2 documents found, page 1 of 1
Microtubule end conversion mediated by motors and diffusing proteins with no in...
Chakraborty, M; Tarasovetc, EV; Zaytsev, AV; Godzi, M; Figueiredo, AC; Ataullakhanov, FI; Grishchuk, ELAccurate chromosome segregation relies on microtubule end conversion, the ill-understood ability of kinetochores to transit from lateral microtubule attachment to durable association with dynamic microtubule plus-ends. The molecular requirements for this conversion and the underlying biophysical mechanisms are elusive. We reconstituted end conversion in vitro using two kinetochore components: the plus end–direc...
Microtubule detyrosination guides chromosomes during mitosis
Barisic, M; Sousa, RS; Tripathy, SK; Magiera, MM; Zaytsev, AV; Pereira, AL; Janke, C; Grishchuk, EL; Maiato, HBefore chromosomes segregate into daughter cells, they align at the mitotic spindle equator, a process known as chromosome congression. Centromere-associated protein E (CENP-E)/Kinesin-7 is a microtubule plus-end-directed kinetochore motor required for congression of pole-proximal chromosomes. Because the plus-ends of many astral microtubules in the spindle point to the cell cortex, it remains unknown how CENP-...