The expression of milk proteins in vitro is essential to exploit the mammary gland cells as a biological model. Enzymatic tissue disaggregation has been widely used to establish mammary cell culture, but its effect in long-term ovine mammary cell culture is not completely elucidated. This study aimed at comparing mechanical/enzymatic and mechanical dissociation methods to establish ovine mammary cell culture. W...
Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:23:48Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:30:16Z : No. of bitstreams: 1 2-s2.0-78149398352.pdf: 1261937 bytes, checksum: 38b7872db63fef0be50c3e74be6371be (MD5); Made available in DSpace on 2014-05-27T11:23:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-12-01; During the critical period of the ...
During the critical period of the maternal recognition, which occurs between days 15 and 19 of pregnancy, the conceptus must competently synthesize molecules capable of blocking the synthesis of prostaglandin F2α (PGF2α) and luteolysis. In cattle, the major macromolecule involved in suck blockage is the protein interferon-tau (IFN-τ). During the critical period, failures in the recognition of pregnancy determin...