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Direct Restorations for Anterior Esthetic Rehabilitation and Smile Symmetry Rec...

Amaro, Inês; Saraiva, José; Gomes, Ana Catarina; Araújo, Ana; Marto, Carlos Miguel; Coelho, Ana; Ferreira, Manuel Marques; Paula, Anabela

Esthetics is an increasing concern for patients, particularly in anterior teeth. The current approaches to manage the esthetic demands include options such as prosthodontic rehabilitation, orthodontic treatment, and direct restorations. Direct restorative procedures for anterior teeth have become a reliable alternative, since current composite materials can mimic teeth’s esthetics and function perfectly. This a...


Fecal microbiota transplantation in the intestinal decolonization of carbapenam...

Silva, João Carlos; Ponte, Ana; Mota, Margarida; Pinho, Rolando; Vieira, Nuno; Oliveira, Rosa; Mota-Carvalho, Nelson; Gomes, Ana Catarina; Afecto, Edgar

Background and aims: fecal microbiota transplantation (FMT) is effective for recurrent Clostridium difficile infection (CDI). Intestinal decolonization of carbapenamase-producing enterobacteriaceae (CPE) can prevent transmission and infection by these agents. The aim of this study was to assess CPE decolonization after FMT. Methods: this was a case-series study that consecutively included all CPE-carriers that ...


Whole-genome DNA sequencing: the key to detecting a sarcomeric mutation in a ‘f...

Gomes, Ana Catarina; Barbosa, Pedro Santos; Coutinho, Ana; Cruz, Inês; Carmo-Fonseca, Maria; Lopes, Luís Rocha

The authors report the clinical and genetic investigation of a family with hypertrophic cardiomyopathy (HCM). The individuals described are three affected first-degree relatives (father, daughter and son), one affected niece and unaffected nephew and niece. Those affected all share a very similar phenotype consisting of asymmetric HCM, with hypertrophy particularly affecting the septum and the anterior wall, an...


Risk factors for in-hospital mortality in infective endocarditis

Marques, Ana; Cruz, Inês; Caldeira, Daniel; Alegria, Sofia; Gomes, Ana Catarina; Luísa Broa, Ana; João, Isabel; Pereira, Helder

Background: Infective endocarditis (IE) is associated with severe complications and high mortality. The assessment of mortality rates and predictors for fatal events is important to identify modifiable factors related to the pattern of treatment, in order to improve outcomes. Objectives: We sought to evaluate clinical outcomes of patients with IE and to determine predictors of in-hospital mortality. Methods: Re...


Understand the Potential Role of Aureobasidium pullulans, a Resident Microorgan...

Pinto, Cátia; Custódio, Valéria; Nunes, Mariana; Songy, Aurélie; Rabenoelina, Fanja; Courteaux, Barbara; Clément, Christophe; Gomes, Ana Catarina

Grapevine trunk diseases (GTDs) are one of the major concern amongst grapevine diseases, responsible for the decline of vineyards and for several economical losses. Since grapevine is naturally colonized by resident microorganisms such as Aureobasidium pullulans, the present challenge is to understand their biocontrol potential and how such microorganisms can be successfully integrated in the control of GTDs. I...


Is the time in therapeutic range using the ratio of tests equivalent to the Ros...

Caldeira, Daniel; Cruz, Inês; Morgado, Gonçalo; Stuart, Bruno; Gomes, Ana Catarina; Martins, Cristina; João, Isabel; Pereira, Helder

The percentage of time in therapeutic range (TTR) is a measure of anticoagulation quality with vitamin K antagonists (VKAs). The method most commonly used in clinical trials is the Rosendaal TTR. However, the application of this method in daily practice for clinical decision lacks appropriate instruments. We aimed to evaluate the percentage of tests within the target international normalized ratio (INR) (tests ...


Genetic diversity and population structure of Saccharomyces cerevisiae strains ...

Schuller, Dorit Elisabeth; Cardoso, Filipa; Sousa, Susana; Gomes, Paula; Gomes, Ana Catarina; Santos, Manuel A. S.; Casal, Margarida

We herein evaluate intraspecific genetic diversity of fermentative vineyard-associated S. cerevisiae strains and evaluate relationships between grape varieties and geographical location on populational structures. From the musts obtained from 288 grape samples, collected from two wine regions (16 vineyards, nine grape varieties), 94 spontaneous fermentations were concluded and 2820 yeast isolates were obtained ...


Genotyping of Saccharomyces cerevisiae strains by interdelta sequence typing us...

Duarte, Ricardo Franco; Gomes, Ana Catarina; Santos, Manuel A. S.; Sousa, Bruno; Schuller, Dorit Elisabeth; Mendes, Inês

Amplification of genomic sequences flanked by delta elements of retrotransposons TY1 and TY2 is a reliable method for characterization of Saccharomyces cerevisiae strains. The aim of this study is to evaluate the usefulness of microfluidic electrophoresis (Caliper LabChip®) to assess the factors that affect interlaboratory reproducibility of interdelta sequence typing for S. cerevisiae strain delimitation. We c...


Yeast diversity related with Touriga Nacional Grape variety

Duarte, Filomena; Teixeira, A.; Ramos, P.; Gomes, Ana Catarina; Schuller, Dorit Elisabeth; Alemão, F.; Costa, Ana Catarina Moreira da

The diversity of yeast species related with the grape variety Touriga Nacional was evaluated at four distinct vineyards of three Portuguese wine regions. Samples were collected 48 h after crushing, and when the loss of CO2 reached 70 g/L. Restriction profiles analysis of 26S rDNA region with the endonucleases HaeIII, CfoI, HinfI, ApaI and MseI was used to characterize the five hundred and nine isolates obtained...


Genotyping of Saccharomyces cerevisiae strains by interdelta sequence typing us...

Franco-Duarte, Ricardo; Mendes, Inês; Gomes, Ana Catarina; Santos, Manuel A. S.; de Sousa, Bruno; Schuller, Dorit

Amplification of genomic sequences flanked by delta elements of retrotransposons TY1 and TY2 is a reliable method for characterization of Saccharomyces cerevisiae strains. The aim of this study is to evaluate the usefulness of microfluidic electrophoresis (Caliper LabChips) to assess the factors that affect interlaboratory reproducibility of interdelta sequence typing for S. cerevisiae strain delimitation. We carr...


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