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Serological screening for Chagas disease in an endemic region of Northern Minas...
Cruz , Dardiane Santos; Souza, Núbia Nunes de; Rafael, Aline Ferreira; Damasceno, Renata Fiuza; Ribeiro, Antonio Luiz Pinho; Oliveira, Léa Campos deChagas disease (CD) is still a neglected disease. Infected individuals are diagnosed late, being treated in worse clinical conditions. Thus, this study aimed to analyze the prevalence and the factors associated with new confirmed cases of CD identified by serological screening in an endemic region of Minas Gerais State, Brazil. This is an analytical cross-sectional study with data from a project of the Research...
Lack of evidence of seronegative infection in an endemic area of Chagas disease
Oliveira, Léa Campos de; Lee, Tzong-Hae; Ferreira, Ariela Mota; Bierrenbach, Ana Luiza; Souza-Basqueira, Marcela de; Oliveira, Cláudia Di LorenzoThe diagnosis of Chagas disease is based on the detection of Trypanosoma cruzi (T. cruzi)-specific antibodies. Nonetheless, there is concern about the sensitivity of current serological assays due to reports of T. cruzi PCR positivity among seronegative individuals. The aim of this study was to evaluate if T. cruzi seronegative infections occur in endemic areas. We recruited 2,157 individuals that were identifi...
An alternative storage method for characterization of the intestinal microbiota...
Ribeiro, Roberto Marques; Souza-Basqueira, Marcela de; Oliveira, Léa Campos de; Salles, Flavia Cristina; Pereira, Natalia Bueno; Sabino, Ester CerdeiraGut microbiota has been the subject of various molecular studies mainly due to its importance and wide-ranging relationships with human hosts. However, the storage of fecal samples prior to DNA extraction is critical when characterizing the composition of intestinal microbiota. Therefore, we aimed to understand the effects of different fecal storage methods to characterize intestinal microbiota using Next Gener...
Interleukin 10 (IL10) and transforming growth factor Beta 1 (TGF beta1) gene po...
Oliveira, Léa Campos de
Detecção de DNA de Bartonella henselae em amostras clínicas, incluindo sangue p...
Kawasato, Karina Hatamoto; Oliveira, Léa Campos de; Velho, Paulo Eduardo Neves Ferreira; Yamamoto, Lidia; Del Negro, Gilda Maria BarbaroBacteria of the genus Bartonella are emerging pathogens detected in lymph node biopsies and aspirates probably caused by increased concentration of bacteria. Twenty-three samples of 18 patients with clinical, laboratory and/or epidemiological data suggesting bartonellosis were subjected to three nested amplifications targeting a fragment of the 60-kDa heat shock protein (HSP), the internal transcribed spacer 16...