40 documents found, page 1 of 4
Extraction and purification of biopolymers from marine origin sources envisagin...
Carvalho, Duarte Nuno; Gonçalves, Cristiana; Sousa, Rita Alexandra Oliveira; Reis, R. L.; Oliveira, Joaquim M.; Silva, Tiago H.Biopolymers are a versatile and diverse class of materials that has won high interest due to their potential application in several sectors of the economy, such as cosmetics, medical materials/devices, and food additives. In the last years, the search for these compounds has explored a wider range of marine organisms that have proven to be a great alternative to mammal sources for these applications and benefit...
Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced p...
Radhouani, Hajer; Gonçalves, Cristiana; Maia, F. Raquel; Oliveira, Maria Eduarda Moreira Pinheiro Santos; Reis, R. L.; Oliveira, Joaquim M.Hydrogels based on natural polysaccharides can have unique properties and be tailored for several applications, which may be mainly limited by the fragile structure and weak mechanical properties of this type of system. We successfully prepared cryogels made of newly synthesized kefiran exopolysaccharide-chondroitin sulfate (CS) conjugate via carbodiimide-mediated coupling to overcome these drawbacks. The freez...
Engineering of viscosupplement biomaterials for treatment of osteoarthritis: A ...
Gonçalves, Cristiana; Carvalho, Duarte Nuno; Silva, Tiago H.; Reis, R. L.; Oliveira, Joaquim M.Osteoarthritis (OA) is a progressive degenerative disease that causes severe pain and functional limitation, especially during locomotion. It is the most common arthritis type that damages the surface of articular cartilage until the underlying bone. In the past decade, the scientific community has made a considerable effort to improve or discover therapeutical products used as a form of conservative treatment ...
Impact of kefiran exopolysaccharide extraction on its applicability for tissue ...
Correia, Susana; Gonçalves, Cristiana; Oliveira, Joaquim M.; Radhouani, Hajer; Reis, R. L.Kefiran is an exopolysaccharide produced by the microflora of kefir grains used to produce the fermented milk beverage kefir. The health-promoting and physicochemical properties of kefiran led to its exploration for a range of applications, mainly in the food industry and biomedical fields. Aiming to explore its potential for tissue engineering and regenerative medicine (TERM) applications, the kefiran biopolym...
A design of experiments (DoE) approach to optimize cryogel manufacturing for ti...
Carvalho, Duarte Nuno; Gonçalves, Cristiana; Oliveira, J. M.; Williams, David S.; Mearns-Spragg, Andrew; Reis, R. L.; Silva, Tiago H.Marine origin polymers represent a sustainable and natural alternative to mammal counterparts regarding the biomedical application due to their similarities with proteins and polysaccharides present in extracellular matrix (ECM) in humans and can reduce the risks associated with zoonosis and overcoming social- and religious-related constraints. In particular, collagen-based biomaterials have been widely explore...
Fabrication of biocompatible porous SAIB/silk fibroin scaffolds using ionic liq...
Gonçalves, Cristiana; Gomes, J. M.; Maia, F. Raquel; Radhouani, Hajer; Silva, S. S.; Reis, R. L.; Oliveira, J. M.Sucrose acetate isobutyrate (SAIB) is a highly viscous hydrogel and presents many challenges for processing when its application in tissue engineering is envisioned. Herein, is proposed an innovative methodology by processing SAIB with ionic liquids (ILs), combined with silk fibroin (SF), to develop SAIB/SF scaffolds. Fourier Transform Infrared analysis confirmed SF and SAIB presence in the structures and the e...
PAMAM dendrimers functionalised with an anti-TNF α antibody and chondroitin sul...
Oliveira, Isabel Maria Lopes Matos; Gonçalves, Cristiana; Oliveira, Maria Eduarda Moreira Pinheiro Santos; Simón-Váz, Rosana; da Silva Morais, AlainRheumatoid arthritis is a chronic autoimmune disease characterised by joint synovial inflammation, along with cartilage and bone tissue destruction. Dendrimers can offer new opportunities as drug delivery systems of molecules of interest. Herein we aimed to develop poly(amidoamine) dendrimers (PAMAM), functionalised with chondroitin sulphate (CS), lined with anti-TNF α antibodies (Abs) to provide anti-inflammat...
Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels...
Radhouani, Hajer; Correia, Susana; Gonçalves, Cristiana; Reis, R. L.; Oliveira, J. M.Hydrogel application feasibility is still limited mainly due to their low mechanical strength and fragile nature. Therefore, several physical and chemical cross-linking modifications are being used to improve their properties. In this research, methacrylated Kefiran was synthesized by reacting Kefiran with methacrylic anhydride (MA). The developed MA-Kefiran was physicochemically characterized, and its biologic...
Promising biomolecules
Oliveira, Isabel M.; Carvalho, Ana L.; Radhouani, Hajer; Gonçalves, Cristiana; Oliveira, J. M.; Reis, R. L.The osteochondral defect (OD) comprises the articular cartilage and its subchondral bone. The treatment of these lesions remains as one of the most problematic clinical issues, since these defects include different tissues, requiring distinct healing approaches. Among the growing applications of regenerative medicine, clinical articular cartilage repair has been used for two decades, and it is an effective exam...
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the progr...
Ribeiro, Viviana P.; Silva-Correia, Joana; Gonçalves, Cristiana; Pina, Sandra; Radhouani, Hajer; Montonen, Toni; Oliveira, Joaquim M.; Oliveira, Ana L.Timely and spatially-regulated injectable hydrogels, able to suppress growing tumors in response to conformational transitions of proteins, are of great interest in cancer research and treatment. Herein, we report rapidly responsive silk fibroin (SF) hydrogels formed by a horseradish peroxidase (HRP) crosslinking reaction at physiological conditions, and demonstrate their use as an artificial biomimetic three-d...