6 documents found, page 1 of 1
PO 111 - Síndrome QT longo, silencioso e potencialmente fatal - case report
Santos, Olga; Rosinha, Daniela; Gonçalves, Nádia; Maia, Paulox
Exercise preconditioning prevents left ventricular dysfunction and remodeling i...
Schmidt, Cristine; Bovolini, José A.; Gonçalves, Nádia; Vasques-Nóvoa, Francisco; Andrade, Maria do Amparo; Santos, Mário; Leite-Moreira, AdelinoBackground: Despite pulmonary arterial hypertension (PAH) directly affects the right ventricle (RV), important structural, functional, and molecular changes also occur in left ventricle (LV). The objective of our study was to analyze the hypothetical cardioprotective effects of exercise preconditioning on LV in rats with monocrotaline (MCT)-induced PAH. Methods: Forty male Wistar rats were randomly separated in...
Improving kinetic or thermodynamic stability of an azoreductase by directed evo...
Brissos, Vânia; Gonçalves, Nádia; Melo, Eduardo; Martins, Lígia O.Protein stability arises from a combination of factors which are often difficult to rationalise. Therefore its improvement is better addressed through directed evolution than by rational design approaches. In this study, five rounds of mutagenesis/recombination followed by high-throughput screening (approximate to 10,000 clones) yielded the hit 1B6 showing a 300-fold higher half life at 50 degrees C than that e...
Evolving the Thermostability of PpAzoR, an Azoreductase from Pseudomonas putida...
Gonçalves, NádiaDissertação de mestrado em Bioquímica, apresentada ao Departamento Ciências da Vida da Faculdade de Ciências e Tecnologia da Universidade de Coimbra; Os corantes azo são o maior grupo de corantes sintéticos utilizados na indústria, e a sua libertação no ambiente representa uma das maiores fontes de poluição a nível mundial. A PpAzoR é uma azoreductase NADPH-dependente de FMN da Pseudomonas putida MET94, capaz d...
Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibro...
Soares, João Bruno; Nunes, Pedro Pimentel; Afonso, Luís; Rolanda, Carla; Lopes, Paula; Roncon-Albuquerque, R.; Gonçalves, Nádia; Carvalho, Inês BoalWe evaluated expression of TLR2, TLR4 and proinflammatory genes [NF-?B, TNF-a, cyclooxygenase-2 (COX-2)] in liver samples of patients in different stages of liver disease. Fifteen patients with unexplained transaminases elevation (reference group), 22 with viral chronic hepatitis (hepatitis group), 14 with virus-induced severe fibrosis/cirrhosis (cirrhosis group) and 10 with hepatocarcinoma (hepatocarcinoma gro...
A diminuição da rigidez miocárdica induzida pela fenilefrina é modulada pela PKC
Sousa, Ana Fontes de; Conceição, Luísa; Cardoso, Vera; Gonçalves, Nádia; Silva, Carmen Brás; Moreira, Adelino Leite[abstract]