6 documents found, page 1 of 1
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlorora...
Pereira, João R.; Araújo, Diana; Freitas, Patrícia; Marques, Ana C.; Delgado Alves, Vitor; Sevrin, Chantal; Grandfils, Christian; Fortunato, ElviraPseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp, a glucose- and fructoserich waste generated during juice production, to produce medium-chain length polyhydroxyalkanoates. A cell dry mass of 8.74 ± 0.20 g/L, with a polymer content of 49.25 ± 4.08% were attained. The produced biopolymer was composed of 42.7±0.1mol% 3-hydroxydecanoate, 17.9±1.0mol% 3-hydroxyoctanoate, 14.5±1.1mol...
Demonstration of the ability of the bacterial polysaccharide FucoPol to floccul...
Araújo, Diana; Concórdio-Reis, Patrícia; Marques, Ana C.; Sevrin, Chantal; Grandfils, Christian; Delgado Alves, VitorIn this study, the flocculation properties of FucoPol, a bacterial extracellular polysaccharide, were investigated. FucoPol is a high molecular weight polymer and negatively charged due to the presence of glucuronic acid and the acyl groups succinyl and pyruvyl. High flocculation rate values (>70%) were achieved with a low bioflocculant dosage of 1 mg/L, for pH values in the range 3–5 and temperature within 15–...
Preparation and characterization of films based on a natural P(3HB)/mcl-PHA ble...
Rebocho, Ana Teresa; Pereira, João R.; Neves, Luísa A.; Delgado Alves, Vitor; Sevrin, Chantal; Grandfils, Christian; Freitas, Filomena; Reis, Maria A.M.The co-culture of Cupriavidus necator DSM 428 and Pseudomonas citronellolis NRRL B-2504 was performed using apple pulp waste from the fruit processing industry as the sole carbon source to produce poly(3-hydroxybutyrate), P(3HB) and medium-chain length PHA, mcl-PHA, respectively. The polymers accumulated by both strains were extracted from the co-culture’s biomass, resulting in a natural blend that was composed...
Preparation and Characterization of Films Based on a Natural P(3HB)/mcl-PHA Ble...
Rebocho, Ana Teresa; Pereira, João R.; Neves, Luísa A.; Delgado Alves, Vitor; Sevrin, Chantal; Grandfils, Christian; Freitas, Filomena; Reis, Maria A.M.The co-culture of Cupriavidus necator DSM 428 and Pseudomonas citronellolis NRRL B-2504 was performed using apple pulp waste from the fruit processing industry as the sole carbon source to produce poly(3-hydroxybutyrate), P(3HB) and medium-chain length PHA, mcl-PHA, respectively. The polymers accumulated by both strains were extracted from the co-culture’s biomass, resulting in a natural blend that was composed...
Chitin-glucan complex production by Komagataella pastoris: Downstream optimizat...
Farinha, Ines; Duarte, Paulo; Pimentel, Ana; Plotnikova, Evgeniya; Chagas, Barbara; Mafra, Luis; Grandfils, Christian; Freitas, FilomenaPurified chitin-glucan complex (CGC(pure)) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionized water. The copolymer thus obtained had a beta-glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGC(pure) had an average molecular weight of 4.9 x 10(5) Da with a p...
Solution properties of an exopolysaccharide from a Pseudomonas strain obtained ...
Hilliou, L.; Freitas, Filomena; Oliveira, Rui; Reis, Maria A.M.; Lespineux, David; Grandfils, Christian; Alves, Vítor D.We report the solution properties of a new exopolysaccharide (EPS) obtained from a Pseudomonas strain fed with glycerol as the sole source of carbon. This high molecular mass (3 x 10(6) g mol(-1)) biopolymer is essentially made of galactose monomers with pyruvate and succinate groups imparting a polyelectrolyte character. The Smidsrod parameter B computed from the ionic strength dependence of the intrinsic visc...