13 documents found, page 1 of 2
Male Swiss mice (Mus musculus) as a most suitable experimental model for the st...
Romera, Liara Izabela Lopes; Bezagio, Renata Coltro; Ferreira, Willian Costa; Almeida, Caroline Rodrigues de; Gomes, Mônica LúciaIn this study, we proposed to verify the most suitable murine experimental model for studying human giardiasis. In total 150 animals were used. Fifty mice (Mus musculus) from each lineage (Swiss, Balb/c and C57BL/6), 25 females and 25 males, were divided into 5 groups with 5 animals each, according to the lineage/sex. Three groups were infected with 104 cysts of Giardia duodenalis of assemblage BIV and 2 negati...
Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms ...
Monteiro, Wuelton Marcelo; Teston, Ana Paula Margioto; Gruendling, Ana Paula; Reis, Daniele dos; Gomes, Mônica Lúcia; Araújo, Silvana Marques deBackground: In the Brazilian Amazon, clinical and epidemiological frameworks of Chagas disease are very dissimilar in relation to the endemic classical areas of transmission, possibly due to genetic and biological characteristics of the circulating Trypanosoma cruzi stocks. Twenty six T. cruzi stocks from Western Amazon Region attributed to the TcI and TcIV DTUs were comparatively studied in Swiss mice to test ...
Unequivocal identification of subpopulations in putative multiclonal Trypanosom...
Valadares, Helder Magno Silva; Pimenta, Juliana Ramos; Segatto, Marcela; Veloso, Vanja Maria; Gomes, Mônica Lúcia; Chiari, Egler; Gollob, Kenneth JohnTrypanosoma cruzi, the etiological agent of Chagas disease, is a polymorphic species. Evidence suggests that the majority of the T. cruzi populations isolated from afflicted humans, reservoir animals, or vectors are multiclonal. However, the extent and the complexity of multiclonality remain to be established, since aneuploidy cannot be excluded and current conventional cloning methods cannot identify all the r...
Usefulness of the polymerase chain reaction for monitoring cure of mice infecte...
Miyamoto, Cláudia Tiemi; Gomes, Mônica Lúcia; Marangon, Aline Vansan; Araújo, Silvana Marques de; Bahia, Maria Terezinha; Martins Filho, Olindo AssisThe capacity of the polymerase chain reaction (PCR) to detect the DNA of Trypanosoma cruzi was evaluated in 90 blood samples from BALB/c mice infected with T. cruzi cloned stocks of genotypes 19 and 20 (T. cruzi I) and 39 and 32 (T. cruzi II), and treated with benznidazole. The results from the fresh blood examination, hemoculture, and ELISA allowed to group the treated animals into: cured (TC), dissociated (DI...
Highly diluted medication reduces parasitemia and improves experimental infecti...
Aleixo, Denise Lessa; Ferraz, Fabiana Nabarro; Ferreira, Érika Cristina; Lana, Marta de; Gomes, Mônica Lúcia; Abreu Filho, Benício Alvez deBackground: There is no published information about the use of different protocols to administer a highly diluted medication. Evaluate the effect of different protocols for treatment with biotherapic T. cruzi 17 dH (BIOTTc17dH) on clinical/parasitological evolution of mice infected with T. cruzi-Y strain. Methods: A blind, randomized controlled trial was performed twice, using 60 28-day-old male Swiss mice infe...
Trypanosoma cruzi : sensitivity of the polymerase chain reaction for detecting ...
Miyamoto, Cláudia Tiemi; Gomes, Mônica Lúcia; Marangon, Aline Vansan; Araújo, Silvana Marques de; Bahia, Maria Terezinha; Lana, Marta deThe polymerase chain reaction showed high sensitivity for detecting Trypanosoma cruzi in the blood of mice, independent of clonal genotype (19, 20—T. cruzi I; 32, 39—T. cruzi II) or phase of the infection (acute or chronic).
Induction of phagocytic activity and nitric-oxide production in natural populat...
Zalloum, Leila; Lala, Eliane Raquel Peres; Moreira, Neide Martins; Silveira, Thaís Gomes Verzignassi; Dalálio, Márcia Machado de OliveiraTwelve strains of Trypanosoma cruzi isolated from wild reservoirs, triatomines, and chronic chagasic patients in the state of Paraná, southern Brazil, and classified as T. cruzi I and II, were used to test the correlation between genetic and biological diversity. The Phagocytic Index (PI) and nitric-oxide (NO) production in vitro were used as biological parameters. The PI of the T. cruzi I and II strains did no...
Polymerase chain reaction and blood culture in blood donors screened by ELISA t...
Kinoshita-Yanaga, Andréa Tieko; Bértoli, Marta; Martins, Vanessa Aparecida; Mizutani, Angélica Sayuri; Toledo, Max Jean de OrnelasThe objective of this study was to evaluate, through blood culture and PCR, the results of the ELISA for Chagas' disease in the screening of blood donors in the public blood-supply network of the state of Paraná, Brazil, and to map the epidemiological profile of the donors with respect to their risk of infection by Trypanosoma cruzi. The negative and positive results of the ELISA were confirmed by blood culture...
Enteroparasitosis and their ethnographic relationship to food handlers in a tou...
Takizawa, Maria das Graças Marciano Hirata; Falavigna, Dina Lúcia Morais; Gomes, Mônica LúciaThis study reports on the occurrence of enteroparasites based on data from an ethnographic study of food handlers in the city of Cascavel, Paraná, Brazil. Fecal material from 343 food handlers of both sexes, between 14 and 75 years of age, was analyzed using Lutz, modified Ritchie and Ziehl-Neelsen techniques. Ethnographic relationships were investigated by means of specific questionnaires. Positive fecal sampl...
Accidental infection by Trypanosoma cruzi follow-up by the polymerase chain rea...
Kinoshita-Yanaga, Andréa Tieko; Toledo, Max Jean de Ornelas; Araújo, Silvana Marques de; Vier, Berenice Pelizza; Gomes, Mônica LúciaWe report a case of accidental infection by Trypanosoma cruzi in a 42-year-old female patient who presented an inoculation chagoma. Laboratory confirmation was based on examination of fresh blood, Giemsa-stained blood smear, immunoenzyme test (EIA-IgG), indirect immunofluorescence (IIF-IgM, IgG) and polymerase chain reaction (PCR). Only the PCR gave a positive result, and the EIA test was inconclusive. Two trea...