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Non-Mature miRNA-encoded micropeptide miPEP166c stimulates anthocyanin and proa...

Vale, Mariana; Badim, Hélder; Gerós, H.; Conde, Artur Jorge Silva

The phenylpropanoid and flavonoid pathways exhibit intricate regulation, not only influenced by environmental factors and a complex network of transcription factors but also by post-transcriptional regulation, such as silencing by microRNAs and miRNA-encoded micropeptides (miPEPs). VviMYBC2-L1 serves as a transcriptional repressor for flavonoids, playing a crucial role in coordinating the synthesis of anthocyan...


Impacto da implantação da democracia na evolução da ciência e formação avançada...

González-Méijome, José Manuel; Gerós, H.; Castro, Nuno Filipe

[Excerto] Neste ensaio pretendemos explorar a relação de retroalimentação mútua entre ciência e liberdade. De entre os múltiplos ângulos que poderiam ser explorados, em nossa opinião, quatro merecem uma especial atenção: a) relações de causalidade entre ciência e liberdade; b) liberdade como condição para o pensamento e criação científica livres; ou c) os direitos e responsabilidades da liberdade científica; e ...


Early activation of antioxidant responses in Ni-stressed tomato cultivars deter...

Spormann, Sofia; Soares, Cristiano; Martins, Viviana; Azenha, Manuel; Gerós, H.; Fidalgo, Fernanda

Throughout their life cycle, plants are subjected to a variety of environmental constraints, including abiotic stresses. The present study aimed at characterizing the responses of the two tomato cultivars Gold Nugget (GN) and Purple Calabash (PC) exposed to a combination of nickel (Ni) and drought. The following hypotheses were pursued: (i) the activation of responses to one stressor eases further adjustments t...


Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 displ...

Terra-Matos, Joana; Teixeira, Marta Oliveira; Pereira, Cátia Sofia Santos; Noronha, Henrique; Domingues, Lucília; Sieiro, Carmen; Gerós, H.

Yeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Understanding the mechanisms involved...


Constitutive expression of VviNAC17 transcription factor significantly induces ...

Badim, Hélder; Vale, Mariana; Coelho, Marco; Granell, Antonio; Gerós, H.; Conde, Artur Jorge Silva

VviNAC17 is a grapevine transcription factor activated by ABA. Because ABA has been proposed as the main signal modulating the secondary metabolism in grape berry skins, here we postulated VviNAC17 as a positive regulator of secondary metabolism in grape cells. To validate the hypothesis, VviNAC17 was constitutively and stably overexpressed in grape berry suspension-cultured cells of Gamay Fréaux cv. by Agrobac...


Impact of deficit irrigation on grapevine cv. ‘Touriga Nacional’ during three s...

Cabral, Inês L.; Teixeira, J. A.; Lanoue, Arnaud; Unlubayir, Marianne; Munsch, Thibaut; Valente, Joana; Alves, Fernando; Costa, Pedro Leal da

The introduction of irrigation in vineyards of the Mediterranean basin is a matter of debate, in particular in those of the Douro Demarcated Region (DDR), due to the limited number of available studies. Here, we aimed to perform a robust analysis in three consecutive vintages (2018, 2019, and 2020) on the impact of deficit irrigation on the yield, berry quality traits, and metabolome of cv. ‘Touriga Nacional’. ...


VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine wood...

Noronha, Henrique; Silva, Angélica; Silva, Tiago; Frusciante, Sarah; Diretto, Gianfranco; Gerós, H.

The accumulation of raffinose family oligosaccharides (RFOs) is a hallmark of plant response to different abiotic stresses, including cold. The synthesis of galactinol, by galactinol synthases (GolS), and raffinose, by raffinose synthases (RafS), are fundamental for stress-induced accumulation of RFOs, but the role of these enzymes in the cold response of grapevine (Vitis vinifera L.) woody tissues is still unc...


Grapevine aquaporins: Diversity, cellular functions, and ecophysiological persp...

Sabir, Farzana; Zarrouk, Olfa; Noronha, Henrique Luís Silva; Loureiro-Dias, Maria C.; Soveral, Graça; Gerós, H.; Prista, Catarina

High-scored premium wines are typically produced under moderate drought stress, suggesting that the water status of grapevine is crucial for wine quality. Aquaporins greatly influence the plant water status by facilitating water diffusion across the plasma membrane in a tightly regulated manner. They adjust the hydraulic conductance of the plasma membrane rapidly and reversibly, which is essential in specific p...


Exogenous calcium delays grape berry maturation in the white cv. loureiro while...

Martins, Viviana; Unlubayir, Marianne; Teixeira, António; Lanoue, Arnaud; Gerós, H.

Vineyard calcium (Ca) sprays have been increasingly used by grape growers to improve fruit firmness and thus maintain quality, particularly in periods of heavy rains and hail. The observation that Ca visibly modified berry size, texture, and color in the most prominent white cultivar of the DOC region Vinhos Verdes, cultivar (cv.) Loureiro, led us to hypothesize that Ca induced metabolic rearrangements that res...


The restructuring of grape berry waxes by calcium changes the surface microbiota

Martins, Viviana; Szakiel, Anna; Paczkowski, Cezary; Teixeira, António; Gerós, H.

The observation that exogenous Ca shifted the polyphenolic profile of grape berries and conferred a glossy appearance to mature fruits led us to hypothesize that the composition of grape berry waxes and thus surface microbiota are modified. In two cultivars sharing the same microclimate, the triterpenoid and steroid profile of berry cuticle was characterized by a targeted metabolomic approach, and surface micro...


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