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Purification, biochemical characterization, and amino acid sequence of a novel ...

Carneiro, Rômulo Farias; Torres, Renato Cézar Farias; Chaves, Renata Pinheiro; Vasconcelos, Mayron Alves de; Sousa, Bruno Lopes de

A new lectin from Aplysia dactylomela eggs (ADEL) was isolated by affinity chromatography on HClactivated Sepharose™ media. Hemagglutination caused by ADEL was inhibited by several galactosides, mainly galacturonic acid (Ka = 6.05 × 106 M−1 ). The primary structure of ADEL consists of 217 residues, including 11 halfcystines involved in five intrachain and one interchain disulfide bond, resulting in a molecular ...

Date: 2022   |   Origin: Oasisbr
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