3 documents found, page 1 of 1
Effect of multiple freeze-thaw cycles on the stability of positive anti-trepone...
Castejon,Marcia Jorge; Yamashiro,Rosemeire; Oliveira,Camila C.; Oliveira,Elaine L.; Silveira,Edilene P. R.; Oliveira,Carmem Aparecida F.ABSTRACT Introduction: Biological samples have long been used in multiple laboratory investigations, and this procedure has been an issue of concern, as the samples are submitted to repeated freeze-thaw cycles, which may affect the results of a particular immunodiagnostic assay, due to the occurrence of physical damage to the antibody of interest. Objective: This study aimed at investigating the impact of succe...
Study on the stability of HIV and syphilis reference materials under varied tem...
Castejon,Márcia J.; Yamashiro,Rosemeire; Oliveira,Camila C.; Oliveira,Elaine L.; Silveira,Edilene P. R.; Oliveira,Carmem Aparecida F.ABSTRACT Introduction: The objective of this study was to evaluate the short-term stability of the serum samples used as internal quality control (IQC) of human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (Aids) and syphilis immunodiagnostic assays. These samples were produced at the Center of Immunology-Instituto Adolfo Lutz (IAL), and they were distributed to laboratories participating in ...
Comparative study of five commercial probes for the detection of Epstein-Barr v...
Nonogaki,Suely; Shirata,Neuza K.; Kimura,Lidia M.; Guerra,Juliana M.; Medeiros,Raphael S. S.; Paes,Roberto Antonio P.; Kanamura,Cristina T.ABSTRACT Introduction: Epstein-Barr virus (EBV) may serve as a target in therapeutic treatments, thus reliable diagnostic results are necessary. Objective: The aim of this study was to evaluate the accuracy of EBV detection by in situ hybridization (ISH) using five commercial probes in formalin-fixed and paraffin-embedded samples of nodular sclerosis Hodgkin's lymphoma (HL), and to compare the results with immu...