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Efficient extraction of the RuBisCO enzyme from spinach leaves using aqueous so...
Valente, Ana I.; Ferreira, Ana M.; Almeida, Mafalda R.; Mohamadou, Aminou; Freire, Mara G.; Tavares, Ana P. M.Ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) is the most abundant protein on the planet, being present in plants, algae and various species of bacteria, with application in the pharmaceutical, chemical, cosmetic and food industries. However, current extraction methods of RuBisCO do not allow high yields of extraction. Therefore, the development of an efficient and selective RuBisCOs’ extraction meth...
Integrated approach to extract and purify proteins from honey by ionic liquid-b...
Pereira, Matheus M.; Pedro, Sónia N.; Quental, Maria V.; Mohamadou, Aminou; Coutinho, João A. P.; Freire, Mara G.The purification of value-added compounds by three phase partitioning (TPP) is a promising alternative to conventional processes since the target compound can be easily recovered from the liquid-liquid interphase. Although this technique has been successfully applied to the recovery of proteins, the minimization of the use of salts and solvents must be pursued to improve the overall process sustainability. Acco...
Sustainable strategies based on glycine–betaine analogue ionic liquids for the ...
Capela, Emanuel V.; Santiago, Alexandre E.; Rufino, Ana F. C. S.; Tavares, Ana P. M.; Pereira, Matheus M.; Mohamadou, Aminou; Aires-Barros, M. RaquelMonoclonal antibodies (mAbs) are of crucial interest for therapeutic purposes, particularly in vaccination and immunization, and in the treatment of life-threatening diseases. However, their downstream processing from the complex cell culture media in which they are produced still requires multiple steps, making mAbs extremely high-cost products. Therefore, the development of cost-effective, sustainable and bio...
Glycine-betaine-derived ionic liquids: Synthesis, characterization and ecotoxic...
Parajó, Juan J.; Macário, Inês P. E.; De Gaetano, Yannick; Dupont, Laurent; Salgado, Josefa; Pereira, Joana Luísa; Gonçalves, Fernando J. M.Ionic Liquids (ILs) are generically regarded as environmentally "harmless" and thus, assumed as "non-toxic". However, due to the endless design possibilities, their ecotoxicological profile is still poorly known. An accurate knowledge on the toxicity of a substance is required, under the scope of environmental regulation worldwide, before their application and commercialization. Knowledge on the relationship be...
Synthesis and characterization of analogues of glycine-betaine ionic liquids an...
Pereira, Matheus M.; Pedro, Sónia N.; Gomes, Joana; Sintra, Tânia E.; Ventura, Sónia P. M.; Coutinho, João A. P.; Freire, Mara G.; Mohamadou, AminouA series of novel analogues of glycine-betaine ionic liquids (AGB-ILs), viz. 1-(4-ethoxy-4-oxobutyl)-1-methylpyrrolidin-1-ium, N,N,N-tri(n-butyl)(4-ethoxy-4-oxobutyl)-1-phosphonium and N,N,N-trialkyl(4-ethoxy-4-oxobutyl)-1-aminium cations with ethyl, n-propyl and n-butyl alkyl chains, combined with the bromide anion, have been synthesized and characterized. Their synthesis and characterization by spectroscopic ...