105 documents found, page 1 of 11
Phytochemicals and quaternary phosphonium ionic liquids: Connecting the dots to...
Chavarria, D; Anabela Borges; Benfeito, S; Sequeira, L; Ribeiro, M; Oliveira, C; Borges, F; Simões, M; Cagide, F; Simões MIntroduction: The infections by multidrug-resistant bacteria are a growing threat to human health, and the efficacy of the available antibiotics is gradually decreasing. As such, new antibiotic classes are urgently needed. Objectives: This study aims to evaluate the antimicrobial activity, safety and mechanism of action of phytochemical-based triphenylphosphonium (TPP') conjugates. Methods: A library of phytoch...
Genetic and epigenetic alterations of cdh1 regulatory regions in hereditary and...
Tedaldi, G; Molinari, C; São José, C; Barbosa-Matos, R; André, A; Danesi, R; Arcangeli, V; Ravegnani, M; Saragoni, L; Morgagni, P; Rebuzzi, F; Canale, ME-cadherin is a key player in gastric cancer (GC) and germline alterations of CDH1, its encoding gene, are responsible for Hereditary Diffuse Gastric Cancer (HDGC) syndrome. This study aimed at elucidating the role of genetic variants and DNA methylation of CDH1 promoter and enhancers in the regulation of gene expression. For this purpose, we analyzed genetic variants of the CDH1 gene through Next-Generation Se...
Histological and mutational profile of diffuse gastric cancer: current knowledg...
Garcia-Pelaez, J; Barbosa-Matos, R; Gullo, I; Carneiro, F; Oliveira, CGastric cancer (GC) pathogenesis is complex and heterogeneous, reflecting morphological, molecular and genetic diversity. Diffuse gastric cancer (DGC) and intestinal gastric cancer (IGC) are the major histological types. GC may be sporadic or hereditary; sporadic GC is related to environmental and genetic low-risk factors and hereditary GC is caused by inherited high-risk mutations, so far identified only for t...
Proteomics analysis of gastric cancer patients with diabetes mellitus
Osorio, H; Silva, C; Ferreira, M; Gullo, I; Máximo, V; Barros, R; Mendonça, F; Oliveira, C; Carneiro, FProteomics is a powerful approach to study the molecular mechanisms of cancer. In this study, we aim to characterize the proteomic profile of gastric cancer (GC) in patients with diabetes mellitus (DM) type 2. Forty GC tissue samples including 19 cases from diabetic patients and 21 cases from individuals without diabetes (control group) were selected for the proteomics analysis. Gastric tissues were processed f...
Immunophenotype of gastric tumors unveils a pleiotropic role of regulatory t ce...
Rocha, S; Basto, AP; Ijsselsteijn, ME; Teles, SP; Azevedo, MM; Gonçalves, G; Gullo, I; Almeida, GM; Maqueda, JJ; Oliveira, MI; Carneiro, F; Barata, JTGastric cancer (GC) patients display increased regulatory T cell (Tregs) numbers in peripheral blood and among tumor-infiltrating lymphocytes. Nevertheless, the role of Tregs in GC progression remains controversial. Here, we sought to explore the impact of Tregs in GCs with distinct histology, and whether Tregs can directly influence tumor cell behavior and GC develop-ment. We performed a comprehensive immunoph...
Engineering modular half-antibody conjugated nanoparticles for targeting CD44v6...
Lourenço, BN; Pereira, RF; Barrias, CC; Fischbach, C; Oliveira, C; Granja, PLGastric cancer (GC) remains a major cause of death worldwide mainly because of the late detection in advanced stage. Recently, we proposed CD44v6 as a relevant marker for early detection of GC, opening new avenues for GC-targeted theranostics. Here, we designed a modular nanoscale system that selectively targets CD44v6-expressing GC cells by the site-oriented conjugation of a new-engineered CD44v6 half-antibody...
A Fast Alternative to Soft Lithography for the Fabrication of Organ-on-a-Chip E...
Ferreira, D; Rothbauer, M; Conde, JP; Ertl, P; Oliveira, C; Granja, PLOrgan-on-a-chip technology promises to revolutionize how pre-clinical human trials are conducted. Engineering an in vitro environment that mimics the functionality and architecture of human physiology is essential toward building better platforms for drug development and personalized medicine. However, the complex nature of these devices requires specialized, time consuming, and expensive fabrication methodolog...
The cdh1 c.1901c>t variant: A founder variant in the portuguese population w...
Barbosa-Matos, R; Leal Silva, R; Garrido, L; Aguiar, AC; Garcia-Pelaez, J; André, A; Seixas, S; Sousa, SP; Ferro, L; Vilarinho, L; Gullo, I; Devezas, VHereditary diffuse gastric cancer (HDGC) caused by CDH1 variants predisposes to early-onset diffuse gastric (DGC) and lobular breast cancer (LBC). In Northern Portugal, the unusually high number of HDGC cases in unrelated families carrying the c.1901C>T variant (formerly known as p.A634V) suggested this as a CDH1-founder variant. We aimed to demonstrate that c.1901C>T is a bona fide truncating variant inducing ...
How Relevant is the Systemic Oxytocin Concentration for Human Sexual Behavior? ...
Cera, N; Vargas-Cáceres, S; Oliveira, C; Monteiro, J; Branco, D; Pignatelli, D; Rebelo, SIntroduction: Despite its role in social cognition and affiliative behavior, less is known about the role played by oxytocin in human sexual behavior. Aim: In the present systematic review, we aimed to find the levels of oxytocin related to human sexual arousal and orgasm. Methods: We conducted the study according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. We ...
Cd44v6 high membranous expression is a predictive marker of therapy response in...
Almeida, GM; Pereira, C; Park, JH; Lemos, C; Campelos, S; Gullo, I; Martins, D; Gonçalves, G; Leitão, D; Neto, JL; André, A; Borges, C; Almeida, DIn gastric cancer (GC), biomarkers that define prognosis and predict treatment response remain scarce. We hypothesized that the extent of CD44v6 membranous tumor expression could predict prognosis and therapy response in GC patients. Two GC surgical cohorts, from Portugal and South Korea (n = 964), were characterized for the extension of CD44v6 membranous immuno-expression, clinicopathological features, patient...