Genomic-based surveillance on the occurrence of drug resistance and its transmission dynamics has emerged as a powerful tool for the control of tuberculosis (TB). A whole-genome sequencing approach, phenotypic testing and clinical-epidemiological investigation were used to undertake a retrospective population-based study on drug-resistant (DR)-TB in Rio Grande do Sul, the largest state in Southern Brazil. The a...
Background and Objectives: Fluoroquinolones represent a class of amtimicrobials which isfrequently prescribed for the treatment of urinary tract infections (UTIs) of both hospital and community origin. This study aims, to determine the frequency and the factors associated with resistance to ciprofloxacin and levofloxacin in gram-negative bacilli isolated from patients with UTIs treated in a hospital in southern...
Background and objectives: Despite its use as a genotyping method for different microorganisms, few studies have reported the use of Multiple-Locus Variable Number of Tandem Repeats Analysis (MLVA) for the analysis of the clonal diversity of Helicobacter pylori. The aim of this study was to determine the genetic variability of H. pylori strains by MLVA in southern Brazil. Methods: 95 H. pylori DNA samples were ...
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A transfecção gênica tem sido eficientemente realizada a partir de diferentes formulações de lipídios catiônicos e neutros. Este trabalho teve como objetivo verificar a viabilidade de uma teoria que propõem utilizar moléculas anfifílicas como neutralizadoras da carga do DNA para a transfecção gênica in vitro. Foram realizadas transfecções utilizando o surfactante brometo de dodeciltrimetil amônio (DOTAB) ou o l...