4 documents found, page 1 of 1
Highly fluorescent 2-amino-6-cyanopurines as new bioimaging tools for studying ...
Gonçalves, João N. D.; Gonçalves, Jorge M.; Rodrigues, L. R.; Coutinho, Paulo J. G.; Sá, Paulo Correia de; Castanheira, Elisabete M. S.Fluorescent nucleobase analogues (FNAs) have been extensively used for scientific and biomedical purposes owing to their relevant photophysical properties. In this study, five newly synthesized 2-amino-6-cyanopurines were evaluated for their fluorescent probing and imaging capabilities using Saccharomyces cerevisiae as model organism. The new compounds, which were synthesized using highly efficient cost-effecti...
2,4,5-Triaminopyrimidines as blue fluorescent probes for cell viability monitor...
Gonçalves, Jorge M.; Gonçalves, João N. D.; Sousa, Luís F.; Rodrigues, L. R.; Correia-de-Sá, Paulo; Coutinho, Paulo J. G.; Castanheira, Elisabete M. S.Monitoring cell viability is critical in cell biology, pathology, and drug discovery. Most cell viability assays are cell-destructive, time-consuming, expensive, and/or hazardous. Herein, we present a series of newly synthesized 2,4,5-triaminopyrimidine derivatives able to discriminate between live and dead cells. To our knowledge, these compounds are the first fluorescent nucleobase analogues (FNAs) with cell ...
Highly fluorescent 2‐Aminopurine derivatives: synthesis, photo‐physical charact...
Gonçalves, Jorge M.; Gonçalves, João N. D.; Pêra, Ana S.; Senhorães, Nádia R.; Rodrigues, Ana Rita Oliveira; Oliveira, Rui Pedro Soares deNew fluorescent nucleobase analogues (FBAs) are emerg-ing as extraordinarily useful tools for DNA labelling tech-nologies. The highly fluorescent adenine analogue 2 aminopurine (2AP) is still the most used within the few hundreds of newly FBAs synthesized, but its excitation in the UV region demands for high energy sources endanger-ing living cells. New and highly fluorescent 2AP deriva-tives, 2-amino-6-cyanopu...
Anti-inflammatory effect of Pterospartum tridentatum leaf extract in acute and ...
Laranjeira, Inês Martins; Gonçalves, João N. D.; Gonçalves, Cátia; Silva, Marlene; Mouta, Nuno; Dias, Alberto C. P.; Pinto-Ribeiro, FilipaPterospartum tridendatum is an important source of active compounds with anti-inflammatory properties. The ability of P. tridentatum leaves methanolic extract in preventing/reversing inflammation was studied in adult rats using a model of experimental osteoarthritis (OA) and ear edema. Control animals (SHAM) were administered phosphate buffer solution (PBS), while OA animals received either P. tridentatum 100 m...