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Bioprospecting bioactive peptides in Halobatrachus didactylus Body Mucus: From ...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Maria ManuelaFish body mucus plays a protective role, especially in Halobatrachus didactylus, which inhabits intertidal zones vulnerable to anthropogenic contaminants. In silico predicted bioactive peptides were identified in its body mucus, namely, EDNSELGQETPTLR (HdKTLR), DPPNPKNL (HdKNL), PAPPPPPP (HdPPP), VYPFPGPLPN (HdVLPN), and PFPGPLPN (HdLPN). These peptides were studied in vitro for bioactivities and aggregation be...
Bioprospecting bioactive peptides in halobatrachus didactylus body mucus: from ...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Maria ManuelaFish body mucus plays a protective role, especially in Halobatrachus didactylus, which inhabits intertidal zones vulnerable to anthropogenic contaminants. In silico predicted bioactive peptides were identified in its body mucus, namely, EDNSELGQETPTLR (HdKTLR), DPPNPKNL (HdKNL), PAPPPPPP (HdPPP), VYPFPGPLPN (HdVLPN), and PFPGPLPN (HdLPN). These peptides were studied in vitro for bioactivities and aggregation be...
High variability in aggression and habituation to the mirror assay in ornamenta...
Silva, Melina Coelho da; Canário, Adelino V. M.; Hubbard, Peter C.; Cardoso, Sara D.; Gonçalves, David; da Silveira Gonçalves, David MiguelUnderstanding consistent inter-individual variability in animal behaviour, known as personality traits, is essential for exploring the mechanisms and evolutionary consequences of behavioural diversity. Aggressive behaviour influences survival, resource acquisition, and reproduction, so clarifying individual differences can enhance our understanding of ecological dynamics and improve experimental design accuracy...
A Vida Secreta da Galinhola: Investigar Esta Esquiva Espécie Cinegética em São ...
O'Hehir, Sophie; Pavão, Diogo C.; Rodrigues, Tiago M.; Leitão, Manuel; Silva, Luís F.; Gonçalves, DavidA galinhola (Scolopax rusticola) é uma espécie cinegética importante nos Açores e em toda a Europa. Contudo, em São Miguel, infelizmente, é uma ave raramente avistada. A sua caça na ilha não é permitida há décadas, uma vez que o seu efetivo é muito reduzido. A população de galinhola nos Açores é residente, o que significa que não migra sazonalmente, para se reproduzir ou invernar. Com todo o seu ciclo de vida p...
Exploring the biodegradation of PET in mangrove soil and its intermediates by e...
Saidu, Muhammad Bashir; Moreira, Irina S.; Amorim, Catarina L.; Wu, Rongben; Ho, Yuen-Wa; Fang, James Kar-Hei; Castro, Paula M. L.; Gonçalves, DavidThe biodegradation of Polyethylene terephthalate (PET) is important due to the environmental impact of plastic waste. This study investigates the degradation of PET films in soil microcosms, with and without mangrove plants, and with mangrove plants bioaugmented with a bacterial consortium (Bacillus sp.- GPB12 and Enterococcus sp.- WTP31B-5) while following the evolution of soil microcosm microbiome. The abilit...
Comparative analysis of antioxidant and fatty acid composition in avocado (Pers...
Gonçalves, David; Gouveia, Carla S. S.; Ferreira, Maria J.; Ganança, José F. T.; Pinto, Diana C. G.; Carvalho, Miguel A. A. Pinheiro dehytochemical and biochemical properties. Aiming to evaluate the antioxidant quality and fatty acid composi tion with a commercial avocado, flours were obtained from five varieties (four regional and one commercial Hass) across different tissues (pulp and by-products) and cycles (years and on-tree maturation stages). Results showed that a regional variety with thin purple skin had the highest antioxidant qualiti...
Bioaccessibility of novel bioactive peptides from the body mucus of the Lusitan...
Fernandez Cunha, Marta; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe bioprospection of marine resources for drug discovery is receiving increasing attention (1). Adverse marine environmental conditions lead organisms to develop a collection of bioactive molecules for survival (1). Mucus, acting as a first line of defense against pathogens (2), is known to protect fish from the surrounding environment. Our previous studies have already demonstrated the bioactive potential of ...
NanoLC-MS/MS sequencing and in silico analysis of bioactive peptides from Lusit...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe mucus covering a fish's body works as a protective barrier and contains molecules, such as antimicrobial peptides, that defend against pathogens. In this study, two adult Halobatrachus didactylus were captured from the wild in Sesimbra, and mucus was collected by scraping the dorsal-lateral body with a sponge. Our objective was to identify new bioactive peptides in mucus samples via chromatography. SE-HPLC ...
Sustainable bioprospecting and circular economy approach: fractionation and ide...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaBioactive molecules from marine organisms, especially the Halobatrachus didactylus, also known as the Lusitanian toadfish, native to the Portuguese coast, remains largely unexplored. It is important to use sustainable bioprospecting practices to protect both the species and its ecosystem (1). Therefore, by adopting a circular economy approach, we can maximize the sustainable use of marine resources by sampling ...
Bioaccessibility of novel bioactive peptides from the body mucus of the Lusitan...
Fernandez Cunha, Marta; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe bioprospection of marine resources for drug discovery is receiving increasing attention (1). Adverse marine environmental conditions lead organisms to develop a collection of bioactive molecules for survival (1). Mucus, acting as a first line of defense against pathogens (2), is known to protect fish from the surrounding environment. Our previous studies have already demonstrated the bioactive potential of ...