21 documents found, page 1 of 3
Biomarkers of hypoxic-ischemic encephalopathy: a systematic review
Caramelo, Inês; Coelho, Margarida; Rosado, Miguel; Cardoso, Carla M P; Dinis, Alexandra; Duarte, Carlos B.; Grãos, Mário; Manadas, BrunoBackground Current diagnostic criteria for hypoxic–ischemic encephalopathy in the early hours lack objective measurement tools. Therefore, this systematic review aims to identify putative molecules that can be used in diagnosis in daily clinical practice (PROSPERO ID: CRD42021272610). Data sources Searches were performed in PubMed, Web of Science, and Science Direct databases until November 2020. English origin...
Hypothermia Does Not Boost the Neuroprotection Promoted by Umbilical Cord Blood...
Serrenho, Inês; Cardoso, Carla M.; Grãos, Mário; Dinis, Alexandra; Manadas, Bruno; Baltazar, GraçaNeonatal hypoxic-ischemic encephalopathy (HIE) is one of the leading causes of death and long-term disability in the perinatal period. Currently, therapeutic hypothermia is the standard of care for this condition with modest efficacy and strict enrollment criteria. Therapy with umbilical cord blood cells (UCBC) has come forward as a strong candidate for the treatment of neonatal HIE, but no preclinical studies ...
Posttranslational modifications of proteins are key features in the identificat...
Salazar, Ivan L.; Lourenço, Ana S. T.; Manadas, Bruno; Baldeiras, Inês; Ferreira, Cláudia; Teixeira, Anabela Claro; Mendes, Vera M.; Novo, Ana MargaridaMultiple sclerosis is an inflammatory and degenerative disease of the central nervous system (CNS) characterized by demyelination and concomitant axonal loss. The lack of a single specific test, and the similarity to other inflammatory diseases of the central nervous system, makes it difficult to have a clear diagnosis of multiple sclerosis. Therefore, laboratory tests that allows a clear and definite diagnosis...
Modulation of signaling pathways by DJ-1: An updated overview
Neves, Margarida; Grãos, Mário; Anjo, Sandra I.; Manadas, BrunoEfforts have been made to understand the physiological and pathological role of DJ-1, a Parkinson's disease (PD)-associated protein, to provide new insights into PD pathophysiology. Such studies have revealed several neuroprotective roles of DJ-1, from which its ability to modulate signaling pathways seems to be of utmost importance for cell death regulation by DJ-1. Indeed, research on these topics has led to ...
Induced pluripotent stem cell-derived vascular networks to screen nano-bio inte...
Estronca, Luís; Francisco, Vitor; Pitrez, Patrícia; Honório, Inês; Carvalho, Lara; Vazão, Helena; Blersh, Josephine; Rai, Akhilesh; Nissan, XavierThis work was funded by FEDER through the Program COMPETE and by Portuguese fund through FCT in context of the projects PTDC/SAU-TOX/121887/2010, POCI-01-0145-FEDER-029229, POCI-01-0145-FEDER-016390 and POCI-01-0145-FEDER-029414, as well as the European project ERAatUC (ref. 669088). PRP wishes to thank FCT for a BD fellowship (SFRH/BD/71042/2010). The iMM Fish Facility was supported by CONGENTO LISBOA-01-0145-...
Stem Cell Therapy for Neonatal Hypoxic-Ischemic Encephalopathy: A Systematic Re...
Serrenho, Inês; Rosado, Miguel; Dinis, Alexandra; M Cardoso, Carla; Grãos, Mário; Manadas, Bruno; Baltazar, Graça Maria FernandesNeonatal hypoxic-ischemic encephalopathy (HIE) is an important cause of mortality and morbidity in the perinatal period. This condition results from a period of ischemia and hypoxia to the brain of neonates, leading to several disorders that profoundly affect the daily life of patients and their families. Currently, therapeutic hypothermia (TH) is the standard of care in developing countries; however, TH is not...
Induced pluripotent stem cell-derived vascular networks to screen nano-bio inte...
Estronca, Luís; Francisco, Vitor; Pitrez, Patrícia; Honório, Inês; Carvalho, Lara; Vazão, Helena; Blersch, Josephine; Rai, Akhilesh; Nissan, XavierThe vascular bioactivity/safety of nanomaterials is typically evaluated by animal testing, which is of low throughput and does not account for biological differences between animals and humans such as ageing, metabolism and disease profiles. The development of personalized human in vitro platforms to evaluate the interaction of nanomaterials with the vascular system would be important for both therapeutic and r...
Cofilin-1 Is a Mechanosensitive Regulator of Transcription
Domingues, Catarina; Geraldo, A. Margarida; Anjo, Sandra I.; Matos, André; Almeida, Cláudio; Caramelo, Inês; Lopes-da-Silva, José A; Paiva, ArturThe mechanical properties of the extracellular environment are interrogated by cells and integrated through mechanotransduction. Many cellular processes depend on actomyosin-dependent contractility, which is influenced by the microenvironment's stiffness. Here, we explored the influence of substrate stiffness on the proteome of proliferating undifferentiated human umbilical cord-matrix mesenchymal stem/stromal ...
Distinct oligodendrocyte populations have spatial preference and different resp...
Floriddia, Elisa M.; Lourenço, Tânia; Zhang, Shupei; van Bruggen, David; Hilscher, Markus M.; Kukanja, Petra; Gonçalves Dos Santos, João P.Mature oligodendrocytes (MOLs) show transcriptional heterogeneity, the functional consequences of which are unclear. MOL heterogeneity might correlate with the local environment or their interactions with different neuron types. Here, we show that distinct MOL populations have spatial preference in the mammalian central nervous system (CNS). We found that MOL type 2 (MOL2) is enriched in the spinal cord when co...
A different vision of translational research in biomarker discovery: a pilot st...
Anjo, Sandra I.; Dos Santos, Patrícia Valério; Rosado, Luiza; Baltazar, Graça; Baldeiras, Inês; Pires, Diana; Gomes, Andreia; Januário, CristinaBackground: The identification of circulating biomarkers that closely correlate with Parkinson’s Disease (PD) has failed several times in the past. Nevertheless, in this pilot study, a translational approach was conducted, allowing the evaluation of the plasma levels of two mitochondrial-related proteins, whose combination leads to a robust model with potential diagnostic value to discriminate the PD patients f...