7 documents found, page 1 of 1
How temperature modulates the expression of pathogenesis-related molecules of t...
Félix, Carina; Meneses, Rodrigo; Gonçalves, Micael F. M.; Duarte, Ana S.; Jorrín-Novo, Jesus V.; van de Peer, Yves; Deforce, DieterLasiodiplodia hormozganensis, initially recognized as a fungal plant pathogen, is recognized now acknowledged as a potential threat to humans. However, our understanding of the pathogenesis mechanisms of Lasiodiplodia species remains limited, and the impact of temperature on its pathogenicity is unclear. This study aims to elucidate the effects of temperature on the biology of L. hormozganensis, focusing on the...
Unveiling biological activities of marine fungi: the effect of sea salt
Gonçalves, Micael F. M.; Paço, Ana; Escada, Luís F.; Albuquerque, Manuela S. F.; Pinto, Carlos A.; Saraiva, Jorge A.; Duarte, Ana SofiaThere is an urgent need for new substances to overcome current challenges in the health sciences. Marine fungi are known producers of numerous compounds, but the manipulation of growth conditions for optimal compound production can be laborious and time-consuming. In Portugal, despite its very long coastline, there are only a few studies on marine fungi. From a collection of Portuguese marine fungi, we screened...
Dual RNA sequencing of vitis vinifera during lasiodiplodia theobromae infection...
Gonçalves, Micael F. M.; Nunes, Rui B.; Tilleman, Laurentijn; Peer, Yves Van De; Deforce, Dieter; Nieuwerburgh, Filip Van; Esteves, Ana C.; Alves, ArturLasiodiplodia theobromae is one of the most aggressive agents of the grapevine trunk disease Botryosphaeria dieback. Through a dual RNA-sequencing approach, this study aimed to give a broader perspective on the infection strategy deployed by L. theobromae, while understanding grapevine response. Approximately 0.05% and 90% of the reads were mapped to the genomes of L. theobromae and Vitis vinifera, respectively...
Neptunomyces aureus gen. et sp. nov. (Didymosphaeriaceae, Pleosporales) isolate...
Gonçalves, Micael F. M.; Vicente, Tânia F. L.; Esteves, Ana C.; Alves, ArturA collection of fungi was isolated from macroalgae of the genera Gracilaria, Enteromorpha and Ulva in the estuary Ria de Aveiro in Portugal. These isolates were characterized through a multilocus phylogeny based on ITS region of the ribosomal DNA, beta-tubulin (tub2) and translation elongation factor 1 alpha (tef1-a) sequences, in conjunction with morphological and physiological data. These analyses showed that...
A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae revea...
Félix, Carina; Meneses, Rodrigo; Gonçalves, Micael F. M.; Tilleman, Laurentijn; Duarte, Ana S.; Jorrín-Novo, Jesus V.; Peer, Yves Van deLasiodiplodia theobromae (Botryosphaeriaceae, Ascomycota) is a plant pathogen and human opportunist whose pathogenicity is modulated by temperature. The molecular effects of temperature on L. theobromae are mostly unknown, so we used a multi-omics approach to understand how temperature affects the molecular mechanisms of pathogenicity. The genome of L. theobromae LA-SOL3 was sequenced (Illumina MiSeq) and annot...
Enchytraeid Reproduction Test(PLUS): hatching, growth and full life cycle test-...
Bicho, Rita C.; Santos, Fátima C. F.; Gonçalves, Micael F. M.; Soares, Amadeu M. V. M.; Amorim, Mónica J. B.Soil ecotoxicity standard tests for invertebrates are usually limited to the assessment of endpoints like survival and reproduction. Adverse effects may occur at other developmental stages, e.g. embryo development, hatching or growth. The species Enchytraeus crypticus is a model organism in the standard soil ecotoxicology test, where survival and reproduction are assessed. In the present study we optimized the ...
Development of an embryotoxicity test for Enchytraeus crypticus: the effect of Cd
Gonçalves, Micael F. M.; Bicho, Rita C.; Rêma, Alexandra; Soares, Amadeu M. V. M.; Faustino, Augusto M. R.; Amorim, Mónica J. B.The existing standard enchytraeid reproduction test (ERT) concerns the assessment of effects on survival and reproduction. In the present study we optimized and propose an embryotoxicity test using Enchytraeus crypticus. Cadmium (Cd) was used as a test substance. Endpoints evaluated were embryo development, number of embryonic structures, Calcium (Ca) channels quantification and hatching success with macroscopi...