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Settled dust assessment in clinical environment: useful for the evaluation of a...

Viegas, Carla; Almeida, Beatriz; Monteiro, Ana; Paciência, Inês; Rufo, João Cavaleiro; Carolino, Elisabete; Gomes, Anita Quintal; Twarużek, Magdalena

The collection and analysis of settled dust samples from indoor environments has become one of several environmental sampling methods used to assess bioburden indoors. The aim of the study was to characterize the bioburden in vacuumed settled dust from 10 Primary Health Care Centers by culture-based and molecular methods. Results for bacterial load ranged from 1 to 12 CFU.g−1 of dust and Gram-negative bacteria ...


Bioburden in sleeping environments from Portuguese dwellings

Viegas, Carla; Dias, Marta; Monteiro, Ana; Faria, Tiago; Lage, Joana; Carolino, Elisabete; Caetano, Liliana Aranha; Gomes, Anita Quintal

A wider characterization of indoor air quality during sleep is still lacking in the literature. This study intends to assess bioburden before and after sleeping periods in Portuguese dwellings through active methods (air sampling) coupled with passive methods, such as electrostatic dust cloths (EDC); and investigate associations between before and after sleeping and bioburden. In addition, and driven by the lac...


Aspergillus spp. burden on filtering respiratory protective devices: is there a...

Viegas, Carla; Dias, Marta; Almeida, Beatriz; Carolino, Elisabete; Gomes, Anita Quintal; Viegas, Susana

There is scientific evidence that associates occupational exposure to fungi in the waste industry with health outcomes, being Aspergillus genera reported as the most prevalent. During Filtering Respiratory Protective Devices (FFR) use, humidity and temperature conditions provide favorable conditions for Aspergillus growth. The aim of this study was to characterize the Aspergillus burden retained by the FFR inte...


Are workers from waste sorting industry really protected by wearing filtering r...

Viegas, Carla; Dias, Marta; Almeida, Beatriz; Aranha Caetano, Liliana; Carolino, Elisabete; Gomes, Anita Quintal; Twarużek, Magdalena; Kosicki, Robert

One of the solutions for decreasing the workers' exposure to bioburden is the use of Filtering Respiratory Protective Devices (FRPD). As such it is important to determine whether these devices are fulfilling their protective role. This is the basis of the current study, aimed at characterizing bioburden retained by 120 FRPD (both in interior layers and in exhalation valves) through culture based-methods and mol...


Exposure assessment in one central hospital: a multi-approach protocol to achie...

Viegas, Carla; Almeida, Beatriz; Monteiro, Ana; Paciência, Inês; Rufo, João; Aguiar, Lívia; Lage, Bruna; Diogo Gonçalves, Lídia Maria

The bioburden in a Hospital building originates not only from patients, visitors and staff, but is also disseminated by several indoor hospital characteristics and outdoor environmental sources. This study intends to assess the exposure to bioburden in one central hospital with a multi-approach protocol using active and passive sampling methods. The microbial contamination was also characterized through molecul...


Uncovering the role of microRNAs in the production of pro-inflammatory cytokies...

Inácio, Daniel; Amado, Tiago; Sobral, Daniel; Enguita, Francisco; Cunha, Carolina; Silva-Santos, Bruno; Gomes, Anita Quintal


Aspergillus spp. prevalence in Primary Health Care Centres: assessment by a nov...

Viegas, Carla; Almeida, Beatriz; Gomes, Anita Quintal; Carolino, Elisabete; Aranha Caetano, Liliana

Exposure to Aspergillus conidia may cause adverse effects on human health; however, no specific recommendations for routine assessments of Aspergillus in the clinical environment have been suggested so far. This study intended to determine the prevalence of Aspergillus in the clinical environment, focusing on ten Primary Health Care Centres (PHCC) through a novel multi-approach sampling protocol. Air and passiv...


Electrostatic dust collector: a passive screening method to assess occupational...

Viegas, Carla; Santos, Patrícia; Almeida, Beatriz; Monteiro, Ana; Carolino, Elisabete; Gomes, Anita Quintal; Viegas, Susana

Passive methods, such as electrostatic dust collectors (EDCs), allow determination of contamination levels for a long period of time, while air samples can only reproduce the load for a shorter period of time. We intended to analyze the suitability of EDC for identifying critical workstations of occupational exposure to particulate matter and for characterizing the bioburden present in ten primary health care c...


Uncovering the role of microRNAs in the control of effector gd T cell different...

Inácio, Daniel; Amado, Tiago; Sobral, Daniel; Enguita, Francisco; Silva-Santos, Bruno; Gomes, Anita Quintal


γδ-T cells promote IFN-γ–dependent Plasmodium pathogenesis upon liver-stage inf...

Ribot, Julie C.; Neres, Rita; Zuzarte-Luís, Vanessa; Gomes, Anita Quintal; Mancio-Silva, Liliana; Mensurado, Sofia; Pinto-Neves, Daniel

Cerebral malaria (CM) is a major cause of death due to Plasmodium infection. Both parasite and host factors contribute to the onset of CM, but the precise cellular and molecular mechanisms that contribute to its pathogenesis remain poorly characterized. Unlike conventional αβ-T cells, previous studies on murine γδ-T cells failed to identify a nonredundant role for this T cell subset in experimental cerebral mal...


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