13 documents found, page 1 of 2
Nucleolin Overexpression Predicts Patient Prognosis While Providing a Framework...
Fernandes, Ângela Valério; Fonseca, Nuno A.; Gonçalves, Nélio; Cruz, Ana F.; Pereira, Marta I.; Gregório, Ana C.; Moura, Vera; Ladeirinha, Ana F.Notwithstanding the advances in the treatment of lung cancer with immune checkpoint inhibitors, the high percentage of non-responders supports the development of novel anticancer treatments. Herein, the expression of the onco-target nucleolin in patient-derived pulmonary carcinomas was characterized, along with the assessment of its potential as a therapeutic target. The clinical prognostic value of nucleolin f...
GMP-grade nanoparticle targeted to nucleolin downregulates tumor molecular sign...
Fonseca, Nuno A.; Gregório, Ana C.; Mendes, Vera M.; Lopes, Rui; Abreu, Teresa; Gonçalves, Nélio; Manadas, Bruno; Lacerda, Manuela; Figueiredo, PauloPatients with breast or ovarian cancer have not benefited from improved efficacy with pegylated liposomal doxorubicin relative to free drug, likely due to the limited extent of the enhanced permeability and retention (EPR) effect, further compromising drug bioavailability in the tumor. Herein it is hypothesized that targeting nucleolin overexpressed in tumor endothelial cells (readily accessible from the vascul...
Cancer Stem Cells and Nucleolin as Drivers of Carcinogenesis
Carvalho, Laura Sofia; Gonçalves, Nélio; Fonseca, Nuno André; Moreira, João NunoCancer, one of the most mortal diseases worldwide, is characterized by the gain of specific features and cellular heterogeneity. Clonal evolution is an established theory to explain heterogeneity, but the discovery of cancer stem cells expanded the concept to include the hierarchical growth and plasticity of cancer cells. The activation of epithelial-to-mesenchymal transition and its molecular players are widel...
The Enhanced Efficacy of Intracellular Delivery of Doxorubicin/C6-Ceramide Comb...
Cruz, Ana F.; Caleiras, Mariana B.; Fonseca, Nuno A.; Gonçalves, Nélio; Mendes, Vera M.; Sampaio, Susana F.; Moura, Vera; Melo, Joana B.Targeting multiple cellular populations is of high therapeutic relevance for the tackling of solid tumors heterogeneity. Herein, the ability of pegylated and pH-sensitive liposomes, functionalized with the nucleolin-binding F3 peptide and containing doxorubicin (DXR)/C6-ceramide synergistic combination, to target, in vitro, ovarian cancer, including ovarian cancer stem cells (CSC), was assessed. The underlying ...
Gênese e desenvolvimento histórico-filosófico do conceito de Ideologia em Marx ...
Gonçalves, NélioEsta pesquisa tem por objetivo compreender e explicar, em linhas gerais, o processo de desenvolvimento, histórico-filosófico, do conceito de Ideologia em Karl Marx (1818 – 1888) e Friedrich Engels (1820 – 1895) mais precisamente, a partir da obra A Ideologia Alemã. Desejamos compreender o que o faz ser um conceito tão caro ao marxismo e para a filosofia moderna, forte influência sobre o pensamento contemporâneo...
Neuronal Adenosine A2A Receptors Are Critical Mediators of Neurodegeneration Tr...
Canas, Paula M.; Porciúncula, Lisiane O.; Simões, Ana Patrícia; Augusto, Elisabete de Oliveira; Silva, Henrique B.; Machado, Nuno J.; Gonçalves, NélioNeurodegeneration is a process transversal to neuropsychiatric diseases and the understanding of its mechanisms should allow devising strategies to prevent this irreversible step in brain diseases. Neurodegeneration caused by seizures is a critical step in the aggravation of temporal lobe epilepsy, but its mechanisms remain undetermined. Convulsions trigger an elevation of extracellular adenosine and upregulate...
Adenosine A2A Receptors in the Rat Prelimbic Medial Prefrontal Cortex Control D...
Leffa, Douglas T; Pandolfo, Pablo; Gonçalves, Nélio; Machado, Nuno J.; de Souza, Carolina M; Real, Joana I.; Silva, Antonio C.; Silva, Henrique B.Adenosine A2A receptors (A2ARs) were recently described to control synaptic plasticity and network activity in the prefrontal cortex (PFC). We now probed the role of these PFC A2AR by evaluating the behavioral performance (locomotor activity, anxiety-related behavior, cost-benefit decision making and working memory) of rats upon downregulation of A2AR selectively in the prelimbic medial PFC (PLmPFC) via viral s...
Early synaptic deficits in the APP/PS1 mouse model of Alzheimer's disease invol...
Silva, Sílvia Viana da; Haberl, Matthias Georg; Zhang, Pei; Bethge, Philipp; Lemos, Cristina; Gonçalves, Nélio; Gorlewicz, Adam; Malezieux, MerylSynaptic plasticity in the autoassociative network of recurrent connections among hippocampal CA3 pyramidal cells is thought to enable the storage of episodic memory. Impaired episodic memory is an early manifestation of cognitive deficits in Alzheimer's disease (AD). In the APP/PS1 mouse model of AD amyloidosis, we show that associative long-term synaptic potentiation (LTP) is abolished in CA3 pyramidal cells ...
Depression as a Glial-Based Synaptic Dysfunction
Rial, Daniel; Lemos, Cristina; Pinheiro, Helena; Duarte, Joana M.; Gonçalves, Francisco Q.; Real, Joana I.; Prediger, Rui D.; Gonçalves, NélioRecent studies combining pharmacological, behavioral, electrophysiological and molecular approaches indicate that depression results from maladaptive neuroplastic processes occurring in defined frontolimbic circuits responsible for emotional processing such as the prefrontal cortex, hippocampus, amygdala and ventral striatum. However, the exact mechanisms controlling synaptic plasticity that are disrupted to tr...
Calpain inhibition reduces ataxin-3 cleavage alleviating neuropathology and mot...
Simões, Ana Teresa; Gonçalves, Nélio; Nobre, Rui Jorge; Duarte, Carlos Bandeira; Almeida, Luís Pereira deMachado–Joseph Disease (MJD) is the most prevalent autosomal dominantly inherited cerebellar ataxia. It is caused by an expanded CAG repeat in the ATXN3 gene, which translates into a polyglutamine tract within the ataxin-3 protein. Present treatments are symptomatic and do not prevent disease progression. As calpain overactivation has been shown to contribute to mutant ataxin-3 proteolysis, translocation to the...