedro

Microalgae have characteristics that make them unique and full of potential. Their capacity to generate interesting bioactive molecules can add value to various industrial applications. However, most of these valuable compounds are intracellular, which makes their extraction a major bottleneck. Conventional extraction methodologies have some drawbacks, such as low eco-friendly character, high costs and energy d...


Multivariable optimization process of heterotrophic growth of Chlorella vulgaris

Geada, Pedro; Francisco, Diogo; Pereira, Francisco; Filipe Maciel; Madureira, Leandro; Barros, Ana; Silva, Joana L.; Vicente, A. A.; Teixeira, J. A.

Microalgae have received increasing attention as one of the most promising feedstocks in the development of new healthier food products and different strategies have been attempted to improve their growth. However, the high production costs and low productivities, commonly associated with photoautotrophic growths, are still a big challenge. In this study, a two-step optimization strategy was carried out in orde...


Systems biology's role in leveraging microalgal biomass potential: Current stat...

Cunha, Emanuel; Sousa, Vítor; Geada, Pedro; Teixeira, J. A.; Vicente, A. A.; Dias, Oscar

Microalgae are an eco-friendly and alternative source of several compounds that can be applied in numerous biotechnological branches such as cosmetics, food, or pharmaceutical industries. However, the commercialization of products from microalgae has still some drawbacks, among which it is possible to highlight the low biomass and compounds' productivities at industrial level. The common strategies to improve t...


Effects of moderate electric fields on microalgae cell structure - novel perspe...

Machado, Luís Carlos Rodrigues; Geada, Pedro; Teixeira, J. A.; Pereira, Ricardo Nuno Correia

The use of microalgae in food industry has started to gain some interest due to their unique and promising characteristics. They are well known to present several compounds of interest such as proteins and essential amino acids with high nutritional impact for human and animal health. Usually, microalgae present a complex cellular structure, composes by strong polysaccharides, which affects their digestibility,...


Optimization of Pavlova gyrans biomass production and the fatty acid profile us...

Maciel, Filipe; Couto, Daniela; Geada, Pedro; Pereira, Hugo; Teixeira, José; Domingues, M. Rosário; Silva, Joana; Vicente, António

This work aimed to optimize and characterize the biomass production of Pavlova gyrans through a sequential multivariate approach. Among seventeen cultivation parameters, light intensity, NaNO3, and CuSO45H(2)O were identified as the most significative factors (p < 0.10) for biomass productivity. Together with NaH2PO4H2O, these variables were further studied in order to maximize the biomass production of P. gyra...


Multivariable approach on growth of microalgae

Geada, Pedro; Madureira, Leandro; Francisco, Diogo; Maciel, Filipe; Silva, Joana L.; Vicente, A. A.; Teixeira, J. A.

Despite the increasing interest in microalgal biomass and its high-value compounds, the number of products that successfully reach the market is still neglectable when compared to the high potential linked to these organisms. High production costs and low productivity both in terms of biomass and metabolites associated with microalgae growth are some of the causes hindering their widespread use and commercializ...


Cultivation systems of Coelastrella sp. isolated from lichens collected in the ...

Loureiro, Luís Carlos Sá; Geada, Pedro; Vasconcelos, Vitor; Vicente, A. A.

There is a growing interest towards finding new bioactive compounds from natural, safe and sustainable sources and extremophilic microalgae have emerged as one of the most promising options. Lichens are constituted by microalgae/cyanobacteria cells associated with a fungus that are subjected to especially extreme conditions, resulting in the production of a great variety of specific biologically active metaboli...


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