24 documents found, page 1 of 3
The role of vitamin C in the gene expression of oxidative stress markers in fib...
Bonucci,Jessica; Gragnani,Alfredo; Trincado,Marcelo Moraes; Vincentin,Victor; Correa,Silvana Aparecida Alves; Ferreira,Lydia MasakoAbstract Purpose: To assess the action of vitamin C on the expression of 84 oxidative stress related-genes in cultured skin fibroblasts from burn patients. Methods: Skin samples were obtained from ten burn patients. Human primary fibroblasts were isolated and cultured to be distributed into 2 groups: TF (n = 10, fibroblasts treated with vitamin C) and UF (n = 10, untreated fibroblasts). Gene expression analysis...
Aldefluor protocol to sort keratinocytes stem cells from skin
Noronha,Samuel Marcos Ribeiro; Gragnani,Alfredo; Pereira,Thiago Antônio Calado; Correa,Silvana Aparecida Alves; Bonucci,Jessica; Ferreira,Lydia MasakoAbstract Purpose: To investigate the use Aldefluor® and N, N - Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. Methods: Activated Aldefluor® aliquots were prepared and maintained at temperature between 2 to 8°C, or stored at -20°C. Next, the cells were collected following the standard protocol of sample preparation. Results:...
Keratinocyte growth factor and the expression of wound-healing-related genes in...
Chomiski,Verônica; Gragnani,Alfredo; Bonucci,Jéssica; Correa,Silvana Aparecida Alves; Noronha,Samuel Marcos Ribeiro de; Ferreira,Lydia MasakoABSTRACT PURPOSE: To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients. METHODS: Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls),...
TGF-β1 on induced osteogenic differentiation of human dermal fibroblast
Aloise,Antonio Carlos; Pereira,Max Domingues; Duailibi,Silvio Eduardo; Gragnani,Alfredo; Ferreira,Lydia MasakoPURPOSE: To evaluate the role of transforming growth factor beta 1 (TGF-β1) on the induced osteogenic differentiation of human dermal fibroblasts. METHODS: We performed four groups with cultured dermal fibroblasts according to the culture medium: CONTROL (DMEM culture medium); TGF-β1 (DMEM culture medium with 10 ng/ml of TGF-β1); OSTEOG (DMEM culture medium with 0.5 µg/ml of ascorbic acid, 10 mmol/l of β-glycer...
Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necr...
Noronha,Samuel Marcos Ribeiro de; Noronha,Silvana Aparecida Alves Corrêa de; Klepp,Anthony Gueratto; Ipolito,Michele Zampieri; Ferreira,Lydia MasakoPURPOSE: To evaluate the level of cytokines and keratinocyte growth factor (KGF) or Fibroblast Growth Factor 7 (FGF-7) in the culture medium of cultured human dermal fibroblasts from patients with large burn in comparison to small burn. METHODS: Fibroblasts of 10 patients (four large burns, four small burns and two controls) were initiated by the enzymatic method using collagenase. Cytokines and KGF in the supe...
Toll like receptors gene expression of human keratinocytes cultured of severe b...
Cornick,Sarita Mac; Noronha,Silvana Aparecida Alves Corrêa de; Noronha,Samuel Marcos Ribeiro de; Cezillo,Marcus V. B.; Ferreira,Lydia MasakoPURPOSE: To evaluate the expression profile of genes related to Toll Like Receptors (TLR) pathways of human Primary Epidermal keratinocytes of patients with severe burns. METHODS: After obtaining viable fragments of skin with and without burning, culture hKEP was initiated by the enzymatic method using Dispase (Sigma-Aldrich). These cells were treated with Trizol(r) (Life Technologies) for extraction of total R...
Human beta defensin-4 and keratinocyte growth factor gene expression in culture...
Noronha,Silvana Aparecida Alves Corrêa de; Noronha,Samuel Marcos Ribeiro de; Lanziani,Larissa Elias; Ipolito,Michele Zampieri; Ferreira,Lydia MasakoPURPOSE: To evaluate KGF and human beta defensin-4 (HBD-4) levels produced by dermic fibroblasts and keratinocytes cultivated from burned patients' skin samples. METHODS: Keratinocytes and fibroblasts of 10 patients (four major burns, four minor burns and two controls) were primarily cultivated according to standard methods. HBD-4 and KGF genes were analyzed by quantitative PCR. RESULTS: In fibroblasts, KGF gen...
Innate and adaptive immunity gene expression of human keratinocytes cultured of...
Noronha,Silvana Aparecida Alves Corrêa de; Noronha,Samuel Marcos Ribeiro de; Lanziani,Larissa Elias; Ferreira,Lydia Masako; Gragnani,AlfredoPURPOSE: Evaluate the expression profile of genes related to Innate and Adaptive Immune System (IAIS) of human Primary Epidermal keratinocytes (hPEKP) of patients with severe burns. METHODS: After obtaining viable fragments of skin with and without burning, culture hKEP was initiated by the enzymatic method using Dispase (Sigma-Aldrich). These cells were treated with Trizol(r) (Life Technologies) for extraction...
Oxandrolone use in adult burn patients. Systematic review and meta-analysis
Real,Daniel Sundfeld Spiga; Reis,Rodrigo Pacheco; Piccolo,Monica Sarto; Okamoto,Regina H.; Gragnani,Alfredo; Ferreira,Lydia M.PURPOSE: This study is a systematic literature review and meta-analysis concerning the use of a testosterone synthetic analog, oxandrolone, and its use in severe adult burns. METHODS: Randomized prospective clinical studies, in English, Portuguese or Spanish, were sought on the following databases: MEDLINE, COCHRANE, EMBASE and LILACS. There was no restriction in relation to the publication date. RESULTS: This ...
Unripe Musa sapientum peel in the healing of surgical wounds in rats
Atzingen,Dênia Amélia Novato Castelli Von; Gragnani,Alfredo; Veiga,Daniela Francescato; Abla,Luis Eduardo Felipe; Cardoso,Lorraine Lorene FelixPURPOSE: To assess the effects of unripe Musa sapientum peel on the healing of surgical wounds in rats. METHODS: One hundred and twenty Wistar rats were divided into two treatment groups of 60 animals each: the control group (gel without the active ingredient) and experimental group (4% Musa sapientum peel gel). A 4 x 4 cm surgical wound was created on the back of each animal. The wound was cleaned daily with 0...