13 documents found, page 1 of 2
Digestive and metabolic consequences of on-growing greater amberjack (Seriola d...
Navarro-Guillén, C.; Perera, E.; Pérez-Hilario, D.; Martos-Sitcha, J.A.; Molina-Roque, L.; Gregorio, Silvia; Fonseca, Filomena; Fuentes, J.; Yúfera, M.Aiming to elucidate the effects of temperature on different aspects of the fish digestive physiology that may affect the growth, we examined, firstly, variations in growth performance, feed intake, digestive enzyme activities, expression of transport related genes, metabolites in plasma and liver, and oxidative stress response in juveniles of greater amberjack (Seriola dumerili) growing during two months at 18,...
High plant protein diet impairs growth performance and intestinal integrity in ...
Fuentes, Juan; Fonseca, Filomena; Gregorio, Silvia; Kussaba Miguel, Larissa; Perera, Erick; Alarcón-López, Francisco J.; Martos-Sitcha, Juan A.The Mediterranean aquaculture industry that produces mainly seabream and seabass is exploring alternative plant-based protein sources for fish feeds together with the diversification of fish species. Effective plant-based formulations require diverse sources and additives to maintain fish welfare and growth, which is especially important for carnivorous species. In this scenario, the objective of the present st...
Effect of bile salts on intestinal epithelial function in gilthead seabream (Sp...
Fuentes, J.; Gregorio, Silvia; F. Fonseca; Robles-Arozarena, R.; Martos-Sitcha, J. A.; Moyano, F. J.In the context of modern aquaculture, the effort to reduce the reliance on fishmeal/marine ingredients in fish diets has led to the exploration of plant-based protein sources as potential substitutes, a dietary shift that disrupts the bile acid profile in fish. Therefore, bile salts are being sought as additives. However, artificially increased intestinal levels of bile acids may significantly impact mucosal fu...
Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream...
Ruiz-Jarabo, I.; Gregorio, Silvia; Fuentes, XoanCalcium balance is of paramount importance for vertebrates. In fish, the endocrine modulators of calcium homeostasis include the stanniocalcin (STC), and some members of the parathyroid hormone (PTH) family, such as the PTH-related protein (PTHrP), acting as antagonists. STC is ubiquitously expressed in higher vertebrates. In turn, bony fish exhibit specific STC-producing glands named the corpuscles of Stannius...
Dysregulation of intestinal physiology by Aflatoxicosis in the Gilthead Seabrea...
Barany, Andre; Oliva, Milagrosa; Gregorio, Silvia; Martínez-Rodríguez, Gonzalo; Mancera, Juan Miguel; Fuentes, JuanAflatoxin B1 (AFB1) is a mycotoxin often present in food. This study aimed to understand the physiological effects of AFB1 on the seabream (Sparus aurata) gastrointestinal system. In a first in vitro approach, we investigated ion transport using the short-circuit current (Isc) technique in Ussing chambers in the anterior intestine (AI). Application of apical/ luminal AFB1 concentrations of 8 and 16μM to healthy...
Increased intestinal carbonate precipitate abundance in the sea bream (Sparus a...
Gregorio, Silvia; Ruiz-Jarabo, Ignacio; Carvalho, Edison S. M.; Fuentes, JuanMarine fish contribute to the carbon cycle by producing mineralized intestinal precipitates generated as by-products of their osmoregulation. Here we aimed at characterizing the control of epithelial bicarbonate secretion and intestinal precipitate presence in the gilthead sea bream in response to predicted near future increases of environmental CO2. Our results demonstrate that hypercapnia (950 and 1800 μatm C...
Regulation of bicarbonate secretion in marine fish intestine by the calcium-sen...
Gregorio, Silvia; Fuentes, JuanIn marine fish, high epithelial intestinal HCO3- secretion generates luminal carbonate precipitates of divalent cations that play a key role in water and ion homeostasis. The present study was designed to expose the putative role for calcium and the calcium-sensing receptor (CaSR) in the regulation of HCO3- secretion in the intestine of the sea bream (Sparus aurata L.). Effects on the expression of the CaSR in ...
AVT is involved in the regulation of ion transport in the intestine of the sea ...
Martos-Sitcha, J. A.; Gregorio, Silvia; Carvalho, Edison Samir Mascarelhas; Canario, Adelino V. M.; Power, Deborah; Mancera, J. M.The intestine of marine fish plays a crucial role in ion homeostasis by selective processing of ingested fluid. Although arginine vasotocin (AVT) is suggested to play a role in ion regulation in fish, its action in the intestine has not been demonstrated. Thus, the present study investigated in vitro the putative role of AVT in intestinal ion transport in the sea bream (Sparus aurata). A cDNA encoding part of a...
Adaptation to different salinities exposes functional specialization in the int...
Gregorio, Silvia; Carvalho, Edison Samir Mascarelhas; Encarnação, Sandra; Wilson, J.; Power, Deborah; Canario, Adelino V. M.; Fuentes, J.The processing of intestinal fluid, in addition to a high drinking rate, is essential for osmoregulation in marine fish. This study analyzed the long-term response of the sea bream (Sparus aurata L.) to relevant changes of external salinity (12, 35 and 55p.p.t.), focusing on the anterior intestine and in the less-often studied rectum. Intestinal water absorption, epithelial HCO3– secretion and gene expression ...
Prolactin regulates luminal bicarbonate secretion in the intestine of the sea b...
Ferlazzo, A.; Carvalho, Edison Samir Mascarelhas; Gregorio, Silvia; Power, Deborah; Canario, Adelino V. M.; Tischitta, F.; Fuentes, J.The pituitary hormone prolactin is a pleiotropic endocrine factor that plays a major role in the regulation of ion balance in fish, with demonstrated actions mainly in the gills and kidney. The role of prolactin in intestinal ion transport remains little studied. In marine fish, which have high drinking rates, epithelial bicarbonate secretion in the intestine produces luminal carbonate aggregates believed to pl...