8 documents found, page 1 of 1
3D tubular constructs based on natural polysaccharides and recombinant polypept...
Rodrigues, Luísa Cidália Guimarães; Gomes, J. M.; Soares da Costa, Diana; Fernandes, Emanuel Mouta; Costa, R. R.; Rodríguez-Cabello, J. C.; Silva, S. S.The development of versatile tubular structures is critical for tissue engineering (TE) applications where vascularization is necessary. This study investigates the fabrication of tubular shaped biomaterials focused on chitosan (CHT) combined with alginate (ALG) and acemannan (ACE), known for their synergistic properties, including physical stability, antibacterial activity, and healing promotion. Translating t...
Microfluidic-assisted interfacial complexation of extracellular matrix componen...
Costa, R. R.; Caballero, David; Soares da Costa, Diana; Rodriguez-Trujillo, R.; Kundu, Subhas C; Reis, R. L.; Pashkuleva, I.Anisotropy is an important cue for neural organization during morphogenesis and healing, contributing to the mechanical and functional properties of neural tissues. The ability to replicate such anisotropy in vitro holds great promise for the development of effective regeneration strategies. In this work, interfacial polyelectrolyte complexation (IPC) is applied to fabricate microfibers from charged ECM compone...
Hyaluronan functionalized pH-responsive calcium carbonate nanoparticles for loc...
Bastos, F. R.; Costa, R. R.; Soares da Costa, Diana; Reis, R. L.; Pashkuleva, I.; Alves, N. M.[Excerpt] Introduction: Current radio- and chemotherapies are not efficient and many tumors remain resistant to conventional cancer treatments. Specific properties and signaling molecules from tumor microenvironment (TME) have been explored to increase treatment efficacy. An example is the acidic pH of the TME that has been explored to develop stimuli responsive release systems. Herein, we obtained biocompatibl...
Liposomes embedded in layer by layer constructs as simplistic extracellular ves...
Domínguez-Arca, V.; Costa, R. R.; Carvalho, Ana Margarida Moura Pereira Ferreira; Taboada, P.; Reis, R. L.; Prieto, G.; Pashkuleva, I.Extracellular vesicles (EVs) are particles originating from the exfoliation of the cellular membrane. They are involved in cell-to-cell and cell-to-matrix signaling, exchange of bioactive molecules, tumorigenesis and metastasis, among others. To mitigate the limited understanding of EVs transfer phenomena, we developed a simplistic model that mimics EVs and their interactions with cells and the extracellular ma...
Tuning the stiffness of surfaces by assembling genetically engineered polypepti...
Costa, R. R.; González-Pérez, M.; Herrero-Gutiérrez, M.; Pires, R. A.; Alonso, M.; Rodríguez-Cabello, J. C.; Reis, R. L.; Pashkuleva, I.We introduce elastin-like recombinamers (ELRs) as polypeptides with precise amino acid positioning to generate polypeptide coatings with tunable rigidity. Two ELRs are used: V84-ELR, a hydrophobic monoblock, and EI-ELR, an amphiphilic diblock. Both were modified with the amine-reactive tetrakis (hydroxymethyl) phosphonium chloride compound. We evaluated the affinity, the conformation, and the dissipative behavi...
Modos de ver e de dar a ver os Painéis de São Vicente
André, P.; Henriques, L. L.; Martinho, L. I.; Apolinário, S.; Costa, R. R.O artigo Modos de ver e de dar a ver os Painéis de São Vicente centra-se na ideia de que o conhecimento histórico articulado com as novas tecnologias pode ser uma forma de apropriação e de afirmação da pintura e da cultura portuguesa. A análise e a perceção dos contextos que determinaram os sucessivos percursos espaciais dos Painéis dentro e fora do Museu Nacional de Arte Antiga (Lisboa) e das sucessivas variaç...
Development of biomimetic and smart coatings based on elastin-like polymers con...
Costa, R. R.; Testera, A. M.; Arias, F. J.; Mano, J. F.; Rodríguez-Cabello, J. C.[Excerpt] Elastin-like polymers (ELPs) are based on repeating sequences found in the natural elastin. They show remarkable combination of properties, such as a large range of mechanical properties, excellent biocompatibility, and smart behaviour and self-assembly towards temperature, pH and often different stimuli. Around a certain temperature, known as inverse transition temperature (ITT) these polymers show p...
Development of biomimetic and smart coatings based on elastin-like polymers con...
Costa, R. R.; Testera, A. M.; Arias, F. J.; Mano, J. F.; Rodríguez-Cabello, J. C.Elastin-like polymers (ELPs) are based on repeating sequences found in the natural elastin. They show remarkable combination of properties, such as a large range of mechanical properties, excellent biocompatibility, and smart behaviour and self-assembly towards temperature, pH and often different stimuli. Around a certain temperature, known as inverse transition temperature (ITT) these polymers show phase trans...