3 documents found, page 1 of 1
Lacrimal gland primary acinar cell culture: the role of insulin
Malki,Leonardo Tannus; Dias,Ana Carolina; Jorge,Angelica Gobbi; Módulo,Carolina Maria; Rocha,Eduardo MelaniABSTRACT Purpose: The goal of the present study was to establish a protocol for primary culture of lacrimal gland acinar cells (LGACs) and to assess the effect of adding insulin to the culture media. Methods: LGACs were isolated and cultured from lacrimal glands of Wistar male rats. The study outcomes included cell number, viability, and peroxidase release over time and in response to three concentrations of in...
Insulin replacement restores the vesicular secretory apparatus in the diabetic ...
Dias,Ana Carolina; Batista,Thiago Martins; Roma,Letícia Prates; Módulo,Carolina Maria; Malki,Leonardo Tannus; Dias,Lara Cristina; Alves,MônicaABSTRACT Purpose: In the lacrimal gland (LG) acinar cells, signaling regulates the release of secretory vesicles through specific Rab and SNARE exocytotic proteins. In diabetes mellitus (DM), the LGs are dysfunctional. The aim of this work was to determine if secretory apparatus changes were associated with any effects on the secretory vesicles (SV) in diabetic rats as well as the expression levels of constitue...
Tear film and ocular surface alterations in chronic users of antiglaucoma medic...
Baffa,Lina do Prado; Ricardo,Jose Reinaldo da Silva; Dias,Ana Carolina; Módulo,Carolina Maria; Braz,Alexandre Martins; Paula,Jayter Silva dePURPOSE: Tear film can be altered by chronic medications that may disrupt the equilibrium responsible for the functioning of the lacrimal gland and ocular surface. The purpose of this study was to determine if antiglaucomatous chronic treatment induced alterations in the tear film and ocular surface. METHODS: After informed consent, 21 patients using antiglaucomatous eye drops for more than 8 months and 20 age-...