12 documents found, page 1 of 2
Forensic interview techniques in child sexual abuse cases: a scoping review
Fernandes, Delfina Manuela Silva; Gomes, João P.; Albuquerque, Pedro Barbas; Matos, MarleneChild sexual abuse (CSA) is widely recognized as a global public health problem with negative consequences for victims, their families, and society. The child’s testimony is essential to the case outcome, given the frequent absence of physical or biological evidence of the abusive acts. Thus, the child forensic interview plays a decisive role in criminal investigation. The present scoping review aims to identif...
Distribution and Clinical Significance of HPV16 Variants in Head and Neck Squam...
Cochicho, Daniela; Nunes, Alexandra; Sobral, Daniel; Gomes, João P.; Esteves, Susana; Mendonça, Joana; Vieira, Luis; Martins, Luís; Cunha, MarioIntroduction: Genomic variants of the human papillomavirus type 16 (HPV16) are thought to play differential roles in the susceptibility to head and neck squamous cell carcinomas (HNSCC) and its biological behaviour. This study aimed to establish the prevalence of HPV16 variants in an HNSCC cohort and associate them with clinical pathological characteristics and patient survival. Methods: We retrieved samples an...
Development of a self-diagnostic system integrated into a cyber-physical system
Oliveira, Domingos F.; Gomes, João P.; Pereira, Ricardo B.; Brito, Miguel A.; Machado, Ricardo J.CONTROLAR provides Bosch with an intelligent functional testing machine used to test the correct functioning of the car radios produced. During this process, the radios are submitted to several tests, raising the problem of how the machine detects errors in several radios consecutively, making it impossible to know if the device has a problem since it has no module to see if it works correctly. This article ari...
Tracking SARS-CoV-2 lineage B.1.1.7 dissemination: insights from nationwide spi...
Borges, Vítor; Sousa, Carlos; Menezes, Luís; Gonçalves, António Maia; Picão, Miguel; Almeida, José Pedro; Vieita, Margarida; Santos, RafaelWe show that the SARS-CoV-2 B.1.1.7 lineage is highly disseminated in Portugal, with the odds of B.1.1.7 proportion increasing at an estimated 89% (95% confidence interval: 83-95%) per week until week 3 2021. RT-PCR spike gene target late detection (SGTL) can constitute a useful surrogate to track B.1.1.7 spread, besides the spike gene target failure (SGTF) proxy. SGTL/SGTF samples were associated with statisti...
Potential recurrence of COVID-19 in a healthcare professional: SARS-CoV-2 genom...
Correia, Ana M.; Borges, Vítor; Isidro, Joana; Lima, Ana R.; Fernandes, Alberto; Godinho, Maria Leonor; Duarte, Sílvia; Ferrão, José; Vieira, LuísRe-positivity of SARS-CoV-2 tests is widely reported, raising discussion about guidance for patient discharge and ending isolation. The unsuccessful recovery of replication-competent virus and/or absence of secondary cases has suggested that re-positive patients are not contagious. This study reports SARS-CoV-2 re-positivity in a healthcare professional 16 days after three negative tests, with viral genome sequ...
MRSA CC398 recovered from wild boar harboring new SCCmec type IV J3 variant
Sousa, Margarida; Silva, Nuno; Borges, Vítor; Gomes, João P.; Vieira, Luís; Caniça, Manuela; Torres, Carmen; Igrejas, Gilberto; Poeta, PatríciaA methicillin-resistant Staphylococcus aureus CC398 was recovered from a wild female boar (Sus scrofa) in the north of Portugal, in 2013 (Sousa et al. 2017). Whole genome sequencing (WGS) revealed this strain carries a new variant of a mecA-containing staphylococcal chromosomal gene cassette (SCCmec) type IV with an uncommon J3 region. WGS studies can facilitate surveillance and provide more detailed characteri...
Multiomics Assessment of Gene Expression in a Clinical Strain of CTX-M-15-Produ...
Pinto, Luís; Torres, Carmen; Gil, Concha; Nunes-Miranda, Júlio D.; Santos, Hugo M.; Borges, Vítor; Gomes, João P.; Silva, Catarina; Vieira, LuísExtended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strain C999 was isolated of a Spanish patient with urinary tract infection. Previous genotyping indicated that this strain presented a multidrug-resistance phenotype and carried beta-lactamase genes encoding CTX-M-15, TEM-1, and OXA-1 enzymes. The whole-cell proteome, and the membrane, cytoplasmic, periplasmic and extracellular sub-proteomes of ...
Genomic structure and insertion sites of Helicobacter pylori prophages from var...
Vale, Filipa F.; Nunes, Alexandra; Oleastro, Mónica; Gomes, João P.; Sampaio, Daniel A.; Rocha, Raquel; Vítor, Jorge M. B.; Engstrand, Lars; Pascoe, BenHelicobacter pylori genetic diversity is known to be influenced by mobile genomic elements. Here we focused on prophages, the least characterized mobile elements of H. pylori. We present the full genomic sequences, insertion sites and phylogenetic analysis of 28 prophages found in H. pylori isolates from patients of distinct disease types, ranging from gastritis to gastric cancer, and geographic origins, coveri...
In Vitro Activity of Quaternary Ammonium Surfactants against Streptococcal, Chl...
Inácio, Ângela S.; Nunes, Alexandra; Milho, Catarina; Mota, Luís Jaime; Borrego, Maria J.; Gomes, João P.; Vaz, Winchil L.C.; Vieira, Otília V.Quaternary ammonium compounds (QAC) are widely used, cheap, and chemically stable disinfectants and topical antiseptics with wide-spectrum antimicrobial activities. Within this group of compounds, we recently showed that there are significant differences between the pharmacodynamics of n-alkyl quaternary ammonium surfactants (QAS) with a short (C12) alkyl chain when in vitro toxicities toward bacterial and mamm...
Legionella pneumophila strain associated with the first evidence of person-to-p...
Borges, Vítor; Nunes, Alexandra; Sampaio, Daniel A.; Vieira, Luís; Machado, Jorge; Simões, Maria J.; Gonçalves, Paulo; Gomes, João P.A first strong evidence of person-to-person transmission of Legionnaires' Disease (LD) was recently reported. Here, we characterize the genetic backbone of this case-related Legionella pneumophila strain ("PtVFX/2014"), which also caused a large outbreak of LD. PtVFX/2014 is phylogenetically divergent from the most worldwide studied outbreak-associated L. pneumophila subspecies pneumophila serogroup 1 strains. ...