18 documents found, page 1 of 2
AI, docking, and molecular dynamics to track the binding of structural peptides...
Ferreira, Tiago; Pereira, Francisco; Gonçalves, Filipa; Costa, André da; Cavaco-Paulo, Artur; Castro, Tarsila GabrielThe present work shows a computational approach to assess the interactions of different nature-inspired peptides with hair keratin models. An updated keratin model was validated, and comparisons with previous models were traced, thereby highlighting the time-evolution of both the model and the in silico methods. Importantly, the computational methodology presented here allows for the study of peptide-protein in...
Peptide-keratin interactions for enhanced hair properties
Carvalho, José; Rodrigues, Ana Rita O.; Ferreira, Tiago; Gomes, Vânia; Pereira, Francisco; Martins, Cátia D. F.; Martins, MadalenaSummary Fusion peptides inspired by natural elastomeric proteins are fused to a keratin peptide (KP) and tested on damaged hair. Here, we report thermal and mechanical tests conducted on treated damaged hair in conjunction with the peptides physicochemical properties, resulting in a statistical multiparametric analysis. Among the parameters, the Youngs modulus and the keratin helix denaturation temperature show...
Influence of the Preparation Method on the Structural, Morphological and Dielec...
Devesa, Susana; Gonçalves, Filipa; Graça, ManuelIn this work, iron niobate (FeNbO4) was prepared via two processes based on the sol-gel method: colloidal gel and polymeric gel. The obtained powders were submitted to heat treatments at different temperatures based on the results obtained via differential thermal analysis. The structures of the prepared samples were characterized via X-ray diffraction and the morphology was characterized via scanning electron ...
Guia orientador para a análise das PSF à luz da fonologia não linear
Fernandes, Ana Margarida; Marques, Carolina; Gonçalves, Filipa; Afonso, Maria Alexandra; Baptista, Ana Catarina; Rodrigues, SusanaAs crianças com Perturbações dos Sons da Fala (PSF) constituem um grupo heterogéneo, uma vez que diferem entre si quanto à idade, gravidade, etiologia, tipo de erros de fala, envolvimento de outros aspetos do sistema linguístico, desenvolvimento e resposta ao tratamento, o que acarreta dificuldades na classificação, que são acrescidas pela ausência de guidelines claras de diagnóstico para a identificação de cri...
Biotechnological applications of mammalian odorant-binding proteins
Gonçalves, Filipa; Ribeiro, Artur; Silva, C.; Cavaco-Paulo, ArturThe olfactory system of mammals allows the detection and discrimination of thousands of odors from the environment. In mammals, odorant-binding proteins (OBPs) are considered responsible to carry odorant molecules across the aqueous nasal mucus to the olfactory receptors (ORs). The three-dimensional structure of these proteins presents eight antiparallel ?-sheets and a short ?-helical segment close to the C ter...
PINK1 : a bridge between mitochondria and Parkinson's disease
Gonçalves, Filipa; Morais, Vanessa A.Mitochondria are known as highly dynamic organelles essential for energy production. Intriguingly, in the recent years, mitochondria have revealed the ability to maintain cell homeostasis and ultimately regulate cell fate. This regulation is achieved by evoking mitochondrial quality control pathways that are capable of sensing the overall status of the cellular environment. In a first instance, actions to maint...
Zein impart hydrophobic and antimicrobial properties to cotton textiles
Gonçalves, J. A.; Torres, Natália; Silva, Sónia Carina; Gonçalves, Filipa; Noro, Jennifer Martins; Cavaco-Paulo, Artur; Ribeiro, Artur; Silva, C.In this work, zein, a by-product of the agricultural industry of corn, was used to impart hydrophobicity and antimicrobial activity to textiles. The cotton textiles were functionalized with zein in two different forms, free or as particles. The optimized coating conditions which rendered the highest hydrophobic character to the textiles were found to be 50g/L zein in the free form prepared with 70% ethanol. To ...
Catalytic activation of esterases by PEGylation for polyester synthesis
Noro, Jennifer; Castro, T.; Gonçalves, Filipa; Ribeiro, Artur; Cavaco-Paulo, Artur; Silva, CarlaIn this work we explored PEGylation as an efficient strategy to improve esterases catalytic performance. For this, we PEGylated three esterases, namely lipase from Candida antarctica B (CALB), lipase from Thermomyces lanuginosus (TL) and cutinase from Fusarium solani pisi (CUT) and evaluated their catalytic performance by using the biosynthesis of poly(ethylene glutarate) as model reaction. After PEGylation wit...
Fusion proteins with chromogenic and keratin binding modules
Tinoco, Ana; Antunes, Egipto; Martins, Madalena; Gonçalves, Filipa; Gomes, Andreia C; Silva, Carla; Cavaco-Paulo, Artur; Ribeiro, ArturThe present research relates to a fusion protein comprising a chromogenic blue ultramarine protein (UM) bound to a keratin-based peptide (KP). The KP-UM fusion protein explores UM chromogenic nature together with KP affinity towards hair. For the first time a fusion protein with a chromogenic nature is explored as a hair coloring agent. The KP-UM protein colored overbleached hair, being the color dependent on t...
Reversible water driven chirality inversion in cellulose-based helices isolated...
Almeida, Ana; Querciagrossa, Lara; Silva, Pedro; Gonçalves, Filipa; Canejo, João; Almeida, Pedro L.; Godinho, Maria Helena; Zannoni, ClaudioAmong the movements observed in some cellulosic structures produced by plants are those that involve the dispersion and burial of seeds, as for example in Erodium from the Geraniaceae plant family. Here we report on a simple and efficient strategy to isolate and tune cellulose-based hygroscopic responsive materials from Erodium awns’ dead tissues. The stimuli-responsive material isolated forms left-handed (L) o...