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Lower seroprevalence for SARS-CoV-2-specific antibodies among kidney transplant...

Cristelli, Marina P.; Viana, Laila A.; Fortaleza, Carlos M. [UNESP]; Granato, Celso; Nakamura, Monica R.; Santos, Daniel W.C.L.

Made available in DSpace on 2022-04-28T19:43:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01; Background: Kidney transplant recipients have higher COVID-19 associated mortality compared to the general population. However, as only symptomatic patients seek medical attention, the current level of exposure, the main sources of acquisition, and the behavior of humoral immunity over time are poorly u...

Date: 2022   |   Origin: Oasisbr


Comparison of the direct fluorescence assay and real-time polymerase chain reac...

Perosa, Ana Helena; Watanabe, Aripuana S.A.; Guatura, Sandra B.; Silva, Ellen R.M.; Granato, Celso; Bellei, Nancy

OBJECTIVE: This study evaluated the diagnostic performance of two methods for the detection of influenza virus in immunocompromised transplant patients. METHODS: A total of 475 respiratory samples, 236 from patients in a hematopoietic stem cell transplantation program and 239 from kidney transplant patients, were analyzed by a direct fluorescence assay and the Centers for Disease Control real-time polymerase ch...

Date: 2013   |   Origin: Oasisbr

Frequency of human bocavirus respiratory infections among at-risk patients in S...

Caccia, Elaine Regina Baptista; Watanabe, Aripuana Sakurada Aranha; Carraro, Emerson; Leal, Elcio; Granato, Celso; Bellei, Nancy

BACKGROUND AND OBJECTIVES: Human Bocavirus (HBoV) has been described since 2005 as an etiological agent of respiratory virus infections. From 2001 to 2008 we investigated the etiology of HBoV among adults and children in different groups at risk of presenting complications arising from acute respiratory infection, the investigation was carried out in a tertiary hospital health care system in Brazil. METHODS: HB...

Date: 2012   |   Origin: Oasisbr

Desenvolvimento de ELISA indireto simples e de baixo custo para detecção de ant...

Ono, Erika; Lafer, Manuel Mindlin; Weckx, Lily Yin; Granato, Celso; Moraes-Pinto, Maria Isabel de

We have developed a cheaper an simple in house indirect ELISA that uses the live attenuated VZV vaccine as a coating antigen. The alternative ELISA had an agreement of 94% when compared with a commercial VZV ELISA kit. Moreover, our ELISA proved to be more reliable than the kit when assessing true negative samples. By adding a standard serum, we were able to produce results in international units per millilitre...

Date: 2004   |   Origin: Oasisbr

Active immunization against hepatitis B virus (HBV) with low-doses of plasma-de...

Carrilho, Flair José; Queiroz, Maria Lúcia; Silva, Luiz Caetano da; Fonseca, Luís Edmundo Pinto da; Granato, Celso; Oba, Isabel; Obara, Leda

Schedule for vaccination against HBV infection has usually been based on three separate injections of 20 meg of the vaccine by intramuscular route. One of the main shortcomings to its use in large scale programs has been its high cost. Ninety out of 300 health workers were submitted to three injections of 2 meg of plasma-derived vaccine (PDV) by intradermal (ID) route on days 0, 30, and 180. Anti-HBs was detect...

Date: 1989   |   Origin: Oasisbr

An ELISA method using serum derived HDAg for the sorological detection of HDV a...

Granato, Celso; Strauss, Edna; Vitvitsky, Ludmila; Gayotto, Luiz Carlos da Costa; Trepo, Christian

One of the main difficulties related to the detection of the Hepatitis Delta Virus (HDV) antigen and antibody has been the source of the needed HD antigen since HDV containing human and animal livers are very difficult to obtain and since yield is low. This fact prompted us to try to use the serum of patients in the acute phase of HDV infection as a source of HDAg and turn to enzyme immunoassays (EIA) instead o...

Date: 1987   |   Origin: Oasisbr

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