Encontrados 21 documentos, a visualizar página 1 de 3

Expression in Escherichia coli of cysteine protease inhibitors from cowpea (Vig...

Two cysteine proteinase inhibitors from cowpea, VuCys1 and VuCys2, were produced in E. coli ArcticExpress (DE3). The recombinant products strongly inhibited papain and chymopapain as well as the midgut proteases from Callosobruchus maculatus larvae, a bruchid that uses cysteine proteases as major digestive enzymes. Heat treatment at 100 °C for up to 60 min or incubation at various pH values caused little reduct...

Crystal structure of an antifungal osmotin-like protein from Calotropis procera...

CpOsm is an antifungal osmotin/thaumatin-like protein purified from the latex of Calotropis procera. The protein is relatively thermostable and retains its antifungal activity over a wide pH range; therefore, it may be useful in the development of new antifungal drugs or transgenic crops with enhanced resistance to phytopathogenic fungi. To gain further insight into the mechanism of action of CpOsm, its three-d...

Swine and poultry pathogens: The complete genome sequences of two strains of My...

This work reports the results of analyses of three complete mycoplasma genomes, a pathogenic (7448) and a nonpathogenic (J) strain of the swine pathogen Mycoplasma hyopneumoniae and a strain of the avian pathogen Mycoplasma synoviae; the genome sizes of the three strains were 920,079 bp, 897,405 bp, and 799,476 bp, respectively. These genomes were compared with other sequenced mycoplasma genomes reported in the...

The complete genome sequence of Chromobacterium violaceum reveals remarkable an...

Chromobacterium violaceum is one of millions of species of free-living microorganisms that populate the soil and water in the extant areas of tropical biodiversity around the world. Its complete genome sequence reveals (i) extensive alternative pathways for energy generation, (ii) ≈500 ORFs for transport-related proteins, (iii) complex and extensive systems for stress adaptation and motility, and (iv) wide-spre...

Clonagem, sequenciamento e expressão do gen da lectina (Con Br) de sementes de ...

The effect of several glucose/mannose specific lectins from Diocleinae legumes on the survivorship of Nilaparvata lugens was investigated. The gene coding for the most insecticidal lectin was cloned, sequenced and expressed in Escherichia coli cells. In addition, the molecular and three-dimensional structure of the lectin with the best insecticidal properties were investigated by N-terminal sequencing, mass spe...

In vitro evaluation of the curcumin against fluconazole-resistant strains of Ca...

The isolates of Candida spp. and Cryptococcus neoformans were inhibited by curcumin, with different degrees of inhibition among species, with MICs ranging from 8-64 μg/mL. After exposure of the C. albicans strains to curcumin, it was observed a decrease in the number of viable cells and damage to cell membranes, the mitochondrial function of C. albicans cells appears to be affected after exposure to curcumin. O...

Chitinases as antibacterial proteins: a systematic review

Chitinases are hydrolases that catalyze the cleavage of the β -1,4-O- glycosidic linkages in chitin, a polysaccharide abundantly found in nature. Chitin is an important structural component of the cell wall of most fungi and the exoskeleton of arthropods, including insects and crustaceans. These enzymes are widespread in the living world, being found in organisms from all three domains of life. Due to their hyd...

A chitin-binding protein purified from Moringa oleifera seeds presents anticand...

Candida species are opportunistic pathogens that infect immunocompromised and/or immunosuppressed patients, particularly in hospital facilities, that besides representing a significant threat to health increase the risk of mortality. Apart from echinocandins and triazoles, which are well tolerated, most of the antifungal drugs used for candidiasis treatment can cause side effects and lead to the development of ...

Expression in Escherichia coli of cysteine protease inhibitors from cowpea (Vig...

Antimicrobial effects of violacein against planktonic cells and biofilms of Sta...

Violacein is an indole compound, produced by Chromobacterium violaceum, a bacteria present in tropical and subtropical areas. Among its numerous biological activities, its antimicrobial potential stands out. This study aims to determine the antimicrobial activity of VIO on S. aureus in planktonic culture and biofilms. VIO showed excellent antimicrobial activity in inhibiting and killing S. aureus in planktonic ...