2 documents found, page 1 of 1
Multiparametric in vitro genotoxicity assessment of different variants of amorp...
Brandão, Fátima; Costa, Carla; Bessa, Maria João; Valdiglesias, Vanessa; Hellack, Bryan; Haase, Andrea; Fraga, Sónia; Teixeira, João PauloThe hazard posed to human health by inhaled amorphous silica nanomaterials (aSiO2 NM) remains uncertain. Herein, we assessed the cyto- and genotoxicity of aSiO2 NM variants covering different sizes (7, 15, and 40 nm) and surface modifications (unmodified, phosphonate-, amino- and trimethylsilyl-modified) on rat alveolar epithelial (RLE-6TN) cells. Cytotoxicity was evaluated at 24 h after exposure to the aSiO2 N...
Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation ...
Brandão, Fátima; Costa, Carla; Bessa, Maria João; Dumortier, Elise; Debacq-Chainiaux, Florence; Hubaux, Roland; Salmon, Michel; Laloy, JulieSeveral reports on amorphous silica nanomaterial (aSiO2 NM) toxicity have been questioning their safety. Herein, we investigated the in vivo pulmonary toxicity of four variants of aSiO2 NM: SiO2_15_Unmod, SiO2_15_Amino, SiO2_7 and SiO2_40. We focused on alterations in lung DNA and protein integrity, and gene expression following single intratracheal instillation in rats. Additionally, a short-term inhalation st...