82 documents found, page 1 of 9
Impact of a daily legume-based meal on blood and anthropometric parameters in a...
Ferreira, Helena; Vasconcelos, Marta; Gil, Ana M.; Silveira, Joana; Alves, Paulo; Martins, Sandra; Assunção, Marco; Guimarães, João TiagoThis pilot study aimed to assess the impact of substituting a traditional lunch for a vegetarian legume-based meal on blood and anthropometric parameters in a group of omnivorous adults. A one-group comparison, quasi-experimental dietary intervention was designed. A vegetarian legume-based meal was offered for 8 consecutive weeks (weekdays) to non-vegetarian individuals (n = 26), (28 years [P25 = 20.0, P75 = 35...
Urine metabolomics during a legume diet intervention suggests altered metabolic...
Ferreira, Helena; Duarte, Daniela; Rodrigues, João A.; Vasconcelos, Marta W.; Pinto, Elisabete; Gil, Ana M.Given the general increase in legume consumption worldwide, there is a need to characterize the resulting human metabolic adaptations in order to demonstrate potential legume diet/health relationships. A nuclear magnetic resonance (NMR) metabolomics urine study was carried out on a small cohort (n = 18) to characterize the excretory effects of a pilot longitudinal 8-week legume-based dietary intervention. Despi...
Impact of a legumes diet on the human gut microbiome articulated with fecal and...
Ferreira, Helena; Duarte, Daniela; Carneiro, Tatiana J.; Costa, Célia; Barbosa, Joana C.; Rodrigues, João E.; Alves, Paulo; Vasconcelos, MartaBackground & aims: Legumes intake is known to be associated with several health benefits the origins of which is still a matter of debate. This paper addresses a pilot small cohort to probe for metabolic aspects of the interplay between legumes intake, human metabolism and gut microbiota. Methods: Untargeted nuclear magnetic resonance (NMR) metabolomics of blood plasma and fecal extracts was carried out, in tan...
Impact of Conventional and Potential New Metal-Based Drugs on Lipid Metabolism ...
Bispo, Daniela S. C.; Correia, Marlene; Carneiro, Tatiana J.; Martins, Ana S.; Reis, Aliana A. N.; Carvalho, Ana L. M. Batista de; Marques, Maria P. M.This work investigated the mechanisms of action of conventional drugs, cisplatin and oxaliplatin, and the potentially less deleterious drug Pd2Spermine (Spm) and its Pt(II) analog, against osteosarcoma MG-63 cells, using nuclear-magnetic-resonance metabolomics of the cellular lipidome. The Pt(II) chelates induced different responses, namely regarding polyunsaturated-fatty-acids (increased upon cisplatin), sugge...
Impact of Sea Warming and 17-α-Ethinylestradiol Exposure on the Lipid Metabolis...
Rodrigues, João A.; Bispo, Daniela S. C.; Silva, Mónica G.; Araújo, Rita; Soares, Amadeu M. V. M.; Freitas, Rosa; Gil, Ana M.This paper reports on an NMR metabolomics study of lipophilic extracts of Ruditapes philippinarum clams exposed to the hormonal contaminant 17-α-ethinylestradiol (EE2), at 17 °C and 21 °C. The results reveal that exposure at 17 °C triggers a weak response at low EE2 concentrations, suggestive of a slight increase in membrane rigidity, followed by lipid metabolic stability at higher EE2 concentrations. On the ot...
Metabolic Impact of Anticancer Drugs Pd2Spermine and Cisplatin on the Brain of ...
Carneiro, Tatiana J.; Vojtek, Martin; Gonçalves-Monteiro, Salomé; Neves, João R.; Carvalho, Ana L. M. Batista de; Marques, Maria Paula M.; Diniz, CarmenThe new palladium agent Pd2Spermine (Spm) has been reported to exhibit promising cytotoxic properties, while potentially circumventing the known disadvantages associated to cisplatin therapeutics, namely acquired resistance and high toxicity. This work presents a nuclear magnetic resonance (NMR) metabolomics study of brain extracts obtained from healthy mice, to assess the metabolic impacts of the new Pd2Spm co...
Nutritional, rheological, sensory characteristics and environmental impact of a...
Vieira, Evla D. F.; Styles, David; Sousa, Sérgio; Santos, Carla; Gil, Ana M.; Gomes, Ana M.; Vasconcelos, Marta W.Studies have demonstrated that the addition of pulses to foods can make them more nutritious. We hypothesize that lupin flour adds nutritional benefits to yogurts. This study aimed to characterize a lupin-enriched yogurt in nutritional, rheological, and sensorial terms by a trained panel, and assess its environmental impact using the life cycle assessment (LCA) approach. For comparison, natural yogurt and a com...
NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-der...
Bispo, Daniela S. C.; Jesus, Catarina S. H.; Correia, Marlene; Ferreira, Filipa; Bonifazio, Giulia; Goodfellow, Brian J.; Oliveira, Mariana B.This Article presents, for the first time to our knowledge, an untargeted nuclear magnetic resonance (NMR) metabolomic characterization of the polar intracellular metabolic adaptations of human adipose-derived mesenchymal stem cells during osteogenic differentiation. The use of mesenchymal stem cells (MSCs) for bone regeneration is a promising alternative to conventional bone grafts, and untargeted metabolomics...
Endo- and exometabolome crosstalk in mesenchymal stem cells undergoing osteogen...
Bispo, Daniela S. C.; Michálková, Lenka; Correia, Marlene; Jesus, Catarina S. H.; Duarte, Iola F.; Goodfellow, Brian J.; Oliveira, Mariana B.This paper describes, for the first time to our knowledge, a lipidome and exometabolome characterization of osteogenic differentiation for human adipose tissue stem cells (hAMSCs) using nuclear magnetic resonance (NMR) spectroscopy. The holistic nature of NMR enabled the time-course evolution of cholesterol, mono- and polyunsaturated fatty acids (including ω-6 and ω-3 fatty acids), several phospholipids (phosph...
An intracellular metabolic signature as a potential donor-independent marker of...
Bispo, Daniela S. C.; Jesus, Catarina S. H.; Romek, Katarzyna; Marques, Inês M. C.; Oliveira, Mariana B.; Mano, João F.; Gil, Ana M.This paper describes an untargeted NMR metabolomics study to identify potential intracellular donor-dependent and donor-independent metabolic markers of proliferation and osteogenic differentiation of human adipose mesenchymal stem cells (hAMSCs). The hAMSCs of two donors with distinct proliferating/osteogenic characteristics were fully characterized regarding their polar endometabolome during proliferation and...