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iOD907, the first genome-scale metabolic model for the milk yeast Kluyveromyces...

Dias, Oscar; Pereira, R. M.; Gombert, Andreas Karoly; Ferreira, Eugénio C.; Rocha, I.

We describe here the first genome-scale metabolic model of Kluyveromyces lactis, iOD907. It is partially compartmentalized (4 compartments), composed of 1867 reactions and 1476 metabolites. The iOD907 model performed well when assessing the positive growth of K. lactis to Biolog experiments and to an online catalogue of strains that provides information on carbon sources in which K. lactis is able to grow. Chem...


Towards a genome-scale metabolic model for the Kluyveromyces lactis yeast

Dias, Oscar; Gombert, Andreas Karoly; Ferreira, Eugénio C.; Rocha, I.

The interest in Kluyveromyces lactis (K. lactis) has begun in academia due to its ability to metabolize the betaglycoside (1). Since then, this yeast has been considered a model organism for studies in genetics and physiology (2). This yeast had its genome sequenced back in 2004 (3) and recently we have published a full metabolic re-annotation of its genome (4). This re-annotation can be used, among other appli...


iOD962 - the first genome-scale metabolic model of Kluyveromyces lactis

Dias, Oscar; Gombert, Andreas Karoly; Ferreira, Eugénio C.; Rocha, I.

The genome-scale metabolic model of Kluyveromyces lactis was reconstructed from its genome annotation. The result was the partially compartmentalized (5 compartments) iOD962 metabolic model composed of 2038 reactions and 1561 metabolites. Previous chemostate experiments were used to adjust the maintenance ATP parameter, and the model proved valuable when predicting the biomass, oxygen and carbon dioxide yields....


Genome-wide metabolic (re-) annotation of Kluyveromyces lactis

Dias, Oscar; Gombert, Andreas Karoly; Ferreira, Eugénio C.; Rocha, I.

Even before having its genome sequence published in 2004, Kluyveromyces lactis had long been considered a model organism for studies in genetics and physiology. Research on Kluyveromyces lactis is quite advanced and this yeast species is one of the few with which it is possible to perform formal genetic analysis. Nevertheless, until now, no complete metabolic functional annotation has been performed to the prot...




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