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Outlook in tissue-engineered magnetic systems and biomagnetic control
Gonçalves, A I; Gomes, Manuela E.The advancement of tissue engineering strategies has opened up new therapeutic avenues in the regeneration of many musculoskeletal tissues and cell niches. The burst of research in nanotechnology associated with tissue engineering brings inputs for the precise control of cells and cellular environments, that can play an important role in the development of these new therapies. Magnetic actuation, especially in ...
Controlling Macrophage Polarization to Modulate Inflammatory Cues Using Immune-...
Almeida, A. F.; Miranda, M. S.; Vinhas, Carla Adriana Araújo; Gonçalves, A I; Gomes, Manuela E.; Rodrigues, Márcia T.The persistence of inflammatory mediators in tissue niches significantly impacts regenerative outcomes and contributes to chronic diseases. Interleukin-4 (IL4) boosts pro-healing phenotypes in macrophages (MÏ ) and triggers the activation of signal transducer and activator of transcription 6 (STAT6). Since the IL4/STAT6 pathway reduces MÏ responsiveness to inflammation in a targeted and precise manner, IL4 del...
The impact of cryopreservation in signature markers and immunomodulatory profil...
Gonçalves, A I; Vinhas, Carla Adriana Araújo; Rodrigues, Márcia T.; Gomes, Manuela E.Tendon and ligament (T/L) engineering strategies towards clinical practice have been challenged by a paucity of understanding in the identification and still poorly described characterization of cellular niches. Prospecting how resident cell populations behave in vitro, and how cryopreservation may influence T/ L-promoting factors, can provide insights into T/ L-cellular profiles for novel regenerative solut...
Magnetic triggers in biomedical applications – prospects for contact free cell ...
Almeida, A. F.; Vinhas, Carla Adriana Araújo; Gonçalves, A I; Miranda, Margarida Silva; Rodrigues, Márcia T.; Gomes, Manuela E.In recent years, the inputs from magnetically assisted strategies have been contributing to the development of more sensitive screening methods and precise means of diagnosis to overcome existing and emerging treatment challenges. The features of magnetic materials enabling in vivo traceability, specific targeting and space- and time-controlled delivery of nanomedicines have highlighted the resourcefulness of...
Hyaluronic acid oligomer immobilization as an angiogenic trigger for the neovas...
Silva, A. L.; Babo, Pedro Miguel Sousa; Rodrigues, Márcia T.; Gonçalves, A I; Novoa-Carballal, Ramon; Pires, R. A.; Rouwkema, J.; Reis, R. L.Tissue engineered (TE) substitutes of clinically relevant sizes need an adequate vascular system to ensure function and proper tissue integration after implantation. However, the predictable vascularization of TE substitutes is yet to be achieved. Molecular weight variations in hyaluronic acid (HA) have been pointed to trigger angiogenesis. Thus, this study investigates HA oligomer immobilization as a promoter ...
Pulsed electromagnetic field modulates tendon cells response in il‐1β‐condition...
Vinhas, Adriana; Rodrigues, Márcia T.; Gonçalves, A I; Reis, R. L.; Gomes, Manuela E.Strategies aiming at controlling and modulating inflammatory cues may offer therapeutic solutions for improving tendon regeneration. This study aims to investigate the modulatory effect of pulsed electromagnetic field (PEMF) on the inflammatory profile of human tendonâ derived cells (hTDCs) after supplementation with interleukinâ 1β (ILâ 1β). ILâ 1β was used to artificially induce in-flammatory cues associ...
Magnetic responsive materials modulate the inflammatory profile of IL-1β condit...
Vinhas, A.; Rodrigues, Márcia T.; Gonçalves, A I; Reis, R. L.; Gomes, Manuela E.Tendinopathies represent half of all musculoskeletal injuries worldwide. Inflammatory events contribute to both tendon healing and to tendinopathy conditions but the cellular triggers leading to one or the other are unknown. In previous studies, we showed that magnetic field actuation modulates human tendon cells (hTDCs) behavior in pro-inflammatory environments, and that magnetic responsive membranes could pos...
Bioinspired materials and tissue engineering approaches applied to the regenera...
Rodrigues, Márcia T.; Gonçalves, A I; Babo, Pedro Miguel Sousa; Gomez-Florit, Manuel; Reis, R. L.; Gomes, Manuela E.The musculoskeletal tissues have a prime role in the biomechanical support and metabolic activities of the human body. As musculoskeletal tissues are highly prone to injuries, conditions afflicting these tissues have a great impact on the quality of life of patients worldwide. Tissue engineering approaches hold the promise to develop bioengineered substitutes aiming at the regeneration of failing and injured ti...
Magnetic stimulation drives macrophage polarization in cell to–cell communicati...
Vinhas, Adriana; Almeida, Ana Filipa Martins; Gonçalves, A I; Rodrigues, Márcia T.; Gomes, Manuela E.Inflammation is part of the natural healing response, but it has been simultaneously associated with tendon disorders, as persistent inflammatory events contribute to physiological changes that compromise tendon functions. The cellular interactions within a niche are extremely important for healing. While human tendon cells (hTDCs) are responsible for the maintenance of tendon matrix and turnover, macrophages r...
Evaluation of tenogenic differentiation potential of selected subpopulations of...
Gonçalves, A I; Berdecka, Dominika; Rodrigues, Márcia T.; Eren, Aysegul Dede; Boer, Jan de; Reis, R. L.; Gomes, Manuela E.Identification of a suitable cell source and bioactive agents guiding cell differentiationtowards tenogenic phenotype represents a prerequisite for advancement of cellâ based therapies for tendon repair. Human adiposeâ derived stem cells (hASCs) are apromising, yet intrinsically heterogenous population with diversified differentiationcapacities. In this work, we investigated antigenicallyâ defined subsets of hA...