15 documents found, page 1 of 2
Secretory production in Escherichia coli of a GH46 chitosanase from Chromobacte...
Azevedo, Mayara I. G.; Oliveira, Simone T.; Silva, Christiana F. B.; Carneiro, Rômulo F.; Nagano, Celso S.; Gadelha, Ana Clarice da SilvaA chitosanase (CvCsn46) from Chromobacterium violaceum ATCC 12472 was produced in Escherichia coli, purified, and partially characterized. When subjected to denaturing polyacrylamide gel electrophoresis, the enzyme migrated as two protein bands (38 and 36 kDa apparent molecular masses), which were both identified as CvCsn46 by mass spectrometry. The enzyme hydrolyzed colloidal chitosan, with optimum catalytic a...
Structural and enzymatic characterization of Peruvianin‑I, the first germin-lik...
Cruz, Wallace T. da; Bezerra, Eduardo H. S.; Grangeiro, Thalles B.; Lopes, Jose L. S.; Silva, Maria Z. R.; Ramos, Márcio Viana; Rocha, Bruno A. M.The germin-like protein (GLP) purified from Thevetia peruviana, Peruvianin-I, is the only one described as having proteolytic activity. Therefore, the goal of this study was to investigate the structural features responsible for its enzymatic activity. Although the amino acid sequence of Peruvianin-I showed high identity with other GLPs, it exhibited punctual mutations, which were responsible for the absence of...
Mo-CBP3, an antifungal Chitin-Binding Protein from Moringa oleifera Seeds, is a...
Freire, José E. C.; Vasconcelos, Ilka M.; Moreno, Frederico B. M. B.; Batista, Adelina B.; Lobo, Marina D. P.; Pereira, Mirella L.; Lima, João P. M. S.Mo-CBP3 is a chitin-binding protein from M. oleifera seeds that inhibits the germination and mycelial growth of phytopathogenic fungi. This protein is highly thermostable and resistant to pH changes, and therefore may be useful in the development of new antifungal drugs. However, the relationship of MoCBP3 with the known families of carbohydrate-binding domains has not been established. In the present study, fu...
New insights into the structure and mode of action of Mo-CBP3, an antifungal Ch...
Batista, Adelina B.; Oliveira, José T. A.; Gifoni, Juliana M.; Pereira, Mirella L.; Almeida, Marina G. G.; Gomes, Valdirene M.; Cunha, Maura daMo-CBP3 is a chitin-binding protein purified from Moringa oleifera Lam. seeds that displays inhibitory activity against phytopathogenic fungi. This study investigated the structural properties and the antifungal mode of action of this protein. To this end, circular dichroism spectroscopy, antifungal assays, measurements of the production of reactive oxygen species and microscopic analyses were utilized. Mo-CBP3...
Identification, characterization, and antifungal activity of cysteine peptidase...
Freitas, Cleverson D. T.; Silva, Rafaela O; Ramos, Márcio Viana; Porfírio, Camila T. M. N.; Farias, Davi F.; Sousa, Jeanlex S; Oliveira, João P. B.Cysteine peptidases (EC 3.4.22) are the most abundant enzymes in latex fluids. However, their physiological functions are still poorly understood, mainly related to defense against phytopathogens. The present study reports the cDNA cloning and sequencing of five undescribed cysteine peptidases from Calotropis procera (Aiton) Dryand (Apocynaceae) as well as some in silico analyses. Of these, three cysteine pepti...
Biotechnological potential of a cysteine protease (CpCP3) from Calotropis proce...
Silva, Maria Z. R.; Oliveira, João P. B.; Ramos, Márcio Viana; Farias, Davi F.; Sá, Chayenne A. de; Ribeiro, Juliana A. C.; Silva, Ayrles F. B.This article reports the characterization and evaluation of the biotechnological potential of a cysteine protease purified from Calotropis procera (CpCP3). This enzyme was highly stable to different metal ions and was able to hydrolyze κ-casein similarly to bovine chymosin. Atomic force microscopy showed that the process of casein micelle aggregation induced by CpCP3 was similar to that caused by chymosin. The ...
Structural and functional features of a class VI chitinase from cashew (Anacard...
Oliveira, Simone T.; Azevedo, Mayara I. G.; Cunha, Rodrigo M. S.; Silva, Christiana F. B.; Muniz, Celli R.; Monteiro-Júnior, José E.A partial cDNA sequence from Anacardium occidentale CCP 76 was obtained, encoding a GH19 chitinase (AoChi) belonging to class VI. AoChi exhibits distinct structural features in relation to previously characterized plant GH19 chitinases from classes I, II, IV and VII. For example, a conserved Glu residue at the catalytic center of typical GH19 chitinases, which acts as the proton donor during catalysis, is repla...
cDNA cloning, molecular modeling and docking calculations of L-type lectins fro...
Maranhão, Paulo A.C.; Teixeira, Claudener S.; Sousa, Bruno L.; Barroso-Neto, Ito L.; Monteiro-Júnior, José E.; Fernandes, Andreia V.; Ramos, Marcio V.The genus Swartzia is a member of the tribe Swartzieae, whose genera constitute the living descendants of one of the early branches of the papilionoid legumes. Legume lectins comprise one of the main families of structurally and evolutionarily related carbohydrate-binding proteins of plant origin. However, these proteins have been poorly investigated in Swartzia and to date, only the lectin from S. laevicarpa s...
Purification of a Lectin from the Marine Red Alga Gracilaria cornea and Its Eff...
Lima, Maria E. P.; Carneiro, Maria E.; Nascimento, Antônia E.; Grangeiro, Thalles B.; Holanda, Marjory L.; Amorim, Rodrigo C. N.; Benevides, Norma M. B.A lectin was purified from the seaweed Gracilaria cornea by hydrophobic interaction chromatography on phenyl-Sepharose CL-4B followed by affinity chromatography on immobilized mucin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of G. cornea lectin (GCL) revealed a single protein band of approximately 60 kDa, whereas by gel filtration on Sephadex G-100 its native molecular mass was 66 kDa. GCL exhib...
Relationships in subtribe Diocleinae (Leguminosae; Papilionoideae) inferred fro...
Varela, Eduardo S.; Lima, João P. M. S.; Galdino, Alexsandro S.; Pinto, Luciano da S.; Bezerra, Walderly M.; Nunes, Edson P.; Alves, Maria A. O.The complete sequences of nuclear ribosomal DNA (nrDNA) internal transcribed spacer regions (ITS/5.8S) were determined for species belonging to six genera from the subtribe Diocleinae as well as for the anomalous genera Calopogonium and Pachyrhizus. Phylogenetic trees constructed by distance matrix, maximum parsimony and maximum likelihood methods showed that Calopo- gonium and Pachyrhizus were outside the clad...